Sulistiyani

Single Nucleotide Polymorphism within the Promoter Region of Cynomolgus Monkey LDLR Gene

The cynomolgus monkey (Macaca fascicularis) is one of the most common nonhuman primate s used as an animal model in biomedical research relat ed to atherosclerosis. However, little is known about genetic variation in the low density lipoprotein receptor (LDLR) gene , its effect on cholesterol levels , and associated risk s of atherosclerosis. The refore this study aimed to identify genetic polymorphisms , namely single nucleotide polymorphism s (SNP s ) , within the promoter region of LDLR and their relation ship with animal responsiveness to hypercholesterolemia due to an atherogenic diet. In this research, SNPs were studied using DNA isolated from 22 cynomolgus monkeys obtained from a previous study, consisting of two hyporesponders, 19 hyperresponders, and one extreme case . The result showed that two SNP s existed in the promoter region , namely g.−169T>C and g.−265G>A. SNP g.−265G>A showed linkages with extreme responsive ness and can be used as a potential genetic marker for extreme animals. The result of this study has extended our knowledge of polymorphism in the LDLR gene and its use in the selection of cynomolgus monkeys as animal models in research on hypercholesterolemia and atherosclerosis.

Variasi Kolesterol Plasma Individual Monyet Ekor Panjang (Macaca fascicularis) sebagai Respons terhadap Diet Aterogenik IPB-1 [INDIVIDUAL PLASMA CHOLESTEROL VARIATION OF CYNOMOLGUS MACAQUE (MACACA FASCICULARIS) IN RESPONSE TO IPB-1 ATHEROGENIC DIET]

The effectiveness of atherogenic diet in raising plasma cholesterol level of cynomolgus macaques(Macaca fascicularis) can be predicted for population, but not for individuals.This study aimed to evaluatethe individual plasma cholesterol of cynomolgus macaques in response to IPB-1 atherogenic diet. Theanimals under study were 22 adult malemonkeys from the animal facility of Primate Research CenterBogor Agricultural University (PSSP IPB). All animals were intervened with the IPB-1 atherogenic diet forthree months and individual plasma cholesterol wasevaluated in a monthly basis.The results showedthat the monkeys’ plasma cholesterol had increased significantly (P <0.05) after one month of interventionperiod and the increases were very significantly different (P <0.01) after three months. Individually, increasesin plasma cholesterol varied among animals. Based on these variations, 2 animals were categorised ashypo-response, 18 animals as hyper-response and 2 animals as extreme.This study showed the variationof individual plasma cholesterol of cynomolgus macaques in response tothe IPB-1 atherogenic diet so thatthe animals might be selected based on the their responsiveness.