Sun-Yup Shim

Ecklonia cava extract suppresses the high-affinity IgE receptor, FCεRI expression

Basophils and mast cells express FcepsilonRI, a high-affinity receptor for IgE, on the cell surface and act as effector cells in allergic reactions. In this study, we investigated the inhibitory effect of Ecklonia cava (EC) methanolic extract on the expression of FcepsilonRI in human basophilic KU812F cells. Flow cytometric analysis revealed that EC extract caused a concentration-dependent reduction in the cell surface expression of FcepsilonRI. The extract was also capable of reducing the binding between IgE or serum IgE and cell surface FcepsilonRI. RT-PCR analysis revealed that EC extract reduced the mRNA expression of total cellular FcepsilonRI alpha-chain. Moreover, data obtained by fluorescence spectrophotometry showed that the extract inhibited the FcepsilonRI-mediated release of histamine in a concentration-dependent manner. These results suggest that EC extract may exert its anti-allergic activity through negative-regulation of FcepsilonRI expression and a decrease in histamine release.

Inhibitory effects of phloroglucinol derivatives isolated from Ecklonia stolonifera on FcεRI expression

Two bioactive phloroglucinol derivatives, dioxinodehydroeckol (DHE) and phlorofucofuroeckol A (PFF-A) were isolated from edible marine brown alga, Ecklonia stolonifera, and evaluated for effects on cell surface Fc(epsilon)RI expression in KU812F cells. DHE and PFF-A were found to reduce the cell surface expression, and total cellular protein and mRNA levels for the Fc(epsilon)RI alpha chain. Moreover, both compounds exerted inhibitory effects against the elevation of intracellular calcium concentration [Ca(2+)](i) and histamine release from anti-Fc(epsilon)RI alpha chain antibody (CRA-1)-stimulated cells. These inhibitory effects were stronger for PFF-A than for DHE. These results show that two phloroglucinol derivatives, DHE and PFF-A, may exert anti-allergic effects via the inhibition of Fc(epsilon)RI expression, calcium influx, and degranulation in basophils, and contributes to the pharmacological activities of marine brown alga, including E. stolonifera.

Inhibitory Effects of Acanthopanax chiisanensis Ethanolic Extracts on FcεRI α Chain Expression

Basophils and mast cells play an important role in FceRI-mediated allergic reaction as effector cells. We studied the effects of Acanthopanax chiisanensis on FceRI α chain expression in human basophilic KU812F cells. Ethanol extracts from root and stem of A. chiisanensis were tested for inhibitory effects of FceRI α chain expression. The cell surface FceRI α chain expression was examined by flow cytometric analysis. All of the extracts of A. chiisanensis reduced the cell surface FceRI α chain expression. Furthermore, A. chiisanensis extracts caused a decrease in the level of FceRI α chain mRNA level and FceRI-mediated histamine release. These results suggest that root and stem extracts of A. chiisanensis play an important role in anti-allergic activity via down-regulation of FceRI α chain expression and decrease in release of inflammatory mediator such as histamine.

Quality Characteristics of Sponge Cake with Omija Powder

We prepared sponge cakes consisting of 1.5%, 3%, 4.5%, or 6% Omija powder. The specific gravity and viscosity of sponge cake batter were measured. Also, the moisture content, color, volume index, weight and texture of the sponge cake were determined. Consumer preference tests of the sponge cakes were also conducted. The viscosity of cake batter tended to decrease as the ratio of Omija powder increased. The specific gravity of the control batter was 0.41, and there was no significant difference between the control and the cakes baked with 1.5%, 3%, 4.5%, and 6% Omija powder. The moisture content and weight of the cakes were not significantly different between the control and those made with Omija powder. Hunter `L,`, `a,` and `b` values of the crust decreased significantly as the amount of Omija powder in the cake increased. Hunter `L` and `b` values of the crumbs were low in the cakes with Omija powder, while `a` values were high. Hardness and fracturability did not show any differences between the cakes. Adhesiveness and resilience of the control were high. The control sample showed the highest sensory score in overall preference. However, cakes made with 1.5% Omija powder obtained the highest values in color, softness, and flavor scores. Omija powder flavor and astringency scores increased as the amount of Omija powder increased, and sweetness did not show any differences among cakes. Intensity scores of egg flavors significantly decreased as the amount of Omija powder increased. Sensory scores of off-flavor significantly increased as the amount of Omija powder increased. Based on the results, 1.5~3% should be recommended as the optimum level of Omija powder to be added for the preparation of sponge cake.

Down-Regulation of FcɛRI Expression by Houttuynia cordata Thunb Extract in Human Basophilic KU812F Cells

Human basophilic KU812F cells express a high-affinity immunoglobulin (Ig) E receptor, FcepsilonRI, which plays an important role in IgE-mediated allergic reactions. Houttuynia cordata Thunb (Family Saururaceae), which is rich in polyphenols, has been shown to have various physiological properties, including antiviral, antioxidative, anticancer, and anti-inflammatory activities. The effect of H. cordata extract on the expression of FcepsilonRI in human KU812F cells was examined. Flow cytometric analysis showed that the FcepsilonRI expression and the IgE binding activity were suppressed when the cells were cultured with H. cordata extract. Reverse transcription-polymerase chain reaction analysis showed that levels of the mRNAs for FcepsilonRI alpha- and gamma-chains were decreased by the treatment of H. cordata extract. Addition of H. cordata extract to culture medium was also observed to result in a reduction in the release of histamine from the cells. These results suggest that H. cordata extract may exert its anti-allergic activity through down-regulation of FcepsilonRI expression and a subsequent decrease in histamine release.

Isolation and identification of flavonoids from Gujeolcho (Chrysanthemum zawadskii var. latilobum) as inhibitor of histamine release

In an ongoing search of compounds from natural sources that exhibit anti-allergic properties, 3 flavonoids were isolated from the ethyl acetatefraction of methanol extracts of gujeolcho (Chrysanthemum zawadskii var. latilobum), with guided histamine release inhibitory effects. The isolated compounds were identified as eriodictyol-7-O-β-d-glucuronide, 5,7-dihydroxy-4-chromene, and 6-methoxyluteolin based on spectroscopic evidences including NMR and MS spectra. These results suggest that gujeolcho extracts and 3 compounds would be candidates as therapeutic or preventive agents for allergic reaction.

Inhibitory Effects of Chrysanthemum zawadsaki Ethanolic Extract on FcεRI α Chain Expression

Department of Food Science and Nutrition, Pukyong National UniversityAbstract Human basophilic KU812F cells express a high affinity for IgE receptor, FceRI, which plays an important rolein immunoglobulin E (IgE)-mediated allergic reactions. Using the human basophilic KU812F cells, we assessed theinhibitory effects of the ethanolic extract from Chrysanthemum zawadsaki(CZ) on FceRI α chain expression. Flowcytometric analysis showed that cell surface FceRI α chain expression was suppressed when the cells were cultured withCZ extract. RT-PCR analysis showed that the mRNAs for FceRI α chain decreased by the treatment of CZ extract.Moreover, CZ extract was also observed to result in a reduction in the release of histamine from anti-FceRI antibody(CRA-1)-stimulated cells. These results suggest that CZ extract may exert anti-allergic activity through down-regulation ofFceRI α chain expression and a subsequent decrease in histamine release.Keywords: Chrysanthemum zawadskii var. latilobum, Fc eRI, histamine