Susan C. Wang
University of Texas at Austin
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Featured researches published by Susan C. Wang.
Biochemistry | 2003
Susan C. Wang; Maria D. Person; William H. Johnson; Christian P. Whitman
Various soil bacteria use 1,3-dichloropropene, a component of the commercially available fumigants Shell D-D and Telone II, as a sole source of carbon and energy. One enzyme involved in the catabolism of 1,3-dichloropropene is trans-3-chloroacrylic acid dehalogenase (CaaD), which converts the trans-isomers of 3-bromo- and 3-chloroacrylate to malonate semialdehyde. Sequence analysis suggested a relationship between the heterohexameric CaaD and the bacterial isomerase, 4-oxalocrotonate tautomerase (4-OT), thereby distinguishing CaaD from a number of dehalogenases whose mechanisms proceed through an alkyl- or aryl-enzyme intermediate. In this study, the genes for the alpha- and beta-subunits of CaaD have been synthesized using a polymerase chain reaction-based strategy, cloned into separate plasmids, and the proteins expressed and purified as the functional heterohexamer. Subsequently, the product of the reaction was confirmed to be malonate semialdehyde by (1)H and (13)C NMR spectroscopy, and kinetic constants were determined using a UV spectrophotometric assay. In view of the proposed hydrolytic nature of the CaaD-catalyzed reaction, three acetylene compounds were investigated as substrates for the enzyme. One compound, 2-oxo-3-pentynoate, a potent active site-directed irreversible inhibitor of 4-OT, is a substrate for CaaD, and was processed to acetopyruvate with kinetic constants similar to those determined for the trans-isomers of 3-bromo- and 3-chloroacrylate. The remaining two compounds, 3-bromo- and 3-chloropropiolic acid, were transformed into potent irreversible inhibitors of CaaD. The inactivation observed for 3-bromopropiolic acid is due to the covalent modification of Pro-1 of the beta-subunit. The results provide evidence for a hydratase activity and set the stage to use the 3-halopropiolic acids as ligands in inactivated CaaD complexes that can be studied by X-ray crystallography.
Bioorganic Chemistry | 2002
Nancy Shine; Susan C. Wang; Krystyna Konopka; Elizabeth A. Burks; Nejat Düzgüneş; Christian P. Whitman
The ability of the salivary protein, secretory leukocyte protease inhibitor (SLPI), to inhibit human immunodeficiency virus-1 (HIV-1) infection in vitro has been reported previously and has led to the suggestion that SLPI may be partially responsible for the low oral transmission rate of HIV-1. However, results contradictory to these findings have also been published. These discrepancies can be attributed to a number of factors ranging from the variability of macrophage susceptibility to HIV infection to the quality of commercially available preparations of SLPI. To resolve these differences and to study further the potential anti-HIV-1 activity of SLPI, the purified and re-folded protein, expressed from a synthetic gene, was examined using human monocytic THP-1 cells. This newly cloned SLPI reduced HIV-1(Ba-L) infection in differentiated THP-1 cells, in contrast to the results observed when using commercially available preparations of SLPI. Interestingly, while the two proteins displayed different anti-HIV effects they had comparable anti-protease activity. The identification of the THP-1 cell line as a system that supports HIV replication, which can be inhibited by a preparation of SLPI now available in large quantities, sets the stage for a thorough investigation of the molecular and structural basis for the anti-HIV activity of SLPI.
Journal of the American Chemical Society | 2003
Susan C. Wang; William H. Johnson; Christian P. Whitman
Biochemistry | 2004
Hugo F. Azurmendi; Susan C. Wang; Michael A. Massiah; Gerrit J. Poelarends; Christian P. Whitman; Albert S. Mildvan
Biochemistry | 2007
Susan C. Wang; William H. Johnson; Robert M. Czerwinski; Stacy L. Stamps; Christian P. Whitman
Biochemistry | 2002
Jeffrey J. Almrud; Andrew D. Kern; Susan C. Wang; Robert M. Czerwinski; William H. Johnson; Alexey Murzin; Marvin L. Hackert; Christian P. Whitman
Biochemistry | 2000
Stacy L. Stamps; Alexander B. Taylor; Susan C. Wang; Marvin L. Hackert; Christian P. Whitman
Biochemistry | 2004
Susan C. Wang; William H. Johnson; Robert M. Czerwinski; Christian P. Whitman
Biochemistry | 2004
William H. Johnson; Susan C. Wang; Thanuja M. Stanley; Robert M. Czerwinski; Jeffrey J. Almrud; Gerrit J. Poelarends; and Alexey G. Murzin; Christian P. Whitman
Bioorganic Chemistry | 2006
Pavel A. Golubkov; William H. Johnson; Robert M. Czerwinski; Maria D. Person; Susan C. Wang; Christian P. Whitman; Marvin L. Hackert
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University of Texas Health Science Center at San Antonio
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