Susie Kim
Catholic University of Korea
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Publication
Featured researches published by Susie Kim.
Journal of Bioscience and Bioengineering | 2011
Yeong-Deok Kwon; Susie Kim; Sang Yup Lee; Pil Kim
To understand the responses of Escherichia coli to high succinate stress and to determine the roles of upregulated genes in high succinate tolerance, a continuous culture of wild-type E. coli W3110 was performed for 268 days in a gradually increasing concentration of succinate. Growth of the final adapted strain, designated DST160, proceeded growth rate of 0.20 h(-1) without a lag phase in medium containing 0.592 M succinate, while the wild-type strain showed 0.02 h(-1) in 38 h. The growth rates of DST160 in media containing either 0.61 M NaCl, 0.61 M KCl, or at pH 4.5 were 25% higher, 18% lower, and 57% higher than those of wild-type, respectively, implying DST160 acquired salt tolerance and pH shock tolerance as well as succinate tolerance. DNA microarray and real-time PCR results indicated that genes controlling active transport and biosynthesis of osmoprotectants were upregulated in DST160 compared to W3110. When ygjE, encoding a putative tartrate/succinate antiporter, and betA, encoding betaine biosynthesis, were expressed in a wild-type E. coli as represent genes for active transport and osmoprotectant synthesis, respectively, greater growth rates were achieved under 0.592 M succinate stress conditions (seven times higher due to ygjE expression and six times higher due to betA expression) than wild-type. The potential to design a metabolic engineering for microbial succinate production is suggested based on the transcriptional regulation of the long-term adapted DST160.
Letters in Applied Microbiology | 2011
Susie Kim; Chung-Hwan Lee; Soo-Wan Nam; Pum-Joon Kim
Aims: To understand the intracellular reducing power metabolism, growth and intracellular NAD(P)H concentrations of a phosphoglucose isomerase (pgi)‐disrupted Escherichia coli (KS002) were investigated with the expressions of redox enzymes.
Journal of Microbiology and Biotechnology | 2009
Oh-Hee Kwon; Susie Kim; Dae-Hyun Hahm; Sang Yup Lee; Pil Kim
Current Microbiology | 2009
Susie Kim; Doo-bum Moon; Chung-Hwan Lee; Soo-Wan Nam; Pil Kim
한국미생물학회 학술대회논문집 | 2008
Oh-Hee Kwon; Susie Kim; Pil Kim
한국미생물학회 학술대회논문집 | 2013
Susie Kim; Pil Kim
한국미생물학회 학술대회논문집 | 2009
Oh-Hee Kwon; Susie Kim; Dae-Hyun Hahm; Hyejung Lee; Pil Kim
한국생물공학회 학술대회 | 2008
Susie Kim; Doo-bum Moon; Pil Kim
한국생물공학회 학술대회 | 2008
Susie Kim; Oh-Hee Kwon; Pil Kim
international conference on systems | 2006
H.U Kim; T.Y Kim; Kwangjoon Jeong; Susie Kim; Joon Haeng Rhee; SangYup Lee