Sylvie Corneille

Conservation of RNA editing between rice and maize plastids: are most editing events dispensable?

Abstract. The extent of conservation of RNA editing sites in the plastid genome of rice was determined by comparing the genomic sequence with that of the cDNA. The presence of a T in the cDNA predicted to be a C by the DNA sequence of the plastid genome, indicated C to U editing. In the 11 plastid transcripts of rice a total of 21 editing sites were found. In maize, a closely related grass species, 26 editing sites have been reported in 13 plastid transcripts. Most editing sites are conserved between the two species, although differences in RNA editing were found at eight sites. In seven cases the T was already encoded at the DNA level, eliminating the requirement for RNA editing. In one case (rpoB, codon 206) the RNA sequence was conserved between the two species, but the mRNA is still not edited in rice. It appears that, although evolutionarily conserved, RNA editing is essential only for a few plastid editing sites. Information about RNA editing in rice plastids will facilitate the design of plastid vectors with broad applicability in grass species.

Tobacco Chloroplasts as a Platform for Vaccine Production

Initial developments in the field of plant-based vaccines have been limited by low level of immunogen expression from nuclear genes. An alternative method is to express vaccine antigens from the chloroplast genome. Recently, we developed a production system for expression of recombinant proteins in tobacco chloroplasts including vectors, expression cassettes, and a system for marker gene elimination. This development is reviewed here. For general reviews on plastid transformation see (Bock, 2001; Maliga, 2002; Staub, 2002).