Tamsin C. O’Connell

From necessity to choice: dietary revolutions in west China in the second millennium BC

Abstract This article explores the context of the long-distance translocation of crops in prehistory. We draw upon contrasts in the isotopic signatures of Southwest Asian crops, including wheat and barley – C3 plants, compared to Asian millets – C4 plants, to investigate a key region of trans-Eurasian exchange, the Chinese province of Gansu. The isotopic results demonstrate that in Gansu province prior to 2000 cal. bc, the staples were millets. Between 2000 and 1800 cal. bc, there was a significant shift in staple foods towards the Southwest Asian crops. In the broader regional context, however, it would seem that these novel crops were not consumed in large quantities in many parts of China during the second millennium bc. This suggests that, while the Southwest Asian crops were adopted and became a staple food source in Gansu province in the second millennium bc, they were disregarded as staple foods elsewhere in the same millennium.

New chronology for Ksâr ‘Akil (Lebanon) supports Levantine route of modern human dispersal into Europe

Significance Bayesian modeling of AMS radiocarbon dates on the marine mollusk Phorcus turbinatus from Ksâr ‘Akil (Lebanon) indicates that the earliest presence of Upper Paleolithic (UP) modern humans in the Levant predates 45,900 cal B.P. Similarities in early UP lithic technology and material culture suggest population dispersals between the Levant and Europe around 50,000–40,000 cal B.P. Our data confirm the presence of modern humans carrying a UP toolkit in the Levant prior to any known European modern human fossils and allow rejection of recent claims that European UP modern humans predate those in the Levant. This result, in turn, suggests the Levant served as a corridor for the dispersal of modern humans out of Africa and into Eurasia. Modern human dispersal into Europe is thought to have occurred with the start of the Upper Paleolithic around 50,000–40,000 y ago. The Levantine corridor hypothesis suggests that modern humans from Africa spread into Europe via the Levant. Ksâr ‘Akil (Lebanon), with its deeply stratified Initial (IUP) and Early (EUP) Upper Paleolithic sequence containing modern human remains, has played an important part in the debate. The latest chronology for the site, based on AMS radiocarbon dates of shell ornaments, suggests that the appearance of the Levantine IUP is later than the start of the first Upper Paleolithic in Europe, thus questioning the Levantine corridor hypothesis. Here we report a series of AMS radiocarbon dates on the marine gastropod Phorcus turbinatus associated with modern human remains and IUP and EUP stone tools from Ksâr ‘Akil. Our results, supported by an evaluation of individual sample integrity, place the EUP layer containing the skeleton known as “Egbert” between 43,200 and 42,900 cal B.P. and the IUP-associated modern human maxilla known as “Ethelruda” before ∼45,900 cal B.P. This chronology is in line with those of other Levantine IUP and EUP sites and demonstrates that the presence of modern humans associated with Upper Paleolithic toolkits in the Levant predates all modern human fossils from Europe. The age of the IUP-associated Ethelruda fossil is significant for the spread of modern humans carrying the IUP into Europe and suggests a rapid initial colonization of Europe by our species.

On the Use of Biomineral Oxygen Isotope Data to Identify Human Migrants in the Archaeological Record: Intra-Sample Variation, Statistical Methods and Geographical Considerations

Oxygen isotope analysis of archaeological skeletal remains is an increasingly popular tool to study past human migrations. It is based on the assumption that human body chemistry preserves the δ18O of precipitation in such a way as to be a useful technique for identifying migrants and, potentially, their homelands. In this study, the first such global survey, we draw on published human tooth enamel and bone bioapatite data to explore the validity of using oxygen isotope analyses to identify migrants in the archaeological record. We use human δ18O results to show that there are large variations in human oxygen isotope values within a population sample. This may relate to physiological factors influencing the preservation of the primary isotope signal, or due to human activities (such as brewing, boiling, stewing, differential access to water sources and so on) causing variation in ingested water and food isotope values. We compare the number of outliers identified using various statistical methods. We determine that the most appropriate method for identifying migrants is dependent on the data but is likely to be the IQR or median absolute deviation from the median under most archaeological circumstances. Finally, through a spatial assessment of the dataset, we show that the degree of overlap in human isotope values from different locations across Europe is such that identifying individuals’ homelands on the basis of oxygen isotope analysis alone is not possible for the regions analysed to date. Oxygen isotope analysis is a valid method for identifying first-generation migrants from an archaeological site when used appropriately, however it is difficult to identify migrants using statistical methods for a sample size of less than c. 25 individuals. In the absence of local previous analyses, each sample should be treated as an individual dataset and statistical techniques can be used to identify migrants, but in most cases pinpointing a specific homeland should not be attempted.

Metals and millets: Bronze and Iron Age diet in inland and coastal Croatia seen through stable isotope analysis

The Bronze and Iron Ages were times of social change throughout Europe, with the development of hillforts and monumental architecture, technological advances and increases in economic specialization and social hierarchy. The extent to which these developments were concurrent with changes in subsistence practices, particularly in the Balkans, is less clear. Croatia provides an opportunity to compare two regions, the inland and coastal zones, with potentially different responses to the social changes through these periods. Here, we present the results of carbon and nitrogen stable isotope analysis of humans from Bronze and Iron Age coastal Croatia and compare these results to the more limited dataset from inland Croatia. The data indicate that in the coastal zone, there was little change in diet between the Bronze and Iron Ages, with perhaps a slight increase in millet consumption or a shift in the environmental carbon isotopic baseline through time. The limited inland dataset, however, suggests that there was a notable increase in millet consumption through these time periods, indicating that in the Iron Age, the inland and coastal zones followed different subsistence strategies. The Iron Age coastal site of Nadin-Gradina provides an opportunity to explore the social value of millet, as individuals buried in simple pits have higher levels of millet consumption than those buried in stone-lined graves, implying that, at this site at least, millet was a low status food.

Climatic or dietary change? Stable isotope analysis of Neolithic–Bronze Age populations from the Upper Ob and Tobol River basins

Dietary changes in the populations inhabiting southwest Siberia and northern Kazakhstan indicate concurrent changes in the economy, at the same time marking the beginnings of East–West interaction across northern Eurasia. The introduction of domestic animal species of Near Eastern origin, such as sheep and goat, dramatically changed the lives of the local population. Past palaeodietary research using stable isotope analysis has mainly focussed on pastoral populations of the Bronze Age period. It is crucial, however, to assess the diets of humans and animals from earlier periods (Neolithic/Chalcolithic) in order to understand the timing and nature of dietary change during the Bronze Age of southwest Siberia and northern Kazakhstan, in particular the possible contribution of environmental change influencing dietary shifts. In this paper, we report the results of stable isotope analysis on 55 human and 45 faunal samples from southwest Siberia (Upper Ob River) and northern Kazakhstan (Tobol River basin), ranging from the Neolithic to the Bronze Age. These data, combined with published human and faunal collagen results from the region as well as new accelerator mass spectrometer (AMS) radiocarbon dating results, indicate little change in animal diet over time, but a notable change in human diet at ca. 2500 cal. BC. The data allow us to determine the time when pastoralism came to the fore, with concomitant economic differences to the local population.

Intraspecific carbon and nitrogen isotopic variability in foxtail millet (Setaria italica)

RATIONALE Isotopic palaeodietary studies generally focus on bone collagen from human and/or animal remains. While plant remains are rarely analysed, it is known that plant isotope values can vary as a result of numerous factors, including soil conditions, the environment and type of plant. The millets were important food crops in prehistoric Eurasia, yet little is known about the isotopic differences within millet species. METHODS Here we compare the stable isotope ratios within and between Setaria italica plants grown in a controlled environment chamber. Using homogenised samples, we compare carbon isotope ratios of leaves and grains, and nitrogen isotope ratios of grains, from 29 accessions of Setaria italica. RESULTS We find significant isotopic variability within single leaves and panicles, and between leaves and panicles within the same plant, which must be considered when undertaking plant isotope studies. We find that the leaves and grains from the different accessions have a ca 2‰ range in δ(13) C values, while the nitrogen isotope values in the grains have a ca 6‰ range. We also find an average offset of 0.9‰ between leaves and grains in their δ(13) C values. CONCLUSIONS The variation found is large enough to have archaeological implications and within- and between-plant isotope variability should be considered in isotope studies. The range in δ(15) N values is particularly significant as it is larger than the typical values quoted for a trophic level enrichment, and as such may lead to erroneous interpretations of the amount of animal protein in human or animal diets. It is therefore necessary to account for the variability in plant stable isotope values during palaeodietary reconstructions. Copyright

Late Pleistocene/Early Holocene Migratory Behavior of Ungulates Using Isotopic Analysis of Tooth Enamel and Its Effects on Forager Mobility

Zooarchaeological and paleoecological investigations have traditionally been unable to reconstruct the ethology of herd animals, which likely had a significant influence on the mobility and subsistence strategies of prehistoric humans. In this paper, we reconstruct the migratory behavior of red deer (Cervus elaphus) and caprids at the Pleistocene-Holocene transition in the northeastern Adriatic region using stable oxygen isotope analysis of tooth enamel. The data show a significant change in δ18O values from the Pleistocene into the Holocene, as well as isotopic variation between taxa, the case study sites, and through time. We then discuss the implications of seasonal faunal availability as determining factors in human mobility patterns.

Ancient human parallel lineages within North America contributed to a coastal expansion

Founder effects in modern populations The genomes of ancient humans can reveal patterns of early human migration (see the Perspective by Achilli et al.). Iceland has a genetically distinct population, despite relatively recent settlement (∼1100 years ago). Ebenesersdóttir et al. examined the genomes of ancient Icelandic people, dating to near the colonization of Iceland, and compared them with modernday Icelandic populations. The ancient DNA revealed that the founders had Gaelic and Norse origins. Genetic drift since the initial settlement has left modern Icelanders with allele frequencies that are distinctive, although still skewed toward those of their Norse founders. Scheib et al. sequenced ancient genomes from the Channel Islands of California, USA, and Ontario, Canada. The ancient Ontario population was similar to other ancient North Americans, as well as to modern Algonquian-speaking Native Americans. In contrast, the California individuals were more like groups that now live in Mexico and South America. It appears that a genetic split and population isolation likely occurred during the Ice Age, but the peoples remixed at a later date. Science, this issue p. 1028, p. 1024; see also p. 964 Two parallel, terminal Pleistocene lineages gave rise to Californian, Central, and South American populations. Little is known regarding the first people to enter the Americas and their genetic legacy. Genomic analysis of the oldest human remains from the Americas showed a direct relationship between a Clovis-related ancestral population and all modern Central and South Americans as well as a deep split separating them from North Americans in Canada. We present 91 ancient human genomes from California and Southwestern Ontario and demonstrate the existence of two distinct ancestries in North America, which possibly split south of the ice sheets. A contribution from both of these ancestral populations is found in all modern Central and South Americans. The proportions of these two ancestries in ancient and modern populations are consistent with a coastal dispersal and multiple admixture events.