Teruo Tanino

Rapid extraction method for detection of outer membrane protein F of Pseudomonas aeruginosa

Abstract A filtration technique has been developed to trap the peptidoglycan sacculus for rapid extraction of the outer membrane protein F of Pseudomonas aeruginosa . The method consists of the following three steps: ( i ) removal of cell components except peptidoglycan associated with some proteins, ( ii ) trapping of peptidoglycan with a glass filter and ( iii ) extraction with a hot SDS solution of proteins, mainly F protein, associated with peptidoglycan. The method is simple and rapid, providing for efficient screening for protein F-deficient mutants of P. aeruginosa . Using this method of screening, three mutants were isolated among 500 mutagenized clones.

The therapeutic efficacy of AC-1370 against experimental infections in mice

The therapeutic efficacy of AC-1370, a new cephalosporin, was compared with that of cefoperazone in experimental infections in mice. For intraperitoneal infections caused by Escherichia coli and Klebsiella pneumoniae, the efficacy of AC-1370 was inferior to that of cefoperazone. On the other hand, AC-1370 was more potent than cefoperazone against pseudomonal infections. The MICs of AC-1370 against the test strains, however, were inferior or equivalent to those of cefoperazone. Serum and peritoneal exudate levels of AC-1370 in infected mice were more durable than those of cefoperazone. The relationship between AC-1370 and neutrophils was investigated. The efficacy of AC-1370 was more dependent on neutrophils than that of cefoperazone. AC-1370 did not enhance the neutrophil functions of migration and phagocytosis to any significant extent.