Thábata dos Anjos Pacheco
Universidade Federal de Mato Grosso
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Medical and Veterinary Entomology | 2016
Andréia Lima Tomé Melo; Rute Witter; Thiago F. Martins; Thábata dos Anjos Pacheco; Alvair S. Alves; Cristiane Silva Chitarra; Valéria Dutra; Luciano Nakazato; Richard C. Pacheco; Marcelo B. Labruna; Daniel Moura de Aguiar
Tick and blood samples collected from domestic dogs in the Brazilian Pantanal were tested by molecular methods for the presence of tick‐borne protozoa and bacteria. Among 320 sampled dogs, 3.13% were infected by Babesia vogeli (Piroplasmida: Babesiidae), 8.75% by Hepatozoon canis (Eucoccidiorida: Hepatozoidae), 7.19% by Anaplasma platys (Rickettsiales: Anaplasmataceae), and 0.94% by an unclassified Anaplasma sp. In three tick species collected from dogs, the following tick‐borne agents were detected: (a) B. vogeli, An. platys and Ehrlichia canis (Rickettsiales: Anaplasmataceae), infecting Rhipicephalus sanguineus sensu lato (Ixodida: Ixodidae) ticks; (b) H. canis, an unclassified Anaplasma sp. and Rickettsia amblyommii (Rickettsiales: Rickettsiaceae), infecting Amblyomma cajennense sensu lato (Ixodida: Ixodidae) ticks, and (c) Rickettsia sp. strain Atlantic rainforest, an emerging human pathogen, infecting Amblyomma ovale ticks. Molecular analysis, based on a mitochondrial gene, revealed that the Am. cajennense s.l. ticks of the present study corresponded to Amblyomma sculptum, a member of the Am. cajennense species complex, and that Rh. sanguineus s.l. belonged to the tropical lineage. Whereas dogs are exposed to a number of tick‐borne bacterial and protozoan agents in the Pantanal biome, humans are potentially exposed to infection by spotted fever group rickettsiae (e.g. R. amblyommii and Rickettsia sp. strain Atlantic rainforest) because both Am. sculptum and Am. ovale are among the most important human‐biting ticks in Brazil.
Ticks and Tick-borne Diseases | 2015
Andréia Lima Tomé Melo; Alvair S. Alves; Fernanda A. Nieri-Bastos; Thiago F. Martins; Rute Witter; Thábata dos Anjos Pacheco; Herbert Sousa Soares; Arlei Marcili; Cristiane Silva Chitarra; Valéria Dutra; Luciano Nakazato; Richard C. Pacheco; Marcelo B. Labruna; Daniel Moura de Aguiar
The present study evaluated the infection of rickettsiae in 151 Rhipicephalus sanguineus, 59 Amblyomma ovale, 166 Amblyomma triste, one Amblyomma dissimile and four Amblyomma dubitatum ticks collected in the municipality of Poconé, State of Mato Grosso, within the Pantanal biome of Brazil. Ticks were individually processed by the hemolymph test with Gimenez staining, isolation of rickettsia in Vero cell culture by the shell vial technique, and polymerase chain reaction (PCR) targeting the citrate synthase rickettsial gene. Through the shell vial technique, rickettsiae were successfully isolated and established in Vero cell culture from one free-living A. triste female tick, which previously showed to contain Rickettsia-like organisms by the hemolymph test. Molecular characterization of the rickettsial isolate was achieved through DNA partial sequences of three rickettsial genes (gltA, ompA, ompB), which showed to be all 100% identical to Rickettsia parkeri. After testing all ticks by PCR, the frequency of R. parkeri infection was 7.23% (12/166) in A. triste adult ticks. The remaining ticks were negative by PCR. This is the first report of in vitro isolation of R. parkeri in the Pantanal biome, confirming the occurrence of this emerging rickettsial pathogen in this natural area of South America.
Revista Brasileira De Parasitologia Veterinaria | 2015
Ricardo Vilas Boas; Thábata dos Anjos Pacheco; Andréia Lima Tomé Melo; Anderson Castro Soares de Oliveira; Daniel Moura de Aguiar; Richard C. Pacheco
Neosporosis is considered a major cause of abortion among cattle worldwide. The aim of this study was to investigate the prevalence of anti-Neospora caninum antibodies in dairy cattle and correlate them with possible risk factors on 63 small farms (family farms) in the municipality of Ji-Paraná, the main milk-producing region of the state of Rondônia, northern Brazil. For this purpose, 621 serum samples were collected from cows and were evaluated by means of the indirect fluorescence antibody test (IFAT). The overall herd prevalence of N. caninum antibodies among the farms (38/63; 60.31%) showed that N. caninum are widespread among the dairy herds in this region, despite only infecting a small proportion of animals (66/621, 10.62%). Occurrences of abortion and birth of weak calves were the only variables that showed as risk factors for the presence of N. caninum. The result from the spatial lag model strongly indicated that birth of weak calves and presence of N. caninum are occurring on farms that are located close to each other, indicating aggregation of disease occurrence.
Revista Brasileira De Parasitologia Veterinaria | 2014
Selma Samiko Miyazaki Onuma; Andréia Lima Tomé Melo; Daniel Luis Zanella Kantek; Peter Gransden Crawshaw-Junior; Ronaldo Gonçalves Morato; Joares Adenílson May-Júnior; Thábata dos Anjos Pacheco; Daniel Moura de Aguiar
Toxoplasma gondii, Neospora caninum and Sarcocystis neurona are related apicomplexan parasites that cause reproductive and neurological disorders in a wide range of domestic and wild animals. In the present study, the immunofluorescence antibody test (IFAT) was used to investigate the presence of antibodies against T. gondii, N. caninum and S. neurona in the sera of 11 free-living jaguars (Panthera onca) in two protected areas in the Pantanal region of Mato Grosso state, Brazil. Ten jaguars (90.9%) showed seropositivity for T. gondii, eight (72.7%) for S. neurona, and seven (63.6%) for N. caninum antigens. Our findings reveal exposure of jaguars to these related coccidian parasites and circulation of these pathogens in this wild ecosystem. To the best of our knowledge, this is the first serological detection of N. caninum and S. neurona in free-living jaguars.
Brazilian Journal of Veterinary Research and Animal Science | 2017
Andréia Lima Tomé Melo; Andréa Pereira da Costa; Selma Samiko Miyazaki; Matias Bassinello Stocco; Thiago Borges Fernandes Semedo; Thábata dos Anjos Pacheco; Rute Witter; Richard C. Pacheco; Marcelo B. Labruna; Arlei Marcili; Daniel Moura de Aguiar
An evaluation was made of the presence of anti- Leishmania infantum chagasi antibodies in domestic dogs from the urban and rural areas of Brazil’s Pantanal wetland region using serological techniques. A total of 429 dogs were sampled in three areas of the Pantanal biome, including the municipalities of Pocone, Santo Antonio de Leverger, and Barao de Melgaco, in the state of Mato Grosso, and in the municipality of Corumba, in the state of Mato Grosso do Sul. The immunofluorescence assay (IFA) was used to detect antibodies (cut-off point 40) using Leishmania infantum chagasi antigen. Because of the possibility of cross-reactivity between species of the genus Leishmania , samples that were positive in the IFA against L. infantum chagasi were also tested by IFA in the same conditions, using L. amazonensis and L. braziliensis antigens. IFA-positive samples to L. infantum chagasi were also evaluated using Enzyme-Linked Immunosorbent Assay (ELISA). The results showed the presence of antibodies against L. infantum chagasi in 23 (5.36%; 95% CI: 3.50%-8.05%) dogs and at least one seroreactive dog was found in each of the municipalities evaluated in this study. Antibody titers ranged from 40 to 5,120, and all IFA positive samples were positive in the ELISA. Among the 23 positive dogs, nine were also were seroreactive for L. amazonensis and L. braziliensis antigens. The occurrence of anti- L. infantum chagasi antibodies in dogs was higher in rural areas (7.06%) than in urban areas (2.50%) (P < 0.05). Based on this study, we concluded that dogs from rural areas of the Pantanal wetlands were in contact with Leishmania species, which is relevant information given their importance to public health.
Ticks and Tick-borne Diseases | 2015
Dirceu Guilherme de Souza Ramos; Andréia Lima Tomé Melo; Thiago F. Martins; Alvair S. Alves; Thábata dos Anjos Pacheco; Letícia B. Pinto; João Batista de Pinho; Marcelo B. Labruna; Valéria Dutra; Daniel Moura de Aguiar; Richard C. Pacheco
Semina-ciencias Agrarias | 2013
Rute Witter; Sarah Nunes Vecchi; Thábata dos Anjos Pacheco; Andréia Lima Tomé Melo; Adriana Borsa; Afonso Lodovico Sinkoc; Adriane Jorge Mendonça; Daniel Moura de Aguiar
Ciencia Rural | 2017
Rute Witter; Andréia Lima Tomé Melo; Thábata dos Anjos Pacheco; Mirella Meneguzzi; Ricardo Vilas Boas; Valéria Dutra; Luciano Nakazato; Cristiane Silva Chitarra; Anderson Castro Soares de Oliveira; Richard C. Pacheco
Semina-ciencias Agrarias | 2016
Robson Souza Rodrigues; Michelle Igarashi; Livia Saab Muraro; Ana Helena Benetti Gomes; Daniel Moura de Aguiar; Thábata dos Anjos Pacheco; Werner Okano; Marcel Pereira Barros; Marcelo Diniz dos Santos
Revista Brasileira De Parasitologia Veterinaria | 2018
Thábata dos Anjos Pacheco; Arlei Marcili; Andréa Pereira da Costa; Rute Witter; Andréia Lima Tomé Melo; Ricardo Vilas Boas; Cristiane Silva Chitarra; Valéria Dutra; Luciano Nakazato; Richard C. Pacheco