Thomas C. Nelson

Cold acclimation increases gene transcription of two calcium transport molecules, calcium transporting ATPase and parvalbumin beta, in carassius auratus lateral musculature

We studied the effects of cold acclimation on gene transcription in goldfish (Carassius auratus) lateral musculature. We incorporated the use of high-density cDNA filter array technology followed by validation of the results with real-time quantitative RT-PCR. Array analysis revealed that the message levels of two calcium transport molecules, calcium transporting ATPase and parvalbumin beta, were higher in cold acclimated fish muscle when compared to warm acclimated fish muscle. Array results indicated that the calcium transporting ATPase message was approximately 8 fold greater in cold acclimated fish muscle, and the parvalbumin beta message was approximately 6 fold greater in cold acclimated fish muscle. The results were confirmed using real-time semi-quantitative RT-PCR. The results are in accordance with known acclimatory response in the calcium transport physiology of Cyprinid muscle.