Tibor Tischner

Frost hardiness depending on carbohydrate changes during cold acclimation in wheat

The effect of cold hardening on the dynamics of frost tolerance and on carbohydrate metabolism was studied in the frost-sensitive Chinese Spring and the frost tolerant Cheyenne genotypes, and in some of the chromosome substitution lines, derived from the crosses of the donor Cheyenne to Chinese Spring. Total water-soluble carbohydrate, glucose, fructose, sucrose and fructan contents were measured in the leaves. Differences in the accumulation of carbohydrates associated with cold tolerance occurred early in response to low temperature. Total water-soluble carbohydrates and total fructan content increased continuously during the cold treatment in all genotypes, resulting in higher contents in tolerant genotypes than in sensitive ones. Their rate of accumulation correlated significantly with the frost tolerance after 19 days of cold treatment. During the cold acclimation, the maximum of fructose accumulation proceeded that of sucrose. Significant correlation was detected between fructose and sucrose content and frost hardiness on the 43rd day of cold treatment. Fructose accumulated to a greater extent in the most tolerant genotypes with a sharp peak on the 35th day of cold hardening, followed by a decrease. In the chromosome substitution lines, the considerable sucrose accumulation started after the 11th day with a maximum on the 43rd day of cold hardening, coinciding with the tolerance test.

Overwintering of winter cereals in Hungary in the case of global warming

Under phytotronic conditions investigations were made on the effect of important environmental factors, such as temperature, water and an increasing concentration of atmospheric CO2, on the hardening of young cereal plants.