Toshitaka Kage

Analysis and identification of human parotid salivary proteins by micro two-dimensional electrophoresis and Western-blot techniques

Using this method, without denaturing agents, over 60 protein components in 2 microliter (about 4 micrograms of protein) of unconcentrated parotid saliva could be detected. After two-dimensional separation, the spots of albumin, secretory IgA, IgG, acid phosphatase, amylase and plasma-originating protein were identified by antibodies with the Western-blot technique.

Two-dimensional electrophoresis in the analysis of a mixture of human sublingual and submandibular salivary proteins

Complex protein mixtures of unstimulated human sublingual and submandibular saliva were fractionated by two-dimensional (2-D) gel electrophoresis, visualized by silver staining and then analysed by immunostaining. Specific proteins were identified by incubation with specific antibody and peroxidase-conjugated second antibody (Western blot). Electrophoresis and silver staining revealed over 50 protein components in 2 microliter of unconcentrated mixture. The Western-blot technique allowed detection of protein spots of plasma origin when an antibody against whole serum was used, but only the albumin spot could be found. Albumin, secretory IgA, acid phosphatase and alpha-amylase were identified with specific antibodies.

Increased β2-microglobulin in both parotid and submandibular/sublingual saliva from patients with Sjögren's syndrome

Abstract Samples of unstimulated saliva from patients with sialoadenopathy were collected by microcapillary tube (1 μl), and their β 2 -microglobulin (B2-MG) content determined by a sandwich enzyme immunoassay. A higher than normal (control) concentration of the globulin was present in both parotid and submandibular/sublingual saliva from the patients with Sjogrens syndrome but not in the samples from the patients with sialoadenitis or diabetes mellitus. The increase in B2-MG in saliva from patients with Sjogrens syndrome may reflect that immunolopathological events are important in the degeneration of both glands in this disease. Therefore, the determination of B2-MG in saliva may be a simple, non-invasive technique for confirming the diagnosis of Sjogrens syndrome as an autoimmune disease.

Two-dimensional electrophoresis of human salivary proteins from patients with sialoadenopathy

Unstimulated saliva was fractionated by micro two-dimensional gel electrophoresis, and the proteins visualized by silver staining and immunostaining. The subjects with Sjögrens syndrome exhibited both quantitative and qualitative alterations in the protein composition of the saliva not only from the parotid gland but also from the submandibular/sublingual glands.

Lung and cervical lymph-node metastasis after cortisone enhancement in hamster cheek-pouch carcinogenesis

The administration of hydrocortisone after excision of DMBA-induced tumours in hamster cheek pouch produced cervical lymph-node metastases in 9 out of 13 animals (69 per cent), and resulted in lung metastasis in 1 animal. In the control group, which received no cortisone but had tumours cut from the pouch, 4 out of 9 animals had cervical lymph-node metastases (44 per cent).

Enhancement by cyclosporin A of metastasis from hamster cheek pouch carcinoma.

Daily intravenous administration of 50 mg/kg cyclosporin A (CsA), after excision of cheek pouch tumors induced with 9,10-dimethyl-1,2-benzanthracene (DMBA), enhanced the incidence of cervical lymph node metastasis to 93%, a significant increase over the rates observed for animals receiving a dosage of 25 mg/kg (43%), and for control animals receiving no CsA (40%). No significant effect was evident on the growth rates of the metastasized tumour cells in the lymph node.

Hyperkeratosis and atypical proliferation appearing in the lining epithelium of a radicular cyst. Report of a case

It is widely accepted that radicular cysts (apical periodontal cysts) are commonly lined with stratified squamous epithelium without keratin formation. However, we identified a case of maxillary radicular cyst with remarkable keratinization and atypical proliferation of the lining epithelium among the 207 radicular cyst cases seen at our department. Histopathological and clinical findings of these cysts were reviewed.

Salivary /gb2-microglobulin in NZB/WFI mice

Abstract Unstimulated parotid and submandibular/sublingual saliva from New Zealand black/WF1 (NZB/WFI) mice as collected by microcapillary (1 μ1), and the content of β 2 -microglobulin ( β 2 -MG) determined by a sandwich enzyme immunoassay. Salivary β 2 -MG contents of control C57BL/6 and NZB/WFI mice (45 weeks of age) were 0.68 ± 0.18 μ g/ml and 1.42 ± 0.21 μ g/ml (mean ± SD), respectively, from the parotid gland and 0.62 ± 0.17 μ g/ml and 1.34 ± 0.2 l μ l/ml, respectively, from the submandibular sublingual glands. However, the concentration of β 2 -MG was not increased in the NZB/WFI mice at 5 and 20 weeks of age. Saliva from NZB/WF1 mice (45 weeks old) was fractionated by micro two-dimensional gel electrophoresis; it exhibited both qualitative and quantitative changes in protein composition in comparison to the two-dimensional at 5 weeks of age. These observations parallel those in saliva from patients with Sjogrens syndrome.