Uğur Zülkadir

Evaluation of ISSR markers for genetic diversity analysis in Anatolian Water Buffaloes

BACKGROUND The utilisation of molecular markers has increased in molecular research recently. Inter-simple sequence repeat (ISSR) markers allow the analysis of genomes without preliminary sequence information, since random primers are used. The purpose of the present study was to evaluate ISSR markers for assessing the genetic diversity of indigenous Anatolian Water Buffaloes reared in Afyon, Konya and Sivas provinces of Turkey, with a view to conservation of the gene resources. RESULTS The 11 ISSR primers chosen for the analysis revealed a total of 110 bands, of which 76 (69.09%) were polymorphic. Also, genetic similarity, polymorphic information content (PIC), resolving power (R(p)) and mean resolving power (R(p)), heterozygosity (H) and Shannon index (I) were calculated as 0.9479-0.9562, 0.35 ± 0.20, 2.73, 0.27, 0.18 ± 0.07 and 0.28 ± 0.11, respectively. CONCLUSION The ISSR markers were found to be promising for assessing the genetic diversity in buffalo populations. Potential genetic parameters such as PIC, R(p), R(p), H and I were effectively used in this study.

Relationship between Persistency Values Defined with Various Methods and Some Lactation Traits of Brown Swiss Cattle in Turkey

Abstract Zülkadir, U., Aytekin, I. and Keskin, 1. 2008. Relationship between persistency values defined with various methods and some lactation traits of Brown Swiss cattle in Turkey. J. App. Arum. Res., 34: 137–141. The study was conducted to compute the persistency values of the animals in different lactation periods with various methods drawing upon 3695 test day milk yield of 182 lactations of Brown Swiss cattle in Konya Konuklar Agricultural Enterprise. The computed persistency values of the animals in the first lactation were different (P<0.01) from the other lactations. Lactation number (P<0.05) and calving seasons (P<0.01) effected persistency values. The phenotypic correlation of the methods within the group and with lactation milk yield, lactation period, daily average milk yield and daily maximum milk yield were highly significant (P<0.01). The Sblkner and Fuchss model based on standard deviation of 305 d lactation period showed the highest phenotypic correlation for lactation milk yield (LMY), daily average milk yield (DAMY), daily maximum milk yield (DMMY), and the best fit to the data collected from Brown Swiss cattle and allowed a suitable description of the shape of the lactation curve.