Umie Lestari

Rambutan peel extract increases leptin efficiency and decreases lipid peroxidation in a rat model of obesity

Obesity induces oxidative stress and causes disturbances in the production of adipokines. Leptin is a hormone synthesized by adipose cells. Rambutan Peel Extract (RPE) contains polyphenols, which can be used as anti-obesity agents. The objective of this study was to analyze the effect of RPE on leptin levels in visceral fat and liver lipid peroxidation in an obese rat model. Thirty male rats aged 12 weeks were divided into groups: normal rat, obese rat model without treatment, obese rat model with 15 mg/kg RPE (O-RPE15), 30 mg/kg (O-RPE30) and 60 mg/kg (O-RPE60). Leptin efficiency, including leptin levels in visceral fat, was detected indirectly by ELISA and food efficiency was determined. Lipid peroxidation parameters, including malondialdehyde (MDA) levels, were determined by the thiobarbituric acid method (TBA), and PPARγ expression in the liver was detected by immunohistochemistry. Visceral fat leptin levels decreased in the O-RPE30 and O-RPE15 groups. Food consumption and calorie intake were decreased in the O-RPE60, O-RPE15 and O-RPE30 groups, respectively. The greatest food efficiency was observed in the O-RPE15 and O-RPE30 treatments. Liver MDA levels decreased in O-RPE15 and O-RPE30 treatments. There were no differences in PPARγ expression in all the treatments.

PENINGKATAN KUALITAS PEMBELAJARAN FISIOLOGI HEWAN DENGAN PENGEMBANGAN BAHAN AJAR BERKARAKTER BERBASIS HASIL PENELITIAN

This study aims to improve the quality of learning by developing character -based teaching materials research animal physiology courses . This research method is the development of module manufacturing as a source of learning based on the research that has been done , including a description, picture research and implementation process that include sites expected character , whether written or unwritten is adapted from Dick and Carey learning system to make some adjustments. The results obtained are based on the calculation and conversion of valuation module by module expert instructional materialsby 71 , 66%. Based on the results of the conversion, the preparation of the module other wise well qualified and do not need to be revised . The results of the assessment calculation and conversion modules by physiologists by 85 %. Based on the results of the conversion, the module qualification other wise excellent preparation and do not need to be revised . Conclusion are obtained t he results by the developing of characterized teaching materials based on the results of experiment is feasible and can be used to support lectures to enhance the quality of teaching Animal Physiology courses in college. Kata kunci: Kualitas pembelajaran fisiologi hewan, bahan ajar berkarakter

Potensi Genistein Terhadap Histopatologi Tubulus Seminiferus Testis Mencit (Mus musculus)

Pemanfaatan sarana kontrasepsi pria dengan memanfaatkan bahan alami berasal dari tanaman sudah banyak dilakukan. Tetapi pemanfaatan biji kedelai sebagai salah satu tanaman suku Leguminoceae belum pernah dilakukan. Biji kedelai mengandung senyawa genistein, sebagai salah satu senyawa derivat isoflavon mempuyai struktur kimia mirip dengan 17I²-estradiol bersifat seperti hormon steroid estrogen, mampu menyebabkan kerusakan pada sistem reproduksi jantan. Penelitian bertujuan menguji pengaruh genistein terhadap histopatologi tubulus seminiferus testis mencit jantan (Mus musculus). Penelitian dilakukan menggunakan pendekatan eksperimental, yang rancangannya mengikuti rancangan acak lengkap. Variabel bebas adalah dosis genistein 0 mg/g, 0,0035 mg/g, 0,0042 mg/g, dan 0,0049 mg/g. Variabel terikat adalah sel-sel germinal tubulus seminiferus testis. Data rerata jumlah sel-sel germinal dianalisis menggunakan Analisis Varians Satu Arah (One Way ANOVA) dengan tingkat signifikansi 95% (I± 5%) dilakukan uji Post Hoc dengan Least Significant Difference (LSD) I± 5%. Perubahan pada jaringan testis dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa ada pengaruh pemberian genistein terhadap rerata jumlah sel-sel germinal dan menyebabkan penghambatan proliferasi sel-sel germinal dalam tubulus seminiferus testis.