Vanderley José Pereira

Concentrações de sais em meios de cultivo para o desenvolvimento de barueiro in vitro

The objective of this work was to evaluate the effect of salt concentrations in culture media on the early development of “barueiro” (Dipteryx alata) seedlings, obtained from the shoot apex, in vitro. Two different in vitro culture media – Murashige & Skoog (MS) and wood plant medium (WPM) – in different salt concentrations (25, 50, 75, and 100%) were used. At 120 days, the D. alata seedlings in the MS medium, in its original concentration (100%), developed better than in other concentrations or in the WPM. Therefore, the MS medium was considered the most suitable for ensuring in vitro establishment because it provided greater root length (27.65 cm) and number of leaves per plant (26 leaves) than the other medium.

Coloração do Fruto e Substrato na Emergência e no Crescimento de Plantas de Eugenia calycina Cambess

O objetivo do trabalho foi avaliar a influencia da coloracao dos frutos na emergencia e no crescimento das plantas de Eugenia calycina Cambess (pitangueira-do-cerrado), assim como de substratos com diferentes proporcoes de material orgânico e estruturante. Tres experimentos foram instalados: o primeiro de emergencia; o segundo de crescimento de plantas constituidos de sementes provenientes de frutos verdes, alaranjados/avermelhados, vermelho-claros e vermelho-escuros; e o terceiro comparou cinco misturas de Bioplant® suplementado com Vermiculita® e com po-de-coco no crescimento das plantas, ambos nas proporcoes de 20% e 40%. A coloracao do fruto nao influenciou a capacidade de emergencia, tempo (inicio, medio e final), velocidade e sincronia de emergencia das plântulas de E. calycina. O crescimento das plantas ate 270 dias de cultivo foi independente da coloracao do fruto. A Vermiculita® e o po-de-coco suplementados ao Bioplant® nao afetaram o crescimento das plantas ate os 120 dias de cultivo.

Coefficient of variation of normal seedlings obtained from the validation of methods for the seed germination testing of 20 species belonging to the family Fabaceae

The standardization of inter-laboratory results of germination test of forest species seeds requires that the methods be robust. Therefore, the objective was to compare and discuss, through the coefficient of variation for normal seedlings, the variabilities present in the process of validation methods obtained in the germination test for seeds of 20 species of the family Fabaceae. Coefficients of variation for the experiment by lot and by laboratory were calculated for normal seedlings from the statistical analysis of method validations. For normal seedlings of 20 Brazilian forest species, the coefficients of variation are low (up to 9.84%), to average (up to 17.66%), contrary to expectations due to high genetic variability in these barely improved species. The increase of the coefficient is not related to treatment for breaking dormancy, but it grows as the lot quality decreases. The high coefficients by laboratory, overestimated by the lot effect, are uniform indicating that the methods are repeatable. The coefficient is not an indicator capable of predicting the heterogeneity of model variance. As normal distribution models random events, randomness is present in the validation process of the 20 forest species of the Fabaceae family.