Vânia Regina Goveia

Microbiological evaluation of different antiseptic povidone-iodine and chlorhexidine formulations after intentional contamination of containers

Os objetivos deste estudo foram: avaliacao da sobrevivencia dos microrganismos nas diferentes formulacoes dos anti-septicos clorexidina (CHX) e polivinilpirrolidona-iodo (PVP-I), apos contaminacao intencional das almotolias, e extrapolar os resultados laboratoriais para o cuidado minimo a ser dispensado as almotolias de multiplo uso para o envase dos anti-septicos testados. Para tanto, foi desenvolvido estudo laboratorial, em que 180 almotolias foram contaminadas com 1 x 105 UFC/mL de suspensao, contendo S.marcescens. Apos a contaminacao, seis diferentes formulacoes de anti-septicos (clorexidina e PVP-I nos veiculos alcoolico, degermante e aquoso) foram distribuidas e submetidas a cultura diaria durante sete dias, a fim de verificar se houve crescimento do microrganismo. Os resultados desta investigacao permitem a recomendacao de que a limpeza como procedimento minimo no processamento desses recipientes garante a seguranca de sua utilizacao repetida para distribuicao dos anti-septicos testados - CHX e PVP-I.This study aimed to evaluate the survival rate of microorganisms within different antiseptic formulations - povidone-iodine (PVP-I) and chlorhexidine (CHX) - after intentional contamination, and to establish the minimum care necessary to ensure sterilization of non-disposable antiseptic solution containers. A laboratory study was performed with 180 antiseptic containers, which were contaminated with Serratia marcescens [1 x 105 UFC/mL]. The containers were closed and stored, at room temperature, during seven days and shaken daily. The antiseptic cultures were evaluated to be 100% negative to Serratia marcescens in all of the non-disposable containers. These results suggested that antiseptic solutions inactivate microorganisms [1 x 105 UFC/mL]. Since cleaned antiseptic containers have around 102 UFC coming from tap water, it can be inferred that cleansing is a safe minimum procedure to ensure reuse of containers for distribution of CHX and PVP-I solutions in aqueous, detergent and alcoholic formulations.

Evaluation of microbial growth on single-use vitrectomy probes reprocessed in healthcare practice

O objetivo deste estudo foi avaliar o crescimento microbiano em sondas para vitrectomia de uso unico, reprocessadas na pratica assistencial. Foram investigadas nove sondas reusadas e reprocessadas por diferentes metodos. As sondas foram segmentadas, individualmente, em porcoes de 3,5 cm, totalizando em 979 unidades amostrais (extensoes, conectores e ponteiras) inoculadas em meio de cultura e incubadas a 37oC, por 14 dias. Os resultados mostraram crescimento microbiano em 57 (5,8%) unidades amostrais, das quais, 25 foram esterilizadas por Oxido de Etileno, 16 por Plasma de Peroxido de Hidrogenio e 16 por Vapor a Baixa Temperatura e Formaldeido. Foram identificadas 17 especies microbianas, sendo as mais prevalentes o Micrococcus spp., Staphylococcus coagulase negativa, Pseudomonas spp. e Bacillus subtilis. O reuso de sondas de uso unico para vitrectomia nao se mostrou seguro, portanto tal pratica nao e recomendada.The aim of this study was to evaluate the microbial growth on single-use vitrectomy probes reprocessed in healthcare practice. We investigated nine vitrectomy probes that had been reused and reprocessed using different methods. The samples were sectioned, individually, in portions of 3.5 cm, totaling 979 sampling units (extensions, connectors and vitrectomy cutters), which were inoculated in culture medium and incubated at 37º C for 14 days. The results showed microbial growth on 57 (5.8%) sample units, 25 of which had been sterilized using ethylene oxide, 16 by hydrogen peroxide plasma, and 16 by low-temperature steam and formaldehyde. Seventeen microbial species were identified. The most prevalent were: Micrococcus spp., coagulase-negative Staphylococcus, Pseudomonas spp., and Bacillus subtilis. The reuse of single-use vitrectomy probes was shown to be unsafe, therefore this practice is not recommended.

Avaliação do crescimento microbiano em sondas de uso único para vitrectomia reprocessadas na prática assistencial

O objetivo deste estudo foi avaliar o crescimento microbiano em sondas para vitrectomia de uso unico, reprocessadas na pratica assistencial. Foram investigadas nove sondas reusadas e reprocessadas por diferentes metodos. As sondas foram segmentadas, individualmente, em porcoes de 3,5 cm, totalizando em 979 unidades amostrais (extensoes, conectores e ponteiras) inoculadas em meio de cultura e incubadas a 37oC, por 14 dias. Os resultados mostraram crescimento microbiano em 57 (5,8%) unidades amostrais, das quais, 25 foram esterilizadas por Oxido de Etileno, 16 por Plasma de Peroxido de Hidrogenio e 16 por Vapor a Baixa Temperatura e Formaldeido. Foram identificadas 17 especies microbianas, sendo as mais prevalentes o Micrococcus spp., Staphylococcus coagulase negativa, Pseudomonas spp. e Bacillus subtilis. O reuso de sondas de uso unico para vitrectomia nao se mostrou seguro, portanto tal pratica nao e recomendada.The aim of this study was to evaluate the microbial growth on single-use vitrectomy probes reprocessed in healthcare practice. We investigated nine vitrectomy probes that had been reused and reprocessed using different methods. The samples were sectioned, individually, in portions of 3.5 cm, totaling 979 sampling units (extensions, connectors and vitrectomy cutters), which were inoculated in culture medium and incubated at 37º C for 14 days. The results showed microbial growth on 57 (5.8%) sample units, 25 of which had been sterilized using ethylene oxide, 16 by hydrogen peroxide plasma, and 16 by low-temperature steam and formaldehyde. Seventeen microbial species were identified. The most prevalent were: Micrococcus spp., coagulase-negative Staphylococcus, Pseudomonas spp., and Bacillus subtilis. The reuse of single-use vitrectomy probes was shown to be unsafe, therefore this practice is not recommended.

Evaluación de crecimiento microbiano en sondas de uso único para vitrectomía recicladas en la práctica asistencial

O objetivo deste estudo foi avaliar o crescimento microbiano em sondas para vitrectomia de uso unico, reprocessadas na pratica assistencial. Foram investigadas nove sondas reusadas e reprocessadas por diferentes metodos. As sondas foram segmentadas, individualmente, em porcoes de 3,5 cm, totalizando em 979 unidades amostrais (extensoes, conectores e ponteiras) inoculadas em meio de cultura e incubadas a 37oC, por 14 dias. Os resultados mostraram crescimento microbiano em 57 (5,8%) unidades amostrais, das quais, 25 foram esterilizadas por Oxido de Etileno, 16 por Plasma de Peroxido de Hidrogenio e 16 por Vapor a Baixa Temperatura e Formaldeido. Foram identificadas 17 especies microbianas, sendo as mais prevalentes o Micrococcus spp., Staphylococcus coagulase negativa, Pseudomonas spp. e Bacillus subtilis. O reuso de sondas de uso unico para vitrectomia nao se mostrou seguro, portanto tal pratica nao e recomendada.The aim of this study was to evaluate the microbial growth on single-use vitrectomy probes reprocessed in healthcare practice. We investigated nine vitrectomy probes that had been reused and reprocessed using different methods. The samples were sectioned, individually, in portions of 3.5 cm, totaling 979 sampling units (extensions, connectors and vitrectomy cutters), which were inoculated in culture medium and incubated at 37º C for 14 days. The results showed microbial growth on 57 (5.8%) sample units, 25 of which had been sterilized using ethylene oxide, 16 by hydrogen peroxide plasma, and 16 by low-temperature steam and formaldehyde. Seventeen microbial species were identified. The most prevalent were: Micrococcus spp., coagulase-negative Staphylococcus, Pseudomonas spp., and Bacillus subtilis. The reuse of single-use vitrectomy probes was shown to be unsafe, therefore this practice is not recommended.