Vinícius Coitinho Tabeleão

Effect of Bacillus cereus var. Toyoi and Saccharomyces boulardii on the immune response of sheep to vaccines

Abstract In this study we evaluated the effects of Bacillus cereus var. Toyoi and Saccharomyces boulardii on the immune response of lambs to Escherichia coli K88ab and Bovine Herpes Virus type 5 (BoHV-5) vaccines. Thirty, 3-month-old lambs were randomly grouped in three lots of 10 each and vaccinated at days 0 and 30 of the experiment. They grazed on the same pasture and were fed ad libitum twice a day with commercial sheep feed supplemented with either B. cereus var. Toyoi at a concentration of 1×106 viable spores gr−1, S. boulardii at a concentration of 1×106 CFU gr−1, or non-supplemented feed. Blood samples were collected at weekly intervals over eight weeks and antibody titres were analysed by ELISA. The mean seroconversions against E. coli and BoHV-5 of the fed probiotics groups were higher (p<0.001) than the controls. Both probiotics enhanced the humoral immune response of lambs to the vaccines.

Combinação de butafosfan e cianocobalamina no metabolismo da glicose em vacas leiteiras após o parto

A hipotese deste estudo e de que o uso da combinacao de butafosfan e cianocobalamina pode melhorar a resistencia periferica a insulina, aumentar a quantidade de glicose disponivel para a glândula mamaria e a producao de leite. Assim, o objetivo foi investigar os efeitos combinados de butafosfan e cianocobalamina sobre o metabolismo da glicose em vacas leiteiras no periodo pos-parto. Vinte e uma vacas leiteiras foram divididas em dois grupos: grupo controle (CON, n= 11), que recebeu cinco aplicacoes de solucao salina (20mL / animal 0,9% NaCl), e grupo Catosal(r) (ABC, n= 10), que recebeu cinco aplicacoes de 20mL de uma solucao contendo as substâncias butafosfan e cianocobalamina (B12 Catosal(r), 100mg da substância butafosfan e 50µg de cianocobalamina por mL). As aplicacoes foram realizadas por via intramuscular, nos dias sete, 12, 17, 22 e 27 pos-parto. As amostras de sangue foram coletadas para a avaliacao das concentracoes plasmaticas de fosforo, glicose, acidos graxos nao esterificados (AGNE), albumina, aspartato aminotransferase (AST) e creatina quinase (CK). Nos dias oito e 28 pos-parto, os animais foram pesados e submetidos aos testes de tolerância a glicose e a insulina. O tratamento promoveu perda de peso (ABC 40,4kg, CON 10,73kg, P<0,05) e aumento da AST (ABC 62,92 ±3,31U/L, CON 53,11±3,49 U / L, P<0,05) e dos niveis de CK (ABC 134,09± 19,08U / L, CON 79,43 ± 18,27U / L). Os grupos nao diferiram quanto ao metabolismo (area sob a curva) da glicose nos dias oito e 28, porem os animais tratados tiveram um aumento na glicemia (P<0,05) no dia 28 pos-parto (97,54 ± 8,54mg / dL), apos a administracao de insulina, em comparacao ao dia oito (83,01 ± 8,54mg / dL). Assim, pode-se concluir que a combinacao de butafosfan e cianocobalamina melhora a adaptacao do metabolismo da glicose em vacas leiteiras no inicio da lactacao.

Efeito da somatotropina recombinante bovina (rbST) sobre os níveis de marcadores metabólicos de touros semiconfinados das raças Aberdeen Angus e Brangus

The aim of this study was to evaluate the effect of recombinant bovine somatotropin (rbST) on metabolic markers for energy, protein, mineral and hepatic metabolism of sires kept grazing and receiving concentrate. Twenty sires Aberdeen Angus and Brangus used for this experiment were divided in two groups (Placebo and rbST). The group Placebo received subcutaneous administration of NaCl, 0.9% and rbST receive rbST twice. Five blood collections with 14 days of interval were made for evaluation of triglyceride and cholesterol as energetic metabolism markers, urea, as proteic marker, alkaline phosphatase and phosphorus as minerals markers and aspartate aminotransferase (AST) and gamma glutamyltransferase (GGT) as indicators of the hepatic function. The rbST did not influence the energetic, mineral and proteic markers, except the hepatic enzyme GGT (P<0,05). These results indicated that the use of rbST in sires did not affect metabolism, therefore evaluated parameters remaining in the physiological range for the specie.