W. Wortmann

Metabolism of 3H-estrone sulfate perfused in vivo through a rhesus monkey liver

Abstract The liver of a rhesus monkey was perfused in vivo with [3H]-estrone sulfate (E1SO4). The injection was made via the portal vein and blood was collected from the hepatic vein, using a heart catheter. Analysis for free and conjugated metabolites was done. The sulfate fraction represented 2 3 of the recovered activity, indicating a slow rate of desulfiirylation. Conversion of estrone sulfate to free estrogens was indicated. 2-hydroxy-estrogens were found in substantial amounts in the conjugate fractions. Double conjugates of estriol and 2-hydroxy-estriol in the form of sulfoglucosiduronates were indicated. Evidence for retention of E1SO4 by the monkey liver was presented.

Determination of specific activity of isotopic materials by thin layer chromatography

A modified method for determination of specific activity by thin layer chromatography (TLC) is described. Based on in situ spectrodensitometry after separation by TLC, the method is rapid and requires amounts of reference material in the microgram range.