Wonho Choe

Flexible thin-layer dielectric barrier discharge plasma treatment of pork butt and beef loin: Effects on pathogen inactivation and meat-quality attributes

The effects of a flexible thin-layer dielectric barrier discharge (DBD) plasma system using a sealed package on microbial inactivation and quality attributes of fresh pork and beef were tested. Following a 10-min treatment, the microbial-load reductions of Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella Typhimurium were 2.04, 2.54, and 2.68 Log CFU/g in pork-butt samples and 1.90, 2.57, and 2.58 Log CFU/g in beef-loin samples, respectively. Colorimetric analysis showed that DBD-plasma treatment did not significantly affect L* values (lightness) of pork and beef samples, but lowered a* values (redness) significantly after 5- and 7.5-min exposures. The plasma treatment significantly influenced lipid oxidation only after a 10-min exposure. The texture of both types of meat was unaffected by plasma treatment. All sensory parameters of treated and non-treated samples were comparable except for taste, which was negatively influenced by the plasma treatment (P < 0.05). This thin-layer DBD-plasma system can be applied to inactivate foodborne pathogens. The observed minor deterioration of meat quality might be prevented by the use of hurdle technology.

The use of atmospheric pressure plasma-treated water as a source of nitrite for emulsion-type sausage.

We investigated the possible use of atmospheric pressure plasma-treated water (PTW) as a nitrite source in curing process. Emulsion-type sausages were manufactured with PTW, celery powder containing nitrite, and synthetic sodium nitrite at a concentration of nitrite ion 70mgkg(-1). In terms of sausage quality, there were no noticeable effects of PTW on the total aerobic bacterial counts, color, and peroxide values of sausages compared with those of celery powder and sodium nitrite throughout 28days of storage at 4°C. Sausage with added PTW had lower concentrations of residual nitrite compared to those of added celery powder and sodium nitrite during the storage period (P<0.05). The sensory properties of PTW-treated and sodium nitrite-treated sausages were not different, whereas the sausage with added celery powder received the lowest scores in taste and acceptability. From the results, it is concluded that PTW can be used as a nitrite source equivalent to a natural curing agent.

Flexible thin-layer plasma inactivation of bacteria and mold survival in beef jerky packaging and its effects on the meat's physicochemical properties

The aims of the present study were to examine the use of a flexible thin-layer plasma system in inactivating bacteria and mold on beef jerky in a commercial package and to evaluate the physicochemical changes of the jerky. After plasma treatment for 10min, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium, and Aspergillus flavus populations on the beef jerky were reduced by approximately 2 to 3Log CFU/g. No significant changes in metmyoglobin content, shear force, and myofibrillar fragmentation index were found in the plasma-treated beef jerky. On the other hand, the peroxide content and L⁎ value were decreased whereas the a⁎ and ΔE value were increased in the plasma-treated sample. Sensory evaluation indicated negative effects of plasma treatment on flavor, off-odor, and overall acceptability of the beef jerky. In conclusion, the flexible thin-layer plasma system could be employed as a means for decontamination of beef jerky, with slight changes to the physicochemical quality of the product.

Intrinsic momentum generation by a combined neoclassical and turbulence mechanism in diverted DIII-D plasma edge

Fluid Reynolds stress from turbulence has usually been considered to be responsible for the anomalous toroidal momentum transport in tokamak plasma. Experiment by Muller et al. [Phys. Rev. Lett. 106, 115001 (2011)], however, reported that neither the observed edge rotation profile nor the inward momentum transport phenomenon at the edge region of an H-mode plasma could be explained by the fluid Reynolds stress measured with reciprocating Langmuir-probe. The full-function gyrokinetic code XGC1 is used to explain, for the first time, Muller et al.s experimental observations. It is discovered that, unlike in the plasma core, the fluid Reynolds stress from turbulence is not sufficient for momentum transport physics in plasma edge. The “turbulent neoclassical” physics arising from the interaction between kinetic neoclassical orbit dynamics and plasma turbulence is key in the tokamak edge region across the plasma pedestal into core.

Evaluation of the microbiological safety, quality changes, and genotoxicity of chicken breast treated with flexible thin-layer dielectric barrier discharge plasma

The microbiological safety, quality changes, and genotoxicity of chicken breasts treated with flexible thin-layer dielectric barrier discharge (FTDBD) plasma inside a sealed package were investigated. Following 10 min plasma treatment, the numbers of total aerobic bacteria, Listeria monocytogenes, Escherichia coli, and Salmonella Typhimurium were reduced by 3.36, 2.14, 2.73, and 2.71 Log CFU/g, respectively. Color L* and b* values increased whereas a* value decreased following plasma treatment with increasing exposure duration. Lipid oxidation was unaffected by plasma treatment. In sensory evaluation, flavor decreased and off-flavor increased with extended plasma exposure time, however, these changes had no effect on acceptability. Increased cohesiveness was the only texture profile analysis parameter that changed following plasma treatment. No genotoxicity was detected in plasma-treated chicken breast using the Salmonella mutagenicity assay. Therefore, FTDBD plasma is applicable since it is able to improve microbiological safety with minimal changes in sensory properties of the chicken breast.

CURRENT STATUS OF NUCLEAR FUSION ENERGY RESEARCH IN KOREA

The history of nuclear fusion research in Korea is rather short compared to that of advanced countries. However, since the mid-1990s, at which time the construction of KSTAR was about to commence, fusion research in Korea has been actively carried out in a wide range of areas, from basic plasma physics to fusion reactor design. The flourishing of fusion research partly owes to the fact that industrial technologies in Korea including those related to the nuclear field have been fully matured, with their quality being highly ranked in the world. Successive pivotal programs such as KSTAR and ITER have provided diverse opportunities to address new scientific and technological problems in fusion as well as to draw young researchers into related fields. The frame of the Korean nuclear fusion program is now changing from a small laboratory scale to a large national agenda. Coordinated strategies from different views and a holistic approach are necessary in order to achieve optimal efficiency and effectiveness. Upon this background, the present paper reflects upon the road taken to arrive at this point and looks ahead at the coming future in nuclear fusion research activities in Korea.

Effect of atmospheric pressure plasma jet on the foodborne pathogens attached to commercial food containers

Bacterial biofilms are associated with numerous infections and problems in the health care and food industries. The aim of this study was to evaluate the bactericidal effect of an atmospheric pressure plasma (APP) jet on Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella Typhimurium biofilm formation on collagen casing (CC), polypropylene (PP) and polyethylene terephthalate (PET), which are widely used food container materials. The samples were treated separately with the APP jet at a 50-W input power for 5 and 10xa0min, and nitrogen (6xa0l per minute) gas combined with oxygen (10 standard cubic centimeters per minute) was used to produce the APP. The APP jet reduced the number of bacterial cells in a time-dependent manner. All pathogens attached to CC, PP, and PET were reduced by 3–4 log CFU/cm2 by the 10-min APP treatment. The developed APP jet was effectively reduced biofilms on CC, PP, and PET.

The creation of electric wind due to the electrohydrodynamic force

Understanding the interactions between ionized matter and neutral particles is a prerequisite for discovering their impact on natural phenomena. One such phenomenon is the electric wind, which supposedly occurs due to the charged particle–neutral coupling in systems of weakly ionized gases, but this mechanism remains unclear. Here, we report direct evidence that electric wind is caused by an electrohydrodynamic force generated by the charged particle drag as a result of the momentum transfer from electrons/ions to neutrals. The model experiment is based on a pulsed plasma jet as a source of weakly ionized gases generated in the helium gas at atmospheric pressure using Schlieren photography. Studying the helium gas flow trajectories at different discharge parameters allows one to distinguish between the effects of streamer propagation or space charge drift causing the electric wind as well as to determine the role of electrons and (positive) ions in wind generation.The electric wind mechanism remains unclear. Here, the authors report evidence that electric wind is caused by an electrohydrodynamic force generated by charged particle drag as a result of momentum transfer to neutral particles.

Spatio-temporally resolved electron temperature in argon radio-frequency capacitive discharge at atmospheric pressure

Due to the lack of convincing experimental evidence for electron information, there are still unclearly understood discharge phenomena in atmospheric pressure radio-frequency (rf) capacitive discharge, e.g. the electron heating, discharge structures, and the alpha–gamma mode transition. Thus, to perceive basic and meaningful principles with an unambiguous interpretation, simple and reliable electron diagnostics are required. Since bremsstrahlung emitted through electron-neutral atom interaction depends on electron density (ne) and temperature (Te), their diagnostic is possible. In particular, Te is easily estimated from the ratio of bremsstrahlung emissivities at two different wavelengths or more. In this paper, 2D Te distribution in an argon atmospheric pressure capacitive discharge measured by using a digital camera and optical band pass filters is described. Time-averaged Te in the bulk region obtained by a digital camera is consistent with that measured by an absolutely calibrated spectrometer. In addition, time-resolved emission spectra and the corresponding ne and Te during one rf cycle of the argon capacitive discharge are discussed. The result shows that Te varied from 2.3 to 3.0 eV, while ne did not change significantly.

Inactivation of murine norovirus-1 and hepatitis A virus on fresh meats by atmospheric pressure plasma jets

In the current study, inactivation effect of atmospheric pressure plasma (APP) jets (10s-20min) was investigated against murine norovirus (MNV-1), as a norovirus (NoV) surrogate and hepatitis A virus (HAV) associated with three types of fresh meats (beef loin, pork shoulder and chicken breast). The quality characteristics of fresh meats, such as surface color, moisture content and thiobarbituric acid reactive substance (TBARS) were also examined. After 5-20min of treatment with APP jets, the reduction in MNV-1 titers (initial inoculums of 107 plaque-forming units (PFU)) were >2log10PFU/mL in the three types of meat. After 5-20min treatment with APP jets, the reduction in HAV titers (initial inoculums of 106PFU) were >1log10PFU/mL in the three types of meat. There was no significant difference (p>0.05) in the L*, a*, and b* values for APP jet treatment times below 5min. Furthermore, there was no significant difference (p>0.05) in the water content (%) value for treatment times under 5min. Although the TBARS values gradually increased with increase in APP jet treatment times, these TBA values were below 1.0mgMA/kg (an indicator of meat rancidity). The results of the current study indicate that 5min of APP jet treatment showed >99% reduction (2log10PFU/mL) of MNV-1 titer and >90% reduction (1log10PFU/mL) of HAV titer without concomitant changes in meat quality; thus, this procedure can be considered in fresh meat production, processing and distribution processes to enhance fresh meat safety.