Y.L. Zhang

Effects of isobutyrate supplementation on ruminal microflora, rumen enzyme activities and methane emissions in Simmental steers

The objective of this study was to evaluate the effects of isobutyrate supplementation on rumen microflora, enzyme activities and methane emissions in Simmental steers consuming a corn stover-based diet. Eight ruminally cannulated Simmental steers were used in a replicated 4 × 4 Latin square experiment. The treatments were control (without isobutyrate), low isobutyrate (LIB), moderate isobutyrate (MIB) and high isobutyrate (HIB) with 8.4, 16.8 and 25.2 g isobutyrate per steer per day respectively. Isobutyrate was hand-mixed into the concentrate portion. Diet consisted of 60% corn stover and 40% concentrate [dry matter (DM) basis]. Dry matter intake (averaged 9 kg/day) was restricted to a maximum of 90% of ad libitum intake. Population of total bacteria, cellulolytic bacteria and anaerobic fungi were linearly increased, whereas that of protozoa and total methanogens was linearly reduced with increasing isobutyrate supplementation. Real-time PCR quantification of population of Ruminococcus albus, Ruminococcus flavefaciens, Butyrivibrio fibrisolvens and Fibrobacter succinogenes was linearly increased with increasing isobutyrate supplementation. Activities of carboxymethyl cellulase, xylanase and β-glucosidase were linearly increased, whereas that of protease was linearly reduced. Methane production was linearly decreased with increasing isobutyrate supplementation. Effective degradabilities of cellulose and hemicellulose of corn stover were linearly increased, whereas that of crude protein in diet was linearly decreased with increasing isobutyrate supplementation. The present results indicate that isobutyrate supplemented improved microflora, rumen enzyme activities and methane emissions in steers. It was suggested that the isobutyrate stimulated the digestive micro-organisms or enzymes in a dose-dependent manner. In the experimental conditions of this trial, the optimum isobutyrate dose was approximately 16.8 g isobutyrate per steer per day.

Effects of substituting corn with steam-flaked sorghum on growth, digestion and blood metabolites in young cattle fed feedlot diets

The objective of the present study was to evaluate the effects of substituting ground corn grain with steam-flaked sorghum (SFS) grain on growth performance, nutrient utilisation and blood metabolites in beef bulls. Fifty-six Simmental beef bulls averaging 12 months of age and 356.4 ± 2.6 kg of bodyweight were randomly assigned to four groups. The treatments were control, low-SFS, medium-SFS and high–SFS, with 0, 1/3, 2/3 and 3/3 SFS grain, respectively, substituting ground corn grain of diets(dry matter (DM) basis). Diets consisted of 50% corn silage and 50% concentrate (DM basis). The average daily gain and feed conversion rate improved quadratically with increasing the proportion of SFS grain. Ruminal pH and ammonia-nitrogen (N) concentration decreased quadratically, whereas ruminal total volatile fatty acid concentration increased quadratically. Ratio of acetate to propionate decreased quadratically (P = 0.027) due to the quadratic decrease in acetate production and the quadratic increase in propionate production. Digestibilities of DM, organic matter and crude protein increased quadratically, whereas that of neutral detergent fibre and acid detergent fibre decreased quadratically. The digestible N, retention N and the retention N to digestible N ratio improved quadratically. Inclusion of SFS grain in diets quadratically increased blood concentrations of glucose, total protein, albumin and triglyceride, but quadratically decreased the concentration of urea N. The results indicated that partly substituting ground corn grain with SFS grain could improve growth performance by improving ruminal fermentation and N utilisation; the optimum substitution rate was 2/3 (DM basis).

Effects of fibrolytic enzymes and isobutyrate on ruminal fermentation, microbial enzyme activity and cellulolytic bacteria in pre- and post-weaning dairy calves

The objective of the present study was to evaluate the effects of fibrolytic enzymes (FE, containing 160 units of cellulase and 4000 units of xylanase) or isobutyrate (IB) supplementation on ruminal fermentation, microbial enzyme activity and cellulolytic bacteria in dairy calves. Forty-eight Holstein bull calves of 15 days of age and of 44.9 ± 0.28 kg of BW were randomly assigned to four groups in a 2 × 2 factorial arrangement. Two levels of FE (0 g (FE–) or 1.83 g per calf per day (FE+)) and IB (0 g (IB–) or 6 g per calf per day (IB+)) were added. Calves were weaned at 60-day-old and four calves were selected from each treatment at random and slaughtered at 45 and 90 days of age. There was no IB × FE interaction effect. Ruminal pH decreased with IB or FE supplementation for post-weaned calves, whereas concentrations of total volatile fatty acids and acetate increased with IB or FE supplementation for pre- and post-weaned calves. Acetate to propionate ratio increased with IB supplementation, but was unaffected by FE supplementation. Ammonia-N concentration decreased with IB or FE supplementation for pre- and post-weaned calves. For post-weaned calves, activities of CMCase increased with IB or FE supplementation, and activities of cellobiase, xylanase, pectinase, β-amylase and protease increased with IB supplementation. Populations of B. fibrisolvens and F. succinogenes for pre- and post-weaned calves and R. flavefaciens for post-weaned calves increased with IB or FE supplementation. It is suggested that ruminal fermentation and growth performance of calves was improved with IB and FE supplementation, and the combination of IB and FE has the potential to stimulate the growth of pre- and post-weaned dairy calves.