Ya-Li Shiu
National Pingtung University of Science and Technology
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Ya-Li Shiu.
Fish & Shellfish Immunology | 2009
Deng-Yu Tseng; Pei-Lin Ho; Sung-Yan Huang; Sheng-Chi Cheng; Ya-Li Shiu; Chiu-Shia Chiu; Chun-Hung Liu
Effects of Bacillus subtilis E20 isolated from fermented soybean on immune parameters and the disease resistance of the white shrimp (Litopenaeus vannamei) after 98 days of B. subtilis E20 feeding were evaluated in this study. Shrimp fed B. subtilis E20-containing diets at concentrations of 10(6) (E206), 10(7) (E207), and 10(8) (E208)cfu kg(-1), respectively, had significantly increased survival rates of 13.3%, 16.7%, and 20%, compared to the control (fed no probiotic) after being challenged with Vibrio alginolyticus. There were no significant differences in the total hemocyte count, respiratory burst, or superoxide dismutase glutathione peroxidase among all treatments. Shrimp fed a higher concentration of the probiotic (E208) exhibited significant increases in phenoloxidase activity, phagocytic activity, and clearance efficiency compared to control shrimp. In addition, B. subtilis E20 showed a weaker inhibitory effect against the growth of Aeromona hydrophila with around a 0.3-cm inhibitory zone, but showed no inhibitory effects against other selected pathogens, such as white shrimp pathogens: V. alginolyticus and Vibrio vulnificus. These results suggest that the increased resistance of shrimp after B. subtilis E20 consumption occurs through immune modifications, such as increases in phenoloxidase activity, phagocytic activity, and clearance efficiency against V. alginolyticus.
Fish & Shellfish Immunology | 2010
Kuan-Fu Liu; Chiu-Hsia Chiu; Ya-Li Shiu; Winton Cheng; Chun-Hung Liu
In this study, the probiotic, Bacillus subtilis E20, isolated from the human health food, natto, was used for white shrimp, Litopenaeus vannamei, larvae breeding to improve the larval survival rate and development by adding probiotic to the rearing water at (control), 10(8), and 10(9) cfu L(-1) salt water once every 3 days during the 14 days of breeding experiment. Thereafter, stress tolerance and immune status of postlarvae were evaluated. Shrimp larval development was significantly accelerated after adding the probiotic to the larval rearing water at a level of 10(9) cfu L(-1). The survival rate of larvae was significantly higher in the treatment with 10(9) cfu L(-1) compared to the control and the treatment with 10(8) cfu L(-1) after all larvae had metamorphosed to postlarvae. Adding the probiotic to the shrimp larvae rearing water produced a weak inhibition of bacterial growth by an analysis of the total bacterial count and presumptive Vibrio count. For stress tests, no postlarvae died when they were reared in water in which the temperature was decreased from 30 to 2 degrees C at a rate of 0.1 degrees C min(-1). Postlarvae had significantly lower cumulate mortality in the treatments with 10(8) and 10(9) cfu L(-1) compared to the control when they were suddenly exposed to fresh water and 60 per thousand salt water. A significant decrease in the cumulative mortality of postlarvae treated with the probiotic at a level of 10(9) cfu L(-1) was recorded after the sudden transfer to 300 mg L(-1) nitrite-N compared to the control and treatment with 10(8) cfu L(-1). The analysis of immune-related gene expressions showed that the gene expression of prophenoloxidase I, prophenoloxidase II, and lysozyme of larvae were significantly increased after being reared in probiotic-containing water at the levels of 10(8) and 10(9) cfu L(-1). However, no significant difference in serine proteinase or glutathione peroxidase gene expressions was recorded in this study. It is therefore suggested that 10(9) cfu L(-1) of probiotic, B. subtilis E20 adding to rearing water for shrimp larva breeding.
Fish & Shellfish Immunology | 2009
Ruo-Yun Yeh; Ya-Li Shiu; Shu-Chiu Shei; Sheng-Chi Cheng; Sung-Yan Huang; Jiunn-Cheng Lin; Chun-Hung Liu
Effects of essential oils and hot-water extracts isolated from leaf and twig of stout camphor tree, Cinnamomum kanehirae on antibacterial activity to pathogen of fish, abalone, marine fish and freshwater prawn, and the white shrimp, Litopenaeus vannamei immunity and disease resistance to Vibrio alginolyticus were carried out in this study. A better antibacterial activity against nine selected pathogen bacteria was recorded in twig essential oil, and the selected pathogens of both Gram-positive bacteria and Gram-negative bacteria were sensitive to the leaf and twig essential oils in the present study. No antibacterial activity was recorded in the hot-water extracts of leaf and twig. In challenge trial, a significant decrease of sensitivity to V. alginolyticus (1 x 10(6) cfu shrimp(-1)) was found in that of shrimp received hot-water extract from twig at the levels of 2 microg g shrimp(-1) compared to control. In addition, the how-water extract of twig in vitro showed greater enhanced effects on phenoloxidase activity, respiratory burst and phagocytosis of white shrimp compared to the hot-water extract of leaf. It is considered that the extracts of stout camphor tree could be a candidate to replace the chemo-therapeutants through the inhibitory effects against the growth of pathogens, and enhanced effects on shrimp immunity and disease resistance.
Fish & Shellfish Immunology | 2017
Truong-Giang Huynh; Ya-Li Shiu; Thanh-Phuong Nguyen; Quoc-Phu Truong; Jiann-Chu Chen; Chun-Hung Liu
Synbiotics, a conjunction between prebiotics and probiotics, have been used in aquaculture for over 10 years. However, the mechanisms of how synbiotics work as growth and immunity promoters are far from being unraveled. Here, we show that a prebiotic as part of a synbiotic is hydrolyzed to mono- or disaccharides as the sole carbon source with diverse mechanisms, thereby increasing biomass and colonization that is established by specific crosstalk between probiotic bacteria and the surface of intestinal epithelial cells of the host. Synbiotics may indirectly and directly promote the growth of aquatic animals through releasing extracellular bacterial enzymes and bioactive products from synbiotic metabolic processes. These compounds may activate precursors of digestive enzymes of the host and augment the nutritional absorptive ability that contributes to the efficacy of food utilization. In fish immune systems, synbiotics cause intestinal epithelial cells to secrete cytokines which modulate immune functional cells as of dendritic cells, T cells, and B cells, and induce the ability of lipopolysaccharides to trigger tumor necrosis factor-α and Toll-like receptor 2 gene transcription leading to increased respiratory burst activity, phagocytosis, and nitric oxide production. In shellfish, synbiotics stimulate the proliferation and degranulation of hemocytes of shrimp due to the presence of bacterial cell walls. Pathogen-associated molecular patterns are subsequently recognized and bound by specific pattern-recognition proteins, triggering melanization and phagocytosis processes.
Fish & Shellfish Immunology | 2017
Hsueh-Li Lin; Ya-Li Shiu; Chiu-Shia Chiu; Shih-Ling Huang; Chun-Hung Liu
ABSTRACT Six bacteria, including, Lactobacillus casei M15, Lac. plantarum D8, Lac. pentosus BD6, Lac. fermentum LW2, Enterococcus faecium 10–10, and Bacillus subtilis E20, and one yeast, Saccharomyces cerevisiae P13 were selected as probiotics for Asian seabass, Lates calcarifer, by tracking the growth performance and disease resistance of fish against Aeromonas hydrophila in the first trial. The probiotic efficiency screening results showed that B. subtilis E20 and Lac. pentosus BD6, and S. cerevisiae P13 and Lac. fermentum LW2 respectively improved either the growth performance or disease resistance. Therefore, these four probiotics were then selected to prepare a probiotics mixture, and this was incorporated in equal proportions into diets for Asian seabass at levels of 0 (control), and 106 (MD6), 107 (MD7), 108 (MD8), and 109 (MD9) colony‐forming units (cfu) (kg diet)−1. A synergistic effect of the combined probiotics was investigated in this study, and the probiotics mixture was able to improve both the growth performance and health status of fish. After 56 days of feeding, fish fed the MD9 diet had a higher final weight and percentage of weight gain. In addition, protein contents in the dorsal muscle of fish fed the MD8 and MD9 diets were significantly higher compared to the control. For the pathogen challenge test, fish fed the MD7, MD8, and MD9 diets had significantly lower cumulative mortalities after A. hydrophila infection compared to those of fish fed the control and MD6 diets, which might have been due to increased respiratory bursts, decreased superoxide dismutase activity in leucocytes, and increased phagocytic activity. Therefore, we considered that the probiotics mixture could adequately provide probiotic efficiency for Asian seabass, and the diet containing 109 cfu (kg diet)−1 probiotic mixture is recommended to improve the growth and health status of Asian seabass. HIGHLIGHTSBacillus subtilis E20 and Lactobacillus pentosus BD6 are potential probiotics for promoting the growth of the Asian seabass, Lates calcarifer.Saccharomyces cerevisiae P13 and Lac. fermentum LW2 are potential probiotics for improving the health status of L. calcarifer.A synergistic effect of combined probiotics containing E20, BD6, P13, and LW2 was observed to improve both the growth performance and health status of fish in this study.A high dose (109 cfu (kg diet)−1) of the probiotic mixture is recommended to improve the growth performance and health status of L. calcarifer.
Fish & Shellfish Immunology | 2017
Ann-Chang Cheng; Hsueh-Li Lin; Ya-Li Shiu; Yu-Chang Tyan; Chun-Hung Liu
Abstract Bacillus subtilis E20‐fermented soybean meal (FSBM) was found to produce antimicrobial peptides (AMPs) with great antimicrobial activity against Vibrio alginolyticus (VA) and V. parahaemolyticus (VP). Three AMPs were purified with a 5 kDa ultrafiltration, Sephadex G‐15 column and reverse‐phase high‐performance liquid chromatography (RP‐HPLC). The FSB‐AMP, HTSKALLDMLKRLGK, identified by an RP‐nano‐ultrapure liquid chromatography (UPLC) electrospray ionization (ESI)‐tandem mass spectroscopic (MS/MS) analysis exhibited the highest bactericidal activity against VA and VP compared to the others. The antimicrobial activity assessment indicated that FSB‐AMP inhibited the growth of VA and VP with minimal inhibitory concentrations of 72.5 and 72.5 &mgr;M. Alterations in the morphology of VA were observed by scanning electronic microscopy, and membrane disruption of VA and VP was confirmed by fluorescent microscopy with propidium iodide staining. The FSB‐AMP was then incorporated into the diet of white shrimp, Litopenaeus vannamei, and a protective effect in shrimp against VP infection was recorded as well as for shrimp fed a diet containing 15% fish meal replaced by B. subtilis E20‐FSBM. Results demonstrated that B. subtilis E20‐FSBM could be a biofunctional ingredient to prevent vibriosis in shrimp aquaculture. HighlightsBacillus subtilis E20‐fermented soybean meal (FSBM) exhibited good bactericidal activity against Vibrio alginolyticus (VA) and V. parahaemolyticus (VP).FSB‐AMP inhibited Vibrio growth through interaction with bacterial membranes which caused an increase in their permeability.FSB‐AMP and B. subtilis E20‐FSBM incorporated into diets of white shrimp could reduce infection by V. parahaemolyticus.FSBM increased the intestine immune function of L. vannamei.
Fish & Shellfish Immunology | 2015
Shieh-Tsung Chiu; Ya-Li Shiu; Tsung-Meng Wu; Yu-Syuan Lin; Chun-Hung Liu
A 42-day study was conducted with barramundi, Lates calcarifer, to evaluate the effects of Daphnia meal derived from Daphnia similis on fish growth, immune response, and disease resistance to Aeromonas hydrophila. Three isonitrogenous (45%) and isolipid (10%) experimental diets were formulated to contain 0% (control), 5% (D5), and 10% (D10) Daphnia meal. Growth was depressed when fish were fed with the D10 diet for 42 days compared to control. However, the growth in fish fed with control and D5 diets for 42 days was not significantly different. By day 42, the leukocyte phagocytic activity and respiratory burst activity were significantly increased in D5 and D10 groups compared to control. Mx gene expression in the spleen and head kidney of fish after being injected with nerve necrosis virus was also significantly up-regulated in both groups compared to control. In an increased immune response, D5 and D10 fish had significantly higher survival rates than control after being challenged by A. hydrophila. Therefore, we suggest that a 5% Daphnia-meal diet could improve the barramundi immune response and disease resistance without a negative impact on growth.
Fish & Shellfish Immunology | 2017
Ya-Li Shiu; Kuo-Hsun Chiu; Truong-Giang Huynh; Ping-Chung Liu; Chun-Hung Liu
&NA; This study aimed to unravel the regulatory roles of choline in activating immune responses and disease resistance of the orange‐spotted grouper Epinephelus coioides. Fish were fed a choline‐supplemented diet at 1 g kg−1 of feed for 30 days. Fish fed a fish meal basal diet without choline‐supplement served as controls. At the end of the feeding trial, fish were challenged with Vibrio alginolyticus. Meanwhile, plasma proteomics of fish in each group were also evaluated by two‐dimensional gel electrophoresis (2‐DE), and differentially expressed proteins were identified by tandem mass spectrophotometry (MS/MS), then a Western blot analysis or real‐time polymerase chain reaction was used to confirm differential expressions of immune‐enhancing proteins. Results showed that choline significantly increased survival of E. coioides 48 days after being injected with V. alginolyticus. From maps of plasma proteins, a comparative analysis between the control and choline groups revealed that 111 spots matched, with 26 altered expression spots in the choline group. Of these 26 spots, 16 were upregulated and 10 downregulated. After protein identification by reverse‐phase nano‐high‐performance liquid chromatography‐electrospray ionization MS/MS analysis, eight of 26 proteins were found to be immune‐related proteins, all of which were upregulated, including complement 3 (C3), alpha‐2‐macroglobulin‐P‐like isoform (A2M), fibrinogen beta chain precursor (FBG), and immunoglobulin heavy constant mu (Ighm) proteins. Expression of the A2M protein and A2M enzyme activity in plasma of fish fed choline significantly increased compared to the control group. Additionally, A2M messenger (m)RNA transcripts were also upregulated in the liver and kidneys. Significantly higher C3 expressions at both the mRNA and protein levels were detected in the liver of fish in the choline group. Moreover, FBG gene expressions in the liver and kidneys significantly increased, while Ighm increased in the kidneys and spleen of fish in the choline group. Our results suggest that dietary administration of choline can protect grouper against bacterial infections through activating the complement system, thereby inducing antiprotease activity and natural antibodies that play important roles in the innate immune system of fish. HighlightsDietary choline improve orange‐spotted grouper immunity and disease resistance against Vibrio alginolyticus infection.Four upregulated immune‐protein were identified proteomically in plasma of grouper.Differentially expressed protein indicated that dietary choline promoted the non‐specific and specific immune responses.
臺灣水產學會刊 | 2011
Shinn-Pyng Yeh; Ya-Li Shiu; Shieh-Tsung Chiu; Jung-Ping Hsu; Winton Cheng; Chun-Hung Liu
Differences in osmolality, ion concentrations, and respiratory metabolism of the hemolymph were investigated in terminal-growth male and other morphotypes of healthy prawn, including males of orangeclaw and blue-claw, and females of the giant freshwater prawn, Macrobrachium rosenbergii. In addition, gill-injured prawns were also used to analyze those same parameters in this study. No significant differences in hemolymph osmolality, ion levels, or respiratory metabolism among blue-claw males, orange-claw males, and females were found. Hemolymph osmolality, chloride, potassium, sodium, calcium, and magnesium concentrations in terminal-growth males were significant lower than in other types of prawn. Significantly lower oxyhemocyanin and protein, and significantly higher hemolymph pH and PCO2 in terminal-growth males were found compared to other types of prawn. Insignificant differences in the ratio of oxyhemocyanin and protein, and in hemolymph PO2 among terminal-growth males and other types of prawn were recorded. Histopathological lesions in gills of terminal-growth males caused by an Epistylis sp. infestation and impurities covering the gills were found, that may have involved interference with gas exchange, hemolymph osmolality, and ion regulation. Prawns with partially injured gills had significantly decreased regulation of osmolality and ions in the hemolymph, but no significant change in respiratory metabolism compared to control prawn. Prawn gills are considered the main tissuefor hemolymph osmolality and ion regulation because hemolymph osmolality and ion levels of prawn decreased when gills of prawn were partially injured. However, respiratory metabolism was compensated for by normal gills in prawns with partially injured gills. The information obtained from this study adds to knowledge of prawn pathophysiology and may give a clearer profile of changes in hemolymph osmolality, ion concentrations, and respiratory metabolism caused by gill injury.
Journal of the Fisheries Society of Taiwan | 2010
Chun-Hung Liu; Chi-Tai Lee; Ya-Li Shiu; Winton Cheng; Shinn-Pyng Yeh
The color metamorphosis, growth performance, food intake, total harvest weight and survival rate of a juvenile hybrid ornamental fish, blood parrotfish fish (Cichlasoma citrinellum × Cichlasoma synspilum) was studied at three different temperature, 18oC, the temperature of natural variation (24.4-28.7℃) and 32℃ for 84 days, respectively. The results showed that growth rate of blood parrotfish reared at 32℃ was significant higher than that of blood parrotfish reared fish at 18℃ and natural temperature variation, resulted from the increase of food intake accompanying a decrease of feed conversion ratio. In addition, an adverse temperature of 18℃ results in an insignificant growth and no color metamorphosis in blood parrotfish during 84 days of culture. Results of the present study strongly suggested that better rearing temperature of blood parrotfish is 32℃.