Yancheng Yao
University of Giessen
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Featured researches published by Yancheng Yao.
Veterinary Microbiology | 2017
Linda Falgenhauer; Hiren Ghosh; Beatriz Guerra; Yancheng Yao; Moritz Fritzenwanker; Jennie Fischer; Reiner Helmuth; Can Imirzalioglu; Trinad Chakraborty
Carbapenem-resistant Enterobacteriaceae are not any more isolated only from human settings, but also from livestock. We reported for the first time the presence of VIM-1 carbapenemases in a livestock farm in Germany. The VIM-1 resistance gene found in these farms was located on IncHI2 plasmids. In order to be able to analyse these plasmids in more detail, two different plasmids from a single farm (pRH-R27 from Salmonella enterica and pRH-R178 from Escherichia coli) were completely sequenced and analysed for the presence of antibiotic and heavy metal resistances. The plasmids showed to harbour blaVIM-1, aacA4, aadA1, sul1, qacEΔ (encoded in an In110 class 1 integron), as well as blaACC-1, strA/strB, and catA1 genes together with resistance to heavy metals (ter-, mer-, sil-, ars-, rcn-, and pco). Comparison with other IncHI2 plasmid revealed that while pRH-R27 is a mosaic IncHI2 plasmid with both high homology to the plasmid pSTm-A54650 and R478, both isolated from humans, pRH-R178 is a deletion derivative of pRH-R27, presumably caused by several IS-mediated deletions indicating genetic evolution of plasmids in this environment.
Genome Announcements | 2014
Linda Falgenhauer; Yancheng Yao; Moritz Fritzenwanker; Judith Schmiedel; Can Imirzalioglu; Trinad Chakraborty
ABSTRACT A nonconjugative and nontypable plasmid of a clinical Escherichia coli isolate expressing resistance to extended-spectrum cephalosporins (ESCs) was isolated and sequenced. The plasmid pECOH89 contains a CTX-M-15 resistance cassette and comprises 111,741 bp, with strong homology to bacteriophage-like plasmids and to the Salmonella-specific bacteriophage SSU5.
International Journal of Systematic and Evolutionary Microbiology | 2016
Swapnil Doijad; Can Imirzalioglu; Yancheng Yao; Niladri Bhusan Pati; Linda Falgenhauer; Torsten Hain; Bärbel U. Foesel; Birte Abt; Jörg Overmann; Mariam M. Mirambo; Stephen E. Mshana; Trinad Chakraborty
A total of 17 Enterobacter-like isolates were obtained from blood during a septicaemia outbreak in a neonatal unit, Tanzania, that could not be assigned based on phenotypic test to any existing Enterobacter species. Eight representative outbreak isolates were investigated in detail. Fermentation characteristics, biochemical assays and fatty acid profiles for taxonomic analysis were determined and supplemented with information derived from whole genome sequences. Phenotypic and morphological tests revealed that these isolates were Gram-stain-negative, rod-shaped, highly motile and facultatively anaerobic. The fatty acid profile was similar to those of the type strains for all recognized Enterobacter species, with quantitative differences in C17 : 0, C18 : 1ω7c and C17 : 0 cyclo fatty acids. Whole genome sequencing was used to identify taxonomically relevant characteristics, i.e. for 16S rRNA gene sequence analysis, multi-locus sequence analysis (MLSA), in silico DNA-DNA hybridization (isDDH) and average nucleotide identity (ANI). Draft genomes were approximately 4.9 Mb in size with a G+C content of 56.0 mol%. The 16S rRNA gene sequence of these eight isolates showed >97 % similarity to all Enterobacter species, while MLSA clustered them closely with the type strains of Enterobacter xiangfangensis and Enterobacter hormaechei. These eight strains showed less than 70 % isDDH identity with the type strains of Enterobacter species. In addition, less than 95 % ANI to the type strains of Enterobacter species was observed. From these results, it is concluded that these isolates possess sufficient characteristics to differentiate them from all recognized Enterobacter species, and should therefore be considered as representing a novel species. The name Enterobacter bugandensis sp. nov. is proposed with EB-247T ( = DSM 29888T = NCCB 100573T) as the type strain.
Genome Announcements | 2014
Yancheng Yao; Can Imirzalioglu; Torsten Hain; Martin Kaase; Soeren Gatermann; Martin Exner; Martin Mielke; Anja M. Hauri; Yolanta Dragneva; Rita Bill; Constanze Wendt; Angela Wirtz; Eugen Domann; Trinad Chakraborty
ABSTRACT The complete and annotated nucleotide sequence of a 54,036-bp plasmid harboring a blaKPC-2 gene that is clonally present in Citrobacter isolates from different species is presented. The plasmid belongs to incompatibility group N (IncN) and harbors the class A carbapenemase KPC-2 in a unique genetic environment.
Frontiers in Microbiology | 2017
Yancheng Yao; Fernando Lázaro-Perona; Linda Falgenhauer; Aránzazu Valverde; Can Imirzalioglu; Lucas Domínguez; Rafael Cantón; Jesús Mingorance; Trinad Chakraborty
Untreated wastewater, particularly from hospitals and other healthcare facilities, is considered to be a reservoir for multidrug-resistant bacteria. However, its role in the spread of antibiotic resistances in the human population remains poorly investigated. We used whole genome sequencing to analyze 25 KPC-2-producing Enterobacteriaceae isolates from sewage water collected during a 3-year period and three clinical Citrobacter freundii isolates from a tertiary hospital in the same collection area in Spain. We detected a common, recently described, IncP-6 plasmid carrying the gene blaKPC-2 in 21 isolates from both sources. The plasmid was present in diverse environmental bacterial species of opportunistic pathogens such as C. freundii, Enterobacter cloacae, Klebsiella oxytoca, and Raoultella ornithinolytica. The 40,186 bp IncP-6 plasmid encoded 52 coding sequences and was composed of three uniquely combined regions that were derived from other plasmids recently reported in different countries of South America. The region harboring the carbapenem resistance gene (14 kb) contained a Tn3 transposon disrupted by an ISApu-flanked element and the core sequence composed by ISKpn6/blaKPC-2/ΔblaTEM-1/ISKpn27. We document here the presence of a novel promiscuous blaKPC-2 plasmid circulating in environmental bacteria in wastewater and human populations.
Antimicrobial Agents and Chemotherapy | 2017
Linda Falgenhauer; Hiren Ghosh; Swapnil Doijad; Yancheng Yao; Boyke Bunk; Cathrin Spröer; Martin Kaase; Rolf Hilker; Jörg Overmann; Can Imirzalioglu; Trinad Chakraborty
Colistin is used as a last-resort antibiotic for treating infections caused by multidrug-resistant members of the family Enterobacteriaceae, in particular for carbapenem-resistant isolates. Resistance to colistin in Enterobacteriaceae species has been attributed to chromosomal mutations ([1][1]),
Scientific Reports | 2018
Niladri Bhusan Pati; Swapnil Doijad; Tilman Schultze; Gopala Krishna Mannala; Yancheng Yao; Sangeeta Jaiswal; Daniel Ryan; Mrutyunjay Suar; Konrad Gwozdzinski; Boyke Bunk; Mobarak Abu Mraheil; Mohamed A. Marahiel; Julian D. Hegemann; Cathrin Spröer; Alexander Goesmann; Linda Falgenhauer; Torsten Hain; Can Imirzalioglu; Stephen E. Mshana; Jörg Overmann; Trinad Chakraborty
Nosocomial pathogens can cause life-threatening infections in neonates and immunocompromised patients. E. bugandensis (EB-247) is a recently described species of Enterobacter, associated with neonatal sepsis. Here we demonstrate that the extended spectrum ß-lactam (ESBL) producing isolate EB-247 is highly virulent in both Galleria mellonella and mouse models of infection. Infection studies in a streptomycin-treated mouse model showed that EB-247 is as efficient as Salmonella Typhimurium in inducing systemic infection and release of proinflammatory cytokines. Sequencing and analysis of the complete genome and plasmid revealed that virulence properties are associated with the chromosome, while antibiotic-resistance genes are exclusively present on a 299 kb IncHI plasmid. EB-247 grew in high concentrations of human serum indicating septicemic potential. Using whole genome-based transcriptome analysis we found 7% of the genome was mobilized for growth in serum. Upregulated genes include those involved in the iron uptake and storage as well as metabolism. The lasso peptide microcin J25 (MccJ25), an inhibitor of iron-uptake and RNA polymerase activity, inhibited EB-247 growth. Our studies indicate that Enterobacter bugandensis is a highly pathogenic species of the genus Enterobacter. Further studies on the colonization and virulence potential of E. bugandensis and its association with septicemic infection is now warranted.
Lancet Infectious Diseases | 2016
Linda Falgenhauer; Said-Elias Waezsada; Yancheng Yao; Can Imirzalioglu; A. Käsbohrer; Uwe Roesler; Geovana Brenner Michael; Stefan Schwarz; Guido Werner; Lothar Kreienbrock; Trinad Chakraborty
International Journal of Antimicrobial Agents | 2016
Hiren Ghosh; Swapnil Doijad; Boyke Bunk; Linda Falgenhauer; Yancheng Yao; Cathrin Spröer; Katrin Gentil; Judith Schmiedel; Can Imirzalioglu; Jörg Overmann; Trinad Chakraborty
Genome Announcements | 2017
Yancheng Yao; Linda Falgenhauer; Volkhard A. J. Kempf; Michael Hogardt; Stephan Göttig; Can Imirzalioglu; Trinad Chakraborty