Yisong Hu

Lactic acid fermentation from food waste with indigenous microbiota: Effects of pH, temperature and high OLR

The effects of pH, temperature and high organic loading rate (OLR) on lactic acid production from food waste without extra inoculum addition were investigated in this study. Using batch experiments, the results showed that although the hydrolysis rate increased with pH adjustment, the lactic acid concentration and productivity were highest at pH 6. High temperatures were suitable for solubilization but seriously restricted the acidification processes. The highest lactic acid yield (0.46g/g-TS) and productivity (278.1mg/Lh) were obtained at 37°C and pH 6. In addition, the lactic acid concentration gradually increased with the increase in OLR, and the semi-continuous reactor could be stably operated at an OLR of 18g-TS/Ld. However, system instability, low lactic acid yield and a decrease in VS removal were noticed at high OLRs (22g-TS/Ld). The concentrations of volatile fatty acids (VFAs) in the fermentation mixture were relatively low but slightly increased with OLR, and acetate was the predominant VFA component. Using high-throughput pyrosequencing, Lactobacillus from the raw food waste was found to selectively accumulate and become dominant in the semi-continuous reactor.

Effect of pH on lactic acid production from acidogenic fermentation of food waste with different types of inocula

Effect of acidic pH (4, 5, 6 and uncontrolled) on lactic acid (LA) fermentation from food waste was investigated by batch fermentation experiments using methanogenic sludge, fresh food waste and anaerobic activated sludge as inocula. Results showed that due to the increase of hydrolysis, substrate degradation rate and enzyme activity, the optimal LA concentration and yield were obtained at pH 5, regardless of the inoculum used. The highest LA concentration (28.4g/L) and yield (0.46g/g-TS) were obtained with fresh food waste as inoculum. Moreover, after the substrate was completely utilized, the lactic acid bacteria population sharply decreased, and the LA produced was converted to volatile fatty acids (VFAs) at pH 6 within a short period. The VFA components varied with the inoculum supplied. Microbial community analysis using high-throughput pyrosequencing revealed that diversity decreased and a high abundance of Lactobacillus (83.4-98.5%) accumulated during fermentation with all inocula.

Characterization of a hybrid powdered activated carbon-dynamic membrane bioreactor (PAC-DMBR) process with high flux by gravity flow: Operational performance and sludge properties

Three PAC-DMBRs were developed for wastewater treatment under different PAC dosages with biomass concentrations averaged at 2.5, 3.5 and 5.0g/L. The DMBRs could be continuously operated at 40-100L/m2h, while higher fluxes were obtained within the PAC-DMBRs with hydraulic retention times varying in 4-10h. A dose of 1g/L PAC brought about obvious improvement in the sludge particle size distribution, settling, flocculating and dewatering properties due to the formation of biological PAC, and the sludge properties were further improved at a higher PAC dose (3g/L). The addition of PAC notably shortened the DM formation time after air backwashing and enhanced pollutant removal. Moreover, under a long solid retention time (approximately 150d), the concentrations of both soluble and bound extracellular polymeric substances (EPS) decreased substantially because of the adsorption and biodegradation effects of the biological PAC. No obvious impact on biomass activity was observed with PAC addition.

Applying fermentation liquid of food waste as carbon source to a pilot-scale anoxic/oxic-membrane bioreactor for enhancing nitrogen removal: Microbial communities and membrane fouling behaviour

Fermentation liquid of food waste (FLFW) was applied as an external carbon source in a pilot-scale anoxic/oxic-membrane bioreactor (A/O-MBR) system to enhance nitrogen removal for treating low COD/TN ratio domestic wastewater. Results showed that, with the FLFW addition, total nitrogen removal increased from lower than 20% to 44-67% during the 150days of operation. The bacterial metabolic activities were obviously enhanced, and the significant change in microbial community structure promoted pollutants removal and favored membrane fouling mitigation. By monitoring transmembrane pressure and characterizing typical membrane foulants, such as extracellular polymeric substances (EPS), dissolved organic matter (DOM), and inorganics and biopolymers in the cake layer, it was confirmed that FLFW addition did not bring about any additional accumulation of membrane foulants, acceleration of fouling rate, or obvious irreversible membrane fouling in the whole operation period. Therefore, FLFW is a promising alternative carbon source to enhance nitrogen removal for the A/O-MBR system.

Anaerobic dynamic membrane bioreactor (AnDMBR) for wastewater treatment: A review

Recently, an increasing level of attention has focused on the emerging technology of anaerobic dynamic membrane bioreactors (AnDMBRs), owing to its merits such as low membrane module cost, easy control of membrane fouling, low energy consumption and sludge production, as well as biogas production. As research on AnDMBRs is still in the nascent stage, an introduction of bioreactor configurations, dynamic membrane (DM) module, and DM layer formation and cleaning is firstly presented. The process performance of the AnDMBR for wastewater treatment is then reviewed with regard to pollutant removal, DM filterability, biogas production, and potential advantages over the conventional anaerobic membrane bioreactor (AnMBR). In addition, the important parameters affecting process performance are briefly discussed. Lastly, the challenges encountered and perspectives regarding the future development of the AnDMBR process to promote its practical applications are presented.

Dynamic membrane-assisted fermentation of food wastes for enhancing lactic acid production.

A dynamic membrane (DM) module was inserted into a fermentation reactor to separate soluble products from the fermented mixture to increase lactic acid (LA) production from food wastes under acidogenic conditions (uncontrolled pH, pH 4 and 5). With a high total suspended solid content (20-40g/L) in the fermenter, a stable DM could be maintained through regular backwashing. By effectively intercepting suspended solids and lactic acid bacteria (LAB), the fermenter was able to increase microbial activity and largely promote LA yield. Hydrolysis and acidogenesis rates increased with pH, and the highest LA yield (as high as 0.57g/g-TS) was obtained at pH 4. The microbial community analysis showed that the relative abundance of Lactobacillus increased to 96.4% at pH 4, but decreased to 43.3% at pH 5. In addition, the DM could be easily recovered by intercepting larger particles in less than 2h after each cycle of periodic backwashing.

Dynamic membrane bioreactor performance enhancement by powdered activated carbon addition: Evaluation of sludge morphological, aggregative and microbial properties

The effects of powdered activated carbon (PAC) addition on sludge morphological, aggregative and microbial properties in a dynamic membrane bioreactor (DMBR) were investigated to explore the enhancement mechanism of pollutants removal and filtration performance. Sludge properties were analyzed through various analytical measurements. The results showed that the improved sludge aggregation ability and the evolution of microbial communities affected sludge morphology in PAC-DMBR, as evidenced by the formation of large, regularly shaped and strengthened sludge flocs. The modifications of sludge characteristics promoted the formation process and filtration flux of the dynamic membrane (DM) layer. Additionally, PAC addition did not exert very significant influence on the propagation of eukaryotes (protists and metazoans) and microbial metabolic activity. High-throughput pyrosequencing results indicated that adding PAC improved the bacterial diversity in activated sludge, as PAC addition brought about additional microenvironment in the form of biological PAC (BPAC), which promoted the enrichment of Acinetobacter (13.9%), Comamonas (2.9%), Flavobacterium (0.31%) and Pseudomonas (0.62%), all contributing to sludge flocs formation and several (such as Acinetobacter) capable of biodegrading relatively complex organics. Therefore, PAC addition could favorably modify sludge properties from various aspects and thus enhance the DMBR performance.

Wastewater reclamation for various non-potable supplies and water quality control in a minimum-emission hydrologic cycle

AbstractIn this study, we implemented an isolated decentralized water system with minimized wastewater discharge and maximized water reuse in water-deficient region. Dual quality wastewater reclamation and reuse system with parallel conventional treatment unit and advanced MBR treatment unit was built in which the reclaimed water with normal quality and high quality was produced, respectively. Based on maximum collection of the recollectable discharged water, the minimum-emission scheme characterized by high cycling rate of the limited water resource was formed and consequently all the nonpotable water consumption was covered by the reclaimed water. Environmental lakes integrated with multi-functions of quantity regulation and quality stabilization of the reclaimed water were introduced in the system configuration and stepwise use of the reclaimed water was observed successfully. As a result of the system operation, main pollutants were well removed and the quality of reclaimed water can reach the demands...

Nutrients removal performance and sludge properties using anaerobic fermentation slurry from food waste as an external carbon source for wastewater treatment

Enhancement of nitrogen and phosphate removal using thermophilic fermentation slurry from food waste (FSFW) as external carbon source was investigated. Based on the batch tests, the soluble and particulate fractions of the FSFW acted as easily and slowly biodegradable carbon sources, respectively, and the fermented slurry showed the combined nutrients removal properties of soluble and solid organics. During the long-term operation of a sequencing batch reactor (SBR) with FSFW for wastewater treatment, the sludge particle size increased obviously, the bacterial metabolic capacity improved significantly, and some functional microorganisms were enriched selectively, which significantly promoted the nitrogen removal efficiency (approximately 90%) by enhancing the anoxic denitrification and simultaneous nitrification and denitrification (SND) processes. Moreover, high phosphate removal efficiency (above 98%) was achieved through the aerobic and anoxic phosphate accumulation processes. Thus, using the FSFW as supplementary carbon source is a suitable solution for both food waste disposal and wastewater treatment.

Thermodynamic prediction and experimental investigation of short-term dynamic membrane formation in dynamic membrane bioreactors: Effects of sludge properties

In dynamic membrane bioreactors (DMBRs), a dynamic membrane (DM) forms on a support material to act as the separation membrane for solids and liquids. In this study, batch filtration tests were carried out in a DMBR using nylon mesh (25 μm) as support material to filtrate sludge suspensions of variable properties from three different sources to evaluate the effects on the short-term DM formation process (within 240 min). Furthermore, the extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory was applied to analyze the sludge adhesion and cohesion behaviors on the mesh surface to predict quantitative parameters of the short-term DM formation process (including initial formation and maturation stage). The filtration results showed that the order of the initial DM formation time (permeate turbidity <1 NTU as an indicator) was as follows: sludge with poor settleability and dewaterability < normal sludge < sludge with poor flocculability. Moreover, normal sludge (regarding settleability, dewaterability, flocculability, and extracellular polymeric substance) showed a more acceptable DM formation performance (short DM formation time, low permeate turbidity, and high permeate flux) than sludge with poor settleability, dewaterability and flocculability. The influence of sludge properties on the initial DM formation time corroborates the prediction of sludge adhesion behaviors by XDLVO theory. Additionally, the XDLVO calculation results showed that acid-based interaction, energy barrier, and secondary energy minimum were important determinants of the sludge adhesion and cohesion behaviors. Therefore, short-term DM formation process may be enhanced to achieve stable long-term DMBR operation through positive modification of the sludge properties.