Yuri F. Shirai

Activation of Enteroendocrine Cells via TLRs Induces Hormone, Chemokine, and Defensin Secretion

Enteroendocrine cells are known primarily for their production of hormones that affect digestion, but they might also be implicated in sensing and neutralizing or expelling pathogens. We evaluate the expression of TLRs and the response to specific agonists in terms of cytokines, defensins, and hormones in enteroendocrine cells. The mouse enteroendocrine cell line STC-1 and C57BL/6 mice are used for in vitro and in vivo studies, respectively. The presence of TLR4, 5, and 9 is investigated by RT-PCR, Western blot, and immunofluorescence analyses. Activation of these receptors is studied evaluating keratinocyte-derived chemokine, defensins, and cholecystokinin production in response to their specific agonists. In this study, we show that the intestinal enteroendocrine cell line STC-1 expresses TLR4, 5, and 9 and releases cholecystokinin upon stimulation with the respective receptor agonists LPS, flagellin, and CpG-containing oligodeoxynucleotides. Release of keratinocyte-derived chemokine and β-defensin 2 was also observed after stimulation of STC-1 cells with the three TLR agonists, but not with fatty acids. Consistent with these in vitro data, mice showed increased serum cholecystokinin levels after oral challenge with LPS, flagellin, or CpG oligodeoxynucleotides. In addition to their response to food stimuli, enteroendocrine cells sense the presence of bacterial Ags through TLRs and are involved in neutralizing intestinal bacteria by releasing chemokines and defensins, and maybe in removing them by releasing hormones such as cholecystokinin, which induces contraction of the muscular tunica, favoring the emptying of the distal small intestine.

Toll‐like receptor agonists regulate β‐defensin 2 release in hair follicle

Background  Skin is constantly in contact with different pathogens, which are present in the environment. The hair follicle is particularly susceptible to this microbial invasion as it offers an easy way of access for microorganisms; for this reason it is equipped with defence mechanisms to avoid frequent infections.

Repeatability of mae-geri-keage in traditional karate: a three-dimensional analysis with black-belt karateka.

In karate, performance also depends on a correct body shape, and the repeatability of standardized movements conditions the effectiveness of techniques. The execution of mae-geri-keage (frontal snap kick) was studied in 13 karateka (1st–5th dan). The 3D trajectories of 13 body landmarks were analyzed by an optoelectronic instrument while each karateka performed 10 repetitions of the movement. For each karateka and repetition, the standard deviations of the spatial coordinates x, y, z for each landmark were computed. A total standard deviation of the single participant was also calculated. Two experienced karateka performed with the best repeatability (smaller standard deviation) while executing the studied kick. Generally, the best repeatability was observed in the horizontal plane. The lower variability was observed in hips and head. Ankle and knee of the dominant limb had the worse. The method could detect athletes particularly gifted for the discipline. Moreover, it could help in the identification of those parts of body that do not repeat the movement with the desired precision.

Body movements on the men’s competition mushroom: a three dimensional analysis of circular swings

Objectives: To develop a method for the three dimensional analysis of body movements and body positions during the performance of circular swings on the competition mushroom, an apparatus used by young gymnasts for pommel horse training. Methods: Five experienced male gymnasts, all of national level, performed three series of 10 circular swings on the competition mushroom. An optoelectronic instrument was used for the detection of the three dimensional movement of 13 body landmarks. From landmark trajectories, several technical measurements were obtained: diameters of ideal circles of ankles, hips, shoulders; deviation of the ankle diameters from circularity and from the horizontal plane; angle between the shoulder, hip, and ankle. The values were used for a quantitative assessment of performance of the five gymnasts. Results: During the exercise, each ankle should follow a nearly horizontal circular path (deviation from circularity ranged from 3.6% to 6%, deviation from horizontality was 9.4–19.7%), there should be an angle of about 180° at the hips (actual values 146–153°), and the shoulders should move as little as possible, and only in the lateral plane, without major anteroposterior movements (shoulder movement was 27–31% of ankle movement, hip movement was 16–20%). Conclusions: The method could help coaches and gymnasts to determine which parts of the body are not repeating a selected movement with sufficient accuracy and to quantify improvements made after a specific training programme.

Dynamic balance in elite karateka.

In karate, balance control represents a key performance determinant. With the hypothesis that high-level athletes display advanced balance abilities, the purpose of the current study was to quantitatively investigate the motor strategies adopted by elite and non-elite karateka to maintain balance control in competition. The execution of traditional karate techniques (kihon) in two groups of elite Masters (n = 6, 31 ± 19 years) and non-elite Practitioners (n = 4, 25 ± 9 years) was compared assessing body center of mass (CoM) kinematics and other relevant parameters like step width and angular joint behavior. In the considered kihon sequence, normalized average CoM height was 8% lower (p < 0.05), while CoM displacement in the horizontal direction was significantly higher in Masters than in Practitioners (2.5 vs. 1.9 m, p < 0.05), as well as CoM average velocity and rms acceleration (p < 0.05). Step width was higher in Masters in more than half of the sequence steps (p < 0.05). Results suggest that elite karateka showed a refined dynamic balance control, obtained through the increase of the base of support and different maneuvers of lower limbs. The proposed method could be used to objectively detect talented karateka, to measure proficiency level and to assess training effectiveness.

Transfer of training for two ball-throwing tasks.

The effects of training overhand ball throws to enhance underhand ball-throwing accuracy were studied with 921 Italian high school pupils (575 girls, M age = 16.5 yr., SD =1.3; 346 boys, M = 16.5 yr., SD = 1.2) ages 14 to 18 years. A standardized test (10 underhand tennis-ball throws into the floor area delimited by an 80-cm radius wooden hoop lying on the floor 9 m from the standing pupil) was performed and scored. An Experimental group (435 girls, M age = 16.5 yr., SD = 1.3; 204 boys, M age = 16.5 yr., SD =1.2) was randomly selected to participate in 10 weekly training periods. The set included 10 overhand throws of a rubber bouncing ball (14.5 cm diameter, weight 240 g) into a basketball hoop from 5 standardized positions (in front of the basketball hoop; at 45°, 90° on the right and on the left of the basketball hoop) at a distance of 4.40 m. The underhand test was repeated for both groups. On the average, boys obtained higher test scores than girls of the same age. Older adolescents had higher mean scores than younger adolescents. After 10 weeks, boys and girls of all ages improved. Mean differences between sessions were significant for the Experimental group (Student t test, p <.01). In Session 2, consistent differences between Experimental and Control groups were also found (Student t test, p < .05).

Cinemática de variáveis de técnica-chave nos flic flacs de ginastas de elite

INTRODUCTION: Gymnastics is the most ancient and spectacular closed skills sport. Nonetheless, technical parameters of performance are often taught only by experienced trainers. Thus, there is a lack of objective data about gymnastics performance (kinematics references). OBJECTIVE: In the current study, we tried to quantify linear and hyperextension back movements during performance of backward handsprings. METHODS: A noninvasive detection of backward handsprings was made using a 3D optoelectronic instrument. Thirteen spherical retro-reflective markers (1-cm diameter) were positioned on the body of 9 experienced gymnasts: right and left lateral malleolus, fibular head, greater trochanter, acromion, olecranon, styloid process of the ulna; vertex. In the same session and after a warm-up period, each participant performed 15 repetitions of backward handsprings. Ten repetitions were analyzed, and the 3D tracks of the 13 landmarks measured. RESULTS: On average, men performed longer backward handsprings than women (men, 122% of height; women, 98%); women attained larger vertical height (women, 62% of height; men, 58%). Lower limb arrangement was homogenous among the gymnasts: posterior knee angles ranged between 80° and 118°. No lower limb abduction was observed: knee width was 7 cm smaller than intertrochanter width; ankle width was 8 cm smaller than knee width. At take-off, the trunk-thigh angle presented excellent body alignment, with values very close to 180°. Women performed the handstand phase with closer wrists than men (men, 134% of shoulder width; women, 121%). CONCLUSION: The results may offer data to improve understanding, defining gold-standard execution taken from high level gymnasts with few injuries.