Yuyun Yueniwati

Effect of genistein on proinflammatory cytokines and estrogen receptor–β in mice model of endometriosis

Abstract Objective To investigate the effect of genistein on proinflammatory cytokines, NF- κ B activation, and estrogen receptor-β expression in a mice model of endometriosis. Methods Forty female mice ( Mus musculus ) were divided into eight groups ( n =5 each), including the control (untreated) group, endometriosis group, and the endometriosis groups were given various doses of genistein (at doses of 50; 100; 200; 300; 400; 500 mg/day). Analysis of TNF-α, IL-1β, IL-6, and IL-8 level were done by ELISA technically. Analysis of estrogen receptor-β and NF-κB were done by immunohistochemistry. Results The level of TNF-α, IL-1β, IL-6, and IL-8 were significantly higher in the EM group compared to the untreated control group ( P P P >0.05). These increased levels of IL-1β, IL-6, adn IL-8 in the EM group were significantly reduced by all doses of genistein. There were significantly ( P P P >0.05). All doses genistein significantly prevented EM-induced increase in NF-κB activation ( P Conclusions In conclusion, genistein prohibits the increase in proinflammatory cytokines, NF-κB, and estrogen receptor-β expression in a mice model of endometriosis.

Thicker carotid intima-media thickness and increased plasma VEGF levels suffered by post-acute thrombotic stroke patients

Background and objectives Atherosclerosis causes reduction of the oxygen supply to structures in the far arterial wall, provoking the release of factors that drive angiogenesis of vasa vasorum, including VEGF. Other studies have revealed the inflammatory response in atherosclerosis and the role of platelet factor 4 (PF4) as an anti-angiogenic chemokine through the inhibition of VEGF. This cross-sectional study aims at measuring the effect of atherosclerosis assessed through carotid intima-media thickness (CIMT) against plasma VEGF levels in patients with post-acute thrombotic stroke. Materials and methods CIMT was assessed sonographically using GE Logiq S6 with 13 MHz frequency linear probe. VEGF-A plasma levels were measured using enzyme-linked immunosorbent assay (ELISA) method. Differences among variables were compared statistically. The data were analyzed using Pearson correlation. Results A total of 25 patients with post-acute thrombotic stroke were identified in days 7 to 90. CIMT thickening was indicated in 88% of patients (1.202 ± 0.312 mm), while an increase in plasma VEGF was identified in all patients (178.28 ± 93.96 ng/mL). There was no significant correlation between CIMT and plasma VEGF levels in patients with post-acute thrombotic stroke (p=0.741). A significant correlation was recognized between CIMT and total cholesterol (p=0.029) and low-density lipoprotein (p=0.018). Conclusion There were no significant correlations between CIMT and plasma VEGF levels in patients with post-acute thrombotic stroke. However, plasma VEGF increased in patients with thrombotic stroke. CIMT measurement is a promising noninvasive modality to assess the vascular condition of patients with stroke and diabetes, while plasma VEGF cannot specifically assess vascular condition as it can be triggered by ischemic conditions in tissues of the whole body.

The effects of genistein as antiinflammatory and antiangiogenesis in primary endometriosis cell culture

Objective To investigate whether genistein is anti-inflammation and anti-angiogenesis in endometriosis cells.

Combination of vitamin C and E modulated monosodium glutamate- induced endometrial toxicily in female Wistar rats

Objective To investigate whether combination of vitamin C and E able to inhibit decreasing angiogenesis, endometrial thickness, and α-estrogen receptor level in female rats receiving orally MSG-treatment.

The effects of Vigna unguiculata on cardiac oxidative stress and aorta estrogen receptor-β expression of ovariectomized rats

Abstract Objective To investigate whether Vigna unguiculata ( V. Unguiculata , VU) able to reduced oxidative stress in cardiac and modulate the aorta estrogen receptor-β of ovariectomized rats. Methods Thirty female Wistar rats were divided into 5 groups ( n =6); control (sham) group; ovariectomy group (OVX); OVX + VU 1 (0.5 mg/kg); OVX + VU 2 (2.5 mg/kg); and OVX + VU 3 (5 mg/kg). The administration VU was started 28 days after surgery following 30 days later. Cardiac malondialdehyde (MDA) and superoxide dismutase (SOD) levels were measured colorimetrically. Estrogen receptor-β in the aorta was analyzed immunohistochemically. Results Level of MDA was significantly higher in the OVX group compared to the control group ( P P 2 . Administration of VU significantly increases the level of SOD compared with OVX group ( P P >0.05). The level of estrogen receptor-β was significantly decreased in the OVX group compared to the control group ( P 3 could significantly increase the level of estrogen receptor-β compared to OVX group ( P P >0.05). Conclusions V. unguiculata is an alternative therapy in decreasing cardiac oxidative stress in ovariectomized rats. Besides, high dose of V. unguiculata also able to increase aorta estrogen receptor-β expression in ovariectomized rats.

The role of Brain Magnetic Resonance Imaging (MRI) as an early detector of cognitive impairment

Background: Along with the increase of the health and prosperity level will affect the life expectancy in Indonesia, there has also been an increase in degenerative disease cases. One of the problems arises is cognitive impairment. The mild version of this impairment is often associated with the increase risk that will eventually lead to dementia. Therefore, early detection of this impairment is necessary. Objective: This study is aimed at proving the correlation between Fazekas scale on brain MRI and MoCA-Ina score in defining the degree of cognitive impairment. Methods: This study employed observational analytic design and cross sectional study for its data collection method. The Fazekas scale on brain MRI of 32 patients was read by 3 radiologist, while the MoCA-Ina scoring was done by a competent neurologist. Both tests were done double blindly. Later on, the correlation between Fazekas scale and MoCA-Ina score would be assessed using Spearman Correlation. Results: Statistical calculation conducted using Spearman Correlation reveals that the coefficient is -0.519 with significant score (P) 0.002, which is smaller than α: 0.05. Therefore, it can be concluded that there is a strong negative correlation between Fazekas scale and MoCA-Ina score. Conclusion: Fazekas scale evaluation on brain MRI is necessary to be performed as it helps predicting the decline of ones cognitive function, so that an early therapy can be acted upon to prevent dementia in the future.

The challenges in differentiating tuberculous from pyogenic spondylitis using magnetic resonance imaging

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Diagnostic Test Value of Assessment Adenoid Enlargement with and Without Airway Obstruction Using Lateral Soft Tissues X-ray Compared to Nasoendoscopy

Adenoid hypertrophy is one of the most important causes of airway obstruction. Skull lateral soft tissues X ray are reliable diagnostic tools for detection adenoid enlargement with or without airway obstruction. Compared to nasoendoscopy that invasive and not available in all hospital. The aim of this study is to evaluate the diagnostic value of Skull lateral soft tissues X ray for assessment adenoid enlargement using Kurien, Cohen and Konak, Fujioka and McNamara methods compared with nasoendoscopy. Analytic observational with cross sectional study. 24 patients suspected adenoid enlargement was performed Skull lateral soft tissues X ray and measured the enlargement with Kurien, Cohen and Konak, Fujioka and McNamara methods. After performed X-ray these patients was been nasoendoscopy. Diagnostic value of Skull lateral soft tissues X ray Kurien methods show sensitivity was 73.6%, specificity 100%, positive predictive value 100%, negative predictive value 50% and accuracy 79.1%. Diagnostic value of Skull lateral soft tissues X ray Cohen and Konak methods show sensitivity was 94.7%, specificity 100%, positive predictive value 100%, negative predictive value 83.3% and accuracy 95.8%. Diagnostic value of Skull lateral soft tissues X ray Fujioka methods show sensitivity was 10.5%, specificity 100%, positive predictive value 100%, negative predictive value 22.7% and accuracy 29.1%. Diagnostic value of Skull lateral soft tissues X ray McNamara methods show sensitivity was 68.4%, specificity 100%, positive predictive value 100%, negative predictive value 45.4% and accuracy 75%. Adenoid enlargement can be measure with Skull lateral soft tissues X ray using Cohen and Konak method that has the highest sensitivity, specificity, and accuration compare another methods and using McNamara methods can predict the airway obstruction.

Bowel preparation in intravenous urography doesn’t have an impact on the quality of urinary tract image

Purpose: The procedure of bowel preparation before intravenous urography (IVU) is still a controversial debate and it varies in each health center. Preparation is believed to reduce residual feces and intestinal gas thus improving the quality of visualization of the urinary tract. On the other hand, many radiology and urology studies did not mention the need for preparation before IVU procedure. Preparation before IVU, especially giving laxative agent, gives many adverse effects to the patient. The purpose of this study was to know the difference in quality visualization of the urinary tract in IVU patients with and without preparation in Saiful Anwar hospital Malang. Materials and Methods: A total of 20 patients participated in this study and they were divided into two groups in which each group consists of 10 patients with and without preparation before IVU. Abdominal x-ray and IVU were performed on all patients. Urinary tract visualization quality assessment was done through two methods of assessing the quality of the image by the European Commission Guidelines and of assessing the residue of feces/ intestinal gas based on Dadkhah’s studies. Results: Results showed the total score of image quality and the residue of feces/intestinal gas was not significantly different in the abdominal plain photo of IVU patients with and without preparation (Mann-Whitney test (p=1.000) and Independent T-test (p=0.5111)). Conclusions: In conclusion, there was no difference in the quality of visualization of the urinary tract in IVU patients with and without preparation in Saiful Anwar hospital Malang. Bangladesh Journal of Medical Science Vol.16(1) 2017 p.107-114

Genetic variations in the osteopontin promoters T-443C and G-156GG increase carotid intima-media thickness

Carotid intima–media thickness (CIMT) is a clear predictor of atherosclerosis. The increase of CIMT is affected by mutations in the osteopontin (OPN) promoters. The purpose of this study was to examine genetic variations in OPN promoters T-443C and G-156GG, identified in Javanese children with ischemic stroke parents, and to investigate their relationship with the increase of CIMT. A case–control analytic study was performed on 20 case and 12 control samples. Case samples were Javanese children aged between 10 to 21 years with ischemic stroke parents. Control samples were children with healthy parents. Mutations of T-443C and G-156GG were determined by employing polymerase chain reaction. Results of sequencing were analyzed using CLC Main Workbench 6.0. CIMT was defined using ultrasound. Genetic variations of T-443C were identified in six samples. Likewise, genetic variations of G-156GG were identified in six samples. Genetic variations in the OPN promoters T-443C and G-156GG were not potential risk factors in an increase of CIMT (P=0.654 and P=0.654). This study proves that genetic variations could be identified at the points of T-443C and G-156GG in children with ischemic stroke parents. Although statistically insignificant, the tendency to increase CIMT occurs in children with genetic variations. Children with ischemic stroke parents have thicker CIMT than children of healthy parents.