Noorhamdani

Intervening with healthcare workers' hand hygiene compliance, knowledge, and perception in a limited-resource hospital in Indonesia: A randomized controlled trial study

BackgroundHand hygiene is recognized as an important measure to prevent healthcare-associated infections. Hand hygiene adherence among healthcare workers is associated with their knowledge and perception. This study aimed to evaluate the effect of three different educational programs on improving hand hygiene compliance, knowledge, and perception among healthcare workers in a tertiary care hospital in Indonesia.MethodsThe study was performed from May to October 2014 and divided into a pre-intervention, intervention, and post-intervention phase. This cluster randomized controlled trial allocated the implementation of three interventions to the departments, including role model training-pediatrics, active presentation-surgery, a combination of role model training and active presentation-internal medicine, and a control group-obstetrics-gynecology. Both direct observation and knowledge-perception survey of hand hygiene were performed using WHO tools.ResultsHand hygiene compliance was observed during 2,766 hand hygiene opportunities, and knowledge-perception was assessed among 196 participants in the pre-intervention and 88 in the post-intervention period. After intervention, the hand hygiene compliance rate improved significantly in pediatrics (24.1% to 43.7%; P < 0.001), internal medicine (5.2% to 18.5%; P < 0.001), and obstetrics-gynecology (10.1% to 20.5%; P < 0.001). The nurses’ incorrect use of hand rub while wearing gloves increased as well (P < 0.001). The average knowledge score improved from 5.6 (SD = 2.1) to 6.2 (SD = 1.9) (P < 0.05). In the perception survey, “strong smell of hand alcohol” as a reason for non-compliance increased significantly in the departments with intervention (10.1% to 22.9%; P = 0.021).ConclusionThe educational programs improved the hand hygiene compliance and knowledge among healthcare workers in two out of three intervention departments in a limited-resource hospital in Indonesia. Role model training had the most impact in this setting. However, adjustments to the strategy are necessary to further improve hand hygiene.

The effects of genistein as antiinflammatory and antiangiogenesis in primary endometriosis cell culture

Objective To investigate whether genistein is anti-inflammation and anti-angiogenesis in endometriosis cells.

The effects of Vigna unguiculata on cardiac oxidative stress and aorta estrogen receptor-β expression of ovariectomized rats

Abstract Objective To investigate whether Vigna unguiculata ( V. Unguiculata , VU) able to reduced oxidative stress in cardiac and modulate the aorta estrogen receptor-β of ovariectomized rats. Methods Thirty female Wistar rats were divided into 5 groups ( n =6); control (sham) group; ovariectomy group (OVX); OVX + VU 1 (0.5 mg/kg); OVX + VU 2 (2.5 mg/kg); and OVX + VU 3 (5 mg/kg). The administration VU was started 28 days after surgery following 30 days later. Cardiac malondialdehyde (MDA) and superoxide dismutase (SOD) levels were measured colorimetrically. Estrogen receptor-β in the aorta was analyzed immunohistochemically. Results Level of MDA was significantly higher in the OVX group compared to the control group ( P P 2 . Administration of VU significantly increases the level of SOD compared with OVX group ( P P >0.05). The level of estrogen receptor-β was significantly decreased in the OVX group compared to the control group ( P 3 could significantly increase the level of estrogen receptor-β compared to OVX group ( P P >0.05). Conclusions V. unguiculata is an alternative therapy in decreasing cardiac oxidative stress in ovariectomized rats. Besides, high dose of V. unguiculata also able to increase aorta estrogen receptor-β expression in ovariectomized rats.

Effect of human ZP3 monoclonal antibody on expression of GDF-9 and number of theca cells in ovary of mice ( Mus musculus )

Objectives This study investigated the effects of a human ZP3 monoclonal antibody (mAb hZP3) on the expression of growth differentiation factor-9 (GDF-9) and number of theca cells in the ovaries of mice (Mus musculus). Methods Our study employed a true experiment posttest-only control group design of 48 mice that were divided into the control and three mAb hZP3-treatment groups (20, 40, and 60 μg). Mice in each group were terminated on days 10, 15, and 20. GDF-9 expression was measured by immunohistochemistry and the number of theca cells was counted. Results Analysis of the effects of mAb hZP3 (at 20–60 μg) on the expression of GDF-9 and amount of theca cells did not show significant differences. Similar findings were observed throughout the study period (at 10–20 days). Therefore, mAb hZP3 had no effect on GDF-9 expression and theca cells. Conclusion This study showed that mAb hZP3 can be considered to be an effective and safe immunocontraception.