Z. Xiong

An

The application of cold plasma in sterilization of a root canal of a tooth has recently attracted great attention. In this paper, a reliable and user-friendly plasma-jet device, which can generate plasma inside the root canal, is reported. The plasma can be touched by bare hands and can be directed manually by a user to place it into root canal for disinfection without causing any painful sensation. When He/O2(20%) is used as working gas, the rotational and vibrational temperatures of the plasma are about 300 K and 2700 K, respectively. The peak discharge current is about 10 mA. Preliminary inactivation experiment results show that it can efficiently kill enterococcus faecalis , one of the main types of bacterium causing failure of root-canal treatment in several minutes.

RC

Rather than using noble gas, room air is used as the working gas for an atmospheric pressure room-temperature plasma. The plasma is driven by submicrosecond pulsed directed current voltages. Several current spikes appear periodically for each voltage pulse. The first current spike has a peak value of more than 1.5 A with a pulse width of about 10 ns. Emission spectra show that besides excited OH, O, N2(C–B), and N2+(B–X) emission, excited NO, N2(B–A), H, and even N emission are also observed in the plasma, which indicates that the plasma may be more reactive than that generated by other plasma jet devices. Utilizing the room-temperature plasma, preliminary inactivation experiments show that Enterococcus faecalis can be killed with a treatment time of only several seconds.

Plasma Device for Sterilization of Root Canal of Teeth

The propagation behavior of cold atmospheric pressure plasma jets has recently attracted lots of attention. In this paper, a cold He plasma jet generated by a single plasma electrode jet device is studied. The spatial-temporal resolved optical emission spectroscopy measurements are presented. It is found that the emission intensity of the He 706.5 nm line of the plasma behaves similarly both inside the syringe and in the surrounding air (plasma plume). It decreases monotonously, which is different from the emission lines, such as N2 337.1 nm line, N2+ 391.4 nm line, and O 777.3 nm line. For the discharge inside the syringe, the emission intensity of the He 706.5 nm line decays more rapidly than that of the other three spectral lines mentioned above. The N2 337.1 nm line behaves a similar time evolution with the discharge current. For the N2+ 391.4 nm line and the atomic O 777.3 nm line, both of them decay slower than that of the He 706.5 nm and the N2 337.1 nm. When the plasma plume propagates further awa...

A simple atmospheric pressure room-temperature air plasma needle device for biomedical applications

It is well known that plasma can deactivate various types of microorganisms. However, one fundamental key question has never been addressed, namely, how deep can plasma penetrate into multilayer biofilms. In this letter, Porphyromonas gingivalis (PG) biofilms (10 days growth, which has about 30 layers of PG cells with a thickness of about 15 μm) are treated with a cold plasma plume. It is found that the plasma can penetrate the biofilms and effectively deactivate all the bacteria in the 15 μm thick biofilms. Moreover, it was found that most of the dead cells’ structures in the biofilms are not damaged. From the optical emission spectra of the plasma, it can be concluded that it is O and OH, rather than O2−, N2+, or UV emission that play the major role in the deactivation processes.

Temporal and spatial resolved optical emission behaviors of a cold atmospheric pressure plasma jet

It has recently been demonstrated that pulsed direct-current (dc) voltages show better performance in generating diffuse plasmas under various conditions. However, it still remains unclear whether the pulsewidth or the rising and falling times of the voltage pulse play the essential role in the improvement of the performance of the dielectric barrier discharges (DBDs). In this paper, we focus on the effect of pulsewidth. Pulsed dc voltages with pulsewidth varying from 0.2 mus to about 1 ms are used to drive the DBDs. High-speed photographs show that diffuse Ar plasmas can be generated by pulsed dc voltages with pulsewidths covering the entire investigated range. It is found that the pulsewidths of the applied voltages affect the discharge current durations significantly when the pulsewidth is shorter than 600 ns or the break between the two consecutive pulses is shorter than 600 ns.

How deep can plasma penetrate into a biofilm

The fundamental of the generation and propagation of the atmospheric pressure nonequilibrium plasma jets has recently attracted significant interests. In this paper, investigations on the effects of the parameters of the pulsed dc voltages on the optical emission intensity of the plasma jet and the bullet propagation behavior are carried out based on the temporal-spatial resolved optical emission spectroscopy measurements and the high-speed photography. It is found that, with the increase in the applied voltage, the bullet propagates out from the nozzle earlier and accelerates to higher peak-velocities. The increase in the pulse frequency exerts no significant influences on the optical emission of the plasma jet and the bullet propagation velocity. But it can induce the bullet propagates out from the nozzle earlier. Besides, it is interesting to notice that, with the increase in the pulse width in the beginning, the bullet propagates out from the nozzle with longer delay time. However, when the pulse widt...

Effect of Nano- to Millisecond Pulse on Dielectric Barrier Discharges

To better understand the variation in the “plasma bullet” velocity, the dynamics of an atmospheric pressure plasma plume driven by positive and negative pulses are investigated in detail. It is found that, before the plasma exits the nozzle, the plasma propagates at a speed of about 30 km/s for both positive and negative pulses. As soon as the plasma exits the nozzle, the plasma propagation speed increases dramatically for both cases. The peak velocity for the case of the positive pulse is much higher than that of the negative pulse, it is approximately 150 km/s and 70 km/s, respectively. According to the optical emission spectra, the acceleration behavior of the plasma bullet when it exits the nozzle is due to the increase in the N2+ concentration.

Experimental investigations on the propagation of the plasma jet in the open air

Using the plasma generated inside the root canal rather than the afterglow of the plasma will significantly improve the inactivation efficiency. In this paper, images of three kinds of atmospheric-pressure low-temperature plasma devices driven by different power supplies in the tooth root canal are reported. The images of an R-needle device driven by a dc power supply inside the root canal using different gases (He, Ar, and surrounding air) are also presented.

On the velocity variation in atmospheric pressure plasma plumes driven by positive and negative pulses

The discharge mode transition from uniform plasma across the gas gap to the α mode happens at the rising phase of the pulsed radio frequency capacitively coupled plasma (PRF CCP). This transition is attributed to the fast increasing stochastic heating at the edge of sheath. In the second stage with the stable current and voltage amplitude, the consistency between experimental and numerical spatial-temporal 777 nm emission profile suggests that He* and He2* dominate the production of O(5p1) through dissociation and excitation of O2. Finally, the sterilization efficiency of PRF CCP is found to be higher than that of plasma jet.

Plasmas in Tooth Root Canal

It is found that proliferation of HepG2 cells is promoted in the N-acetylcysteine (NAC) and plasma cotreatment group while the group without NAC undergoes cell apoptosis or cell death after plasma treatment. Further investigation found that NAC and plasma cotreatment accelerates the G1 to S phase transition of HepG2 cells and cyclinD1, which responds for the proliferation, is shown to be significantly up-regulated in the NAC and plasma cotreatment group. This finding provides a rationale for developing a proliferation-promotion approach and sheds new sights on the potential application of cold plasmas such as large-scale culture of stem cells in vitro for stem cell transplantation, enhancing transplanted tissue incorporation.