Yuriy A. Ushenko

Polarization-correlation mapping of biological tissue coherent images

We investigate the statistical polarization parameters of biological tissue histological section images with different morphological structure. First we outline the results of polarization coordinate mapping and analysis of the statistics of the first to fourth orders of biological tissue image polarization azimuth and ellipticities. Second, we study the statistics of the first to fourth orders of coordinate distributions of the complex degree of mutual polarization (CDMP) of biological tissue images. Finally, we consider the diagnostic possibilities of investigating 2-D distributions of CDMP of images that correspond to physiologically normal and degeneratively and/or dystrophycally changed biological tissues that are being analyzed.

Mueller-matrix of laser-induced autofluorescence of polycrystalline films of dried peritoneal fluid in diagnostics of endometriosis.

Abstract. This research presents investigation results of the diagnostic efficiency of an azimuthally stable Mueller-matrix method of analysis of laser autofluorescence of polycrystalline films of dried uterine cavity peritoneal fluid. A model of the generalized optical anisotropy of films of dried peritoneal fluid is proposed in order to define the processes of laser autofluorescence. The influence of complex mechanisms of both phase (linear and circular birefringence) and amplitude (linear and circular dichroism) anisotropies is taken into consideration. The interconnections between the azimuthally stable Mueller-matrix elements characterizing laser autofluorescence and different mechanisms of optical anisotropy are determined. The statistical analysis of coordinate distributions of such Mueller-matrix rotation invariants is proposed. Thereupon the quantitative criteria (statistic moments of the first to the fourth order) of differentiation of polycrystalline films of dried peritoneal fluid, group 1 (healthy donors) and group 2 (uterus endometriosis patients), are determined.

Concerted spatial-frequency and polarization-phase filtering of laser images of polycrystalline networks of blood plasma smears.

Abstract. The complex technique of concerted polarization-phase and spatial-frequency filtering of blood plasma laser images is suggested. The possibility of obtaining the coordinate distributions of phases of linearly and circularly birefringent protein networks of blood plasma separately is presented. The statistical (moments of the first to fourth orders) and scale self-similar (logarithmic dependences of power spectra) structure of phase maps of different types of birefringence of blood plasma of two groups of patients—healthy people (donors) and those suffering from rectal cancer—is investigated. The diagnostically sensitive parameters of a pathological change of the birefringence of blood plasma polycrystalline networks are determined. The effectiveness of this technique for detecting change in birefringence in the smears of other biological fluids in diagnosing the appearance of cholelithiasis (bile), operative differentiation of the acute and gangrenous appendicitis (exudate), and differentiation of inflammatory diseases of joints (synovial fluid) is shown.

The Interconnection between the Coordinate Distribution of Mueller-Matrixes Images Characteristic Values of Biological Liquid Crystals Net and the Pathological Changes of Human Tissues

We have theoretically grounded conceptions of characteristic points observed in coordinate distributions of Mueller matrix elements for a network of human tissue biological crystals. The interrelation between polarization singularities of laser images inherent to these biological crystals and characteristic values of above matrix elements is found. We have determined the criteria for statistical diagnostics of pathological changes in the birefringent structure of biological crystal network by using myometrium tissue as an example.

The Degree of Mutual Anisotropy of Biological Liquid Crystals Net during the Diagnostics of Human Tissues Birefringence

To characterize the degree of consistency of parameters of the optically uniaxial birefringent liquid crystals (protein fibrils) nets of biological tissues a new parameter-complex degree of mutual anisotropy is suggested. The technique of polarization measuring the coordinate distributions of the complex degree of mutual anisotropy of biological tissues is developed. It is shown that statistic approach to the analysis of complex degree of mutual anisotropy distributions of biological tissues of various morphological and physiological states and optical thicknesses appears to be more sensitive and efficient in differentiation of physiological state in comparison with investigations of complex degree of mutual polarization of the corresponding laser images.

Stokes polarimetry of biotissues

Recently the photometric and spectrophotometric methods of biotissue diagnostics, based on searching interrelation of scalar characteristics of optical radiation field with their structural parameters. The complex of investigation of transformation processes of linear-polarized radiation in biotissues has demonstrated the new possibilities of diagnostics of their pathological biotissues. The report presents deals with researching possibilities of laser polarized diagnostics of arising and proceeding of pathological changes of biotissues morphological structure.

Role of caustics in the formation of networks of amplitude zeros for partially developed speckle fields

The topology of a partially developed speckle field was studied by use of interference techniques through computer simulation. Amplitude and phase structures in the vicinity of caustics for a coherent radiation field scattered at a surface with large inhomogeneities were investigated. It was confirmed that the caustics are indispensible components of the procedure for the formation of networks of amplitude zeros for a coherent field scattered by a rough surface with large inhomogeneities. It is shown that the formation of interference forklets in the field gives evidence of changes in the fields topology, as these forklets are a diagnostic sign of transition from a caustic to a three-dimensional pattern of a diffraction catastrophe.

Polarized microstructure of laser radiation scattered by optically active biotissues

The present paper deals with the research of laser radiation polarized structure, transformed by biotissue crystalline phase. It is urgent in creating optical methods of diagnostics of biotissue orientation and mineralized structure, and in modeling biocomposite materials as well.

Metrology of biological tissue coherent images by means of estimation of complex degree of mutual polarization

The paper presents the studies on correlation structure of biological tissues polarization images. The technique of polarization measurement of coordinate distribution of degree of mutual polarization has been proposed. The topological (singular) description of polarization inhomogeneous biological tissue images has been analyzed. It has been shown that average statistical size of S-contour agrees with half-width of autocorrelation function of degree of mutual polarization coordinate distribution.

Coherent introscopy of phase-inhomogeneous surfaces and layers

The present paper deals with the research of laser radiation polarized structure, transformed by biotissue crystalline phase. It is urgent in creating optical methods of diagnostics of biotissue orientation and mineralized structure, and in modeling biocomposite materials as well.