V. A. Ushenko

Mueller-matrix diagnostics of optical properties of polycrystalline networks of human blood plasma

The work is oriented toward a study of the possibilities of Mueller-matrix diagnostics of optical anisotropy of birefringent polycrystalline networks of human plasma amino acids. The interrelations between the statistical moments from the first to the fourth orders and fractal dimensionalities, which characterize the coordinate distributions of elements of the Mueller matrix for the blood plasma and the physiological state of the human organism, are determined.

Wavelet-analysis of polarization maps of human blood plasma

Possibilities of local wavelet-analysis of coordinate distributions of polarization azimuths and ellipticity of laser images of blood plasma in healthy and cancer patients are considered. The set of statistical, correlation, and fractal parameters of distributions of wavelet-coefficients characterizing different scales of geometric dimensions of amino acid polycrystalline networks is determined. The criteria for the differentiation of transformation processes of birefringence of optically anisotropic structures of blood plasma at different scales are established.

Azimuth-invariant mueller-matrix differentiation of the optical anisotropy of biological tissues

A Mueller-matrix model is proposed for analysis of the optical anisotropy of protein networks of optically thin nondepolarizing layers of biological tissues with allowance for birefringence and dichroism. The model is used to construct algorithms for reconstruction of coordinate distributions of phase shifts and coefficient of linear dichroism. Objective criteria for differentiation of benign and malignant tissues of female genitals are formulated in the framework of the statistical analysis of such distributions. Approaches of evidence-based medicine are used to determine the working characteristics (sensitivity, specificity, and accuracy) of the Mueller-matrix method for the reconstruction of the parameters of optical anisotropy and show its efficiency in the differentiation of benign and malignant tumors.

Mueller-matrices polarization selection of two-dimensional linear and circular birefringence images

The optical model of polycrystalline networks of myometium is suggested. The results of investigating the interrelation between the values correlation (correlation area, asymmetry coefficient and autocorrelation function excess) and fractal (dispersion of logarithmic dependencies of power spectra) parameters are presented. They characterize the distributions Mueller marixes elements of myometrium histological sections. The criteria of differentiation of death coming reasons are determined.

Polarization-dependent laser autofluorescence of the polycrystalline networks of blood plasma films in the task of liver pathology differentiation.

Current research presents the results of the investigation of diagnostic efficiency of laser polarization autofluorescence for the set of endogenous fluorophores of blood plasma polycrystalline films in two spectral regions (0.5-0.53 μm and 0.63-0.67 μm) under the excitation of laser radiation with a wavelength of 0.405 μm. A model of generalized optical anisotropy of protein networks of blood plasma polycrystalline films is proposed for the purpose of defining laser autofluorescence processes. Both phase (linear birefringence and optical activity) and amplitude (linear and circular dichroisms) anisotropies have been considered. Interconnections between the optimal condition of probing beam polarization state and the efficiency of induction laser autofluorescence have been found. Statistical analysis of coordinate distributions of laser polarization autofluorescence intensities is suggested by means of determination of the quantitative criteria (statistical moments of the 1st-4th orders). The efficiency of laser polarization autofluorescence of polycrystalline networks in the task of differentiation of nonalcoholic fatty liver disease and chronic hepatitis of human liver has been analyzed.

Mueller-matrix of laser-induced autofluorescence of polycrystalline films of dried peritoneal fluid in diagnostics of endometriosis.

Abstract. This research presents investigation results of the diagnostic efficiency of an azimuthally stable Mueller-matrix method of analysis of laser autofluorescence of polycrystalline films of dried uterine cavity peritoneal fluid. A model of the generalized optical anisotropy of films of dried peritoneal fluid is proposed in order to define the processes of laser autofluorescence. The influence of complex mechanisms of both phase (linear and circular birefringence) and amplitude (linear and circular dichroism) anisotropies is taken into consideration. The interconnections between the azimuthally stable Mueller-matrix elements characterizing laser autofluorescence and different mechanisms of optical anisotropy are determined. The statistical analysis of coordinate distributions of such Mueller-matrix rotation invariants is proposed. Thereupon the quantitative criteria (statistic moments of the first to the fourth order) of differentiation of polycrystalline films of dried peritoneal fluid, group 1 (healthy donors) and group 2 (uterus endometriosis patients), are determined.

Jones-matrix mapping of complex degree of mutual anisotropy of birefringent protein networks during the differentiation of myocardium necrotic changes

The azimuthally stable method of Jones-matrix mapping of histological sections of myocardium tissue biopsy using spatial-frequency selection of the mechanisms of linear and circular birefringence is proposed. Correlation parameter-the complex degree of mutual anisotropy-and its diagnostic application are analytically substantiated. The method of measuring the complex degree of mutual-anisotropy coordinate distributions using spatial filtration of their high- and low-frequency components is developed. The interconnections between such distributions and parameters of linear and circular birefringence of myocardium tissue histological sections are defined. The comparative investigations of coordinate distributions of the complex degree of mutual anisotropy formed by fibrillar networks of myocardium tissues of different necrotic states (dead due to coronary heart disease and acute coronary insufficiency) are performed. The values and ranges of change of the statistical (moments of the first-fourth order) parameters of coordinate distributions of the complex degree of mutual anisotropy are defined. The objective criteria of cause of death differentiation are determined.

Azimuthally invariant Mueller-matrix mapping of biological tissue in differential diagnosis of mechanisms protein molecules networks an sotropy

The optical model of polycrystalline networks of blood plasma proteins is suggested. The results of investigating the interrelation between the values of correlation (correlation area, asymmetry coefficient and autocorrelation function excess) and fractal (dispersion of logarithmic dependencies of power spectra) parameters are presented. They characterize the coordinate distributions of Mueller-matrixes elements of blood plasma smears and pathological state of the organism. The diagnostic criteria of breast cancer nascency are determined.

Statistical analysis of polarization-inhomogeneous Fourier spectra of laser radiation scattered by human skin in the tasks of differentiation of benign and malignant formations

Abstract. The optical model of formation of polarization structure of laser radiation scattered by polycrystalline networks of human skin in Fourier plane was elaborated. The results of investigation of the values of statistical (statistical moments of the 1st to 4th order) parameters of polarization-inhomogeneous images of skin surface in Fourier plane were presented. The diagnostic criteria of pathological process in human skin and its severity degree differentiation were determined.

Birefringence images of polycrystalline films of human urine in early diagnostics of kidney pathology

We propose an optical model of the Mueller-matrix description of mechanisms of optical anisotropy of polycrystalline films of urine, namely, optical activity and birefringence. The algorithm of reconstruction of distributions of parameters-optical rotation angles and phase shifts of the indicated anisotropy types-are elaborated upon. The objective criteria of differentiation of urine films taken from healthy donors and albuminuria patients by means of statistical analysis of such distributions are determined. The operational characteristics (sensitivity, specificity, and accuracy) of the Mueller-matrix reconstruction method of the optical anisotropy parameters are defined.