Adriana Rodrigues Passos

Caracterização morfológica e molecular de genótipos de mangaba

The objective was to assess the genetic diversity among 25 Mangaba genotypes in the Iramaia-Chapada Diamantina region, Bahia, using morphological markers and RAPD markers. The following measurements were performed in mature fruits: longitudinal (LD) and transverse (TD) diameter, fruit (FM), seed (SM) and the pulp (PM) mass, pulp yield (% P) and the count seed number (SN). The chemical characters were evaluated in mature fruit: pH, acidity (Ac), total soluble solids (TSS), ascorbic acid (AA), total sugar (TS) reducing sugar (RS), non-reducing sugar (NRS) and total soluble solids /acidity (TSS/Ac). For amplification were tested 51 random primers. The average results of the measurements were LD (37.03 mm), TD (34.34 mm), FM (24.45 g), SM (2.42 g), PM (22.03 g), and SN (11.14). For chemical evaluations were checked the average results: % P (88.44%), AA (80.23 mg100g-1), Ac (0.96%), pH (3.67), TSS (14.15 oBrix) TS (9.26 mg100g-1), RS (5.13 mg100g-1), NRS (4.13 mg100g-1) and TSS/Ac (14.56 mg100g-1). Eight random primers were selected for having more intense bands and six (OPA 08, OPB 12, OPB 19, OPH 15, OPH 18, OPH 19) were sufficient to generate 28 polymorphic bands. Among the morphological markers, FM and PM were the most effectively contributed to divergence. The formation of six and seven groups for molecular markers and morphological, respectively, indicated the presence of genetic variability in the population. .

Yield adaptability and stability of semi-prostrate cowpea genotypes in the Northeast region of Brazil by REML/BLUP

Cowpea is grown in the various soil and climatic conditions of the Northeast region of Brazil. Thus, selecting and developing cultivars with high yield, stability, and adaptability for this region is necessary due to the genotype × environment interaction. The objective of this work was to select cowpea lines of semi-erect plant simultaneously for high yield, adaptability, and genotypic stability in the Northeast region of Brazil by the REML/BLUP procedure. Twenty semi-erect genotypes—15 lines and five cultivars—were evaluated in 37 environments of the Northeast region from 2013-2015. The experiments were carried out under rainfed conditions in a randomized complete block design with four replications. The adaptability and genotypic stability were evaluated by the REML/BLUP procedure. The environmental variance was the largest factor in the phenotypic variance and the genotype × environment interaction was complex-type, with a grain yield ranging from 277 kg ha-1 (Serra Talhada PE, 2015) to 2,845 kg ha-1 (Sao Raimundo das Mangabeiras MA, 2013), and an overall mean of 1,342 kg ha-1. According to the Harmonic Mean of Relative Performance of Genetic Values (HMRPGV) estimates, the lines MNC04-795F-153 and MNC04-795F-159 were those that simultaneously had high yield, adaptability, and genotypic stability, and can be recommended and grown with greater probability of success in the evaluated environments in the Northeast region of Brazil.