Amaury José Teixeira Nigro

Estudo morfológico de aderências peritoneais induzidas pela rifamicina, em ratos

The intraperitoneal use of rifamycin s.v. and its influence in postoperative peritoneal adhesions occurrence was analysed in a ninety male rats experimental study. The control group was formed by 45 animals subjected to laparotomy and instillation of 2mL of saline solution with rhythmic digital movements in the four quadrants of the peritoneal cavity for 2 minutes. After this procedure, the remaining saline solution was aspirated with a syringe, followed by immediate laparorrhaphy. The experiment group (n=45) was submitted to a similar procedure with instillation of a solution of rifamycin s.v. 500mg diluted in 500mL of a saline vehicle, according to the same movement used in the control group. The animals of both control and experiment groups were distributed in three subgroups with equal number (n=15), according to the euthanasia period, which was 7, 14 and 21 days postoperative. Peritoneal adhesions were quantified in the euthanasia periods, using a method described by MORENO-EGEA, AGUAYO, ZAMBUDIO and PARRILLA (1993). The experiment group presented a rate of adhesion significantly high as compared to the control group in all subgroups. This difference was higher in animals with an euthanasia period of 21 days.

Viabilidade celular da mucosa do intestino delgado de ratos, após correção de choque hipovolêmico com solução de NaCl 7,5%

PURPOSE: Study the effect of the volemic correction with different solutions, in the mucous of the small bowel in rats. METHODS: Were used 120 rats Wistar (Rattus norvegicus albinus), males, adults, seemingly healthy, with individual weight varying between 310 and 410g, originating from of the Instituto Evandro Chagas of Belem of Para, submitted to an adaptation period of 15 days, receiving water and ration ad libitum, during the role experiment. For the research, ten animals were distributed, in groups and subgroups as following: Standard group (S), Shock group (Sh), Physiological Solution group (PS) and Hypertonic Solution Group (HS). The groups were divided in subgroups with 10 animals each, in agreement with the day of postoperative (DPO) foreseen for the euthanasis of the animals, (1st, 3rd or 7th DPO), being after this, picked material for cellular viability in every animals. RESULTS: The group PS took less quantity viable cells. CONCLUSION: The volemic correction with chloride of sodium solution at 7.5%, when compared the correction with chloride of sodium at 0.9% (isotonic solution), took the maintenance of larger amount of viable cells, in the small bowel in rats.

Estudo comparativo do tratamento de ferimento de colon de coelho com reparo seroso da parede de jejunum e sutura primária

The present research aimed to compare the repair of colon wound using both the jejunal wall and the primary suture . Thirty young rabbits white New Zeland were used, weighing about 3,2 Kg. and were born and raised at the University of Maringas Experimental farm. The animals were deeply anaesthetized and then submited to a surgery during wich two similar wounds were made in unprepared colon . An instrument created for this purpose was used. The first wound was made 2 cm away from the ceco , and the second one was made 20 cm from the first wound in the caudal location. The wounds were repaired using either the jejunal serosal wall or the primary suture. In both techniques were used separated stitches , made by monofilament poliglecaprone 4-0. The animal was used to control itself. The animals were submited to euthanasia either in the 7th or in 30th day after surgery, at random.Then a macroscopic and microscopic study was made. This research has come to prove that the use of jejunals serosal wall is available. This techinique has shown better results than the primary suture : neither were there oclusion nor fistula. Besaid , the histologic studies have proved the existence of neomucous epithelium on the 7th day after surgery.

ENXERTO DE FÁSCIA LATA AUTÓGENA EM FERIMENTOS PENETRANTES PROVOCADOS NO ESÔFAGO CERVICAL DE CÃES

The aim of this study was to study the efficiency of autogenus graft of fascia lata to treat experimental wounds at the cervical level of esophagus of dogs. Two operative procedures were used in forty dogs in order to correct the injuries and compared among the groups. The standard area of the injury ranged was 3,12 cm square. The animals were divided in two groups of 20 each, so-called Group A ( suture group ) and Group B ( graft groups ). Group A and B were subdivided in two another group, so-called Group A1 A2 and B1,B2 with 10 animals each one with the pourpose of reoperation at seventh and thirtieth past operatives days. The Group A, so-called Control Group was undergone a techinique of extramucous suture with simple points of nylon on transversal plan of esophagus axis. On Group B, so-called Experimental Group it was employed autogenos graft of fascia lata in order to oclud the penetrating injury. The grafts was sutured on the esophagus wal by extramucous technique with the nylon . Macroscopic and microscopic results were compared among the two groups by invariate analysis. No statistical significant differences were observed among the groups besides the animals of Group A had showed a good clinical behavior and lower incidence of fistulae. However on Group B ( undergone a fascia lata graft ) had shouwed five cases of fistulae. In conclusion, the fascia lata not appear to be useful for treat penetrating wounds at the cervical level of esophagus in dogs.

Uso do adesivo de fibrina na anastomose esôfago-esofágica cervical, em cães

BACKGROUND: The aim of this research was to study the effects of the use of fibrin adhesive with the surgical technique of submucosa invagination, in esophageal anastomosis. METHODS: Thirty two dogs operated on for esophageal anastomosis, were distributed in two groups: group I with encircling suture of twelve stitches, and group II with encircling suture of four stitches and fibrin sealant. The anastomosis was evaluated in the 7th and 14th post-operative day. Weight evolution, stenosis rate, presence of dehiscence and fistulae, presence of secretion around the anastomosis, presence of interstitial liquid, protean matrix, number of cells, fibroblasts, collagen fibers and the hydroxyproline concentration in the anastomosis were analysed. RESULTS: The stenosis rate was lower in the group I in the 7th post-operative day. The incidence of fistulae was significant in group II on the 7th day, as well as the presence of dehiscence, serosa secretion and suppurative secretion at the 7th and 14th day. Concerning hydroxyproline tissue concentration there was no statistical difference among the groups. The fibroblasts and collagen fibers were more numerous in group II at 14th day. There were four deaths in animals of group II. CONCLUSION: We concluded that anastomosis performed by submucosa-mucosa invagination with fibrin adhesive presented results less satisfactory than anastomosis performed with twelve encircling stitches.

Gangliosideos - estudo do colágeno e da resposta inflamatória no processo cicatricial

Objetivo: Investigar o efeito dos gangliosideos sobre a infiltracao sequencial de leucocitos e fibroblastos durante o processo de cicatrizacao usando um modelo de cicatrizacao da pele em ratos. Metodo: Foram utilizados 12 ratas EPM - 1 Wistar, com peso medio de 200 gramas e 4 meses de idade. Os animais procederam do Centro de Desenvolvimento de Pesquisa Experimental em Medicina e Biologia e foram mantidos por 5 dias para adaptacao no bioterio setorial da disciplina de Tecnica Operatoria e Cirurgia Experimental da UNIFESP-EPM, recebendo agua e racao propria para a especie. O protocolo anestesico utilizado foi uma associacao de Cetamina (60mg.kg-1) e Xilazina (10mg.kg-1), por via intramuscular. Em seguida, realizava-se uma incisao longitudinal com 7 cm de extensao, na regiao dorsal paravertebral, interessando pele e tela subcutânea que foi fechada com pontos separados, com fio de prolene 7-0 e agulha triangular. As ratas foram distribuidas em dois grupos a saber: grupo experimento, que recebeu 3mg.kg-1.dia-1 de gangliosideos, e um grupo controle, que recebeu veiculo, ambos por via intramuscular durante 14 dias consecutivos. No 7o e 14o dias de pos-operatorio foram ressecados fragmentos da pele e tela subcutânea para analise histologica, com a coloracao de Tricromio de Masson e Hematoxilina - Eosina. Resultados: As amostras apresentaram a mesma quantidade de colageno em ambos os grupos mostrando que nao houve inibicao dos fibroblastos. Entretanto, a infiltracao leucocitaria foi retardada no grupo experimento quando comparado ao grupo controle. Conclusao: A alteracao encontrada no processo cicatricial foi devida a um retardo na resposta inflamatoria e nao a uma inibicao fibroblastos.

Estudo dos efeitos das ressecções colônicas parciais em ratos

UNLABELLED In order to study the effects of partial colectomy, 30 rats Wistar were divided into three groups: GI and GII had the cecun and the ileo cecal valve resected and proximal colectomy was performed in GI and distal in GII. GIII had distal colectomy without cecum resection. The length of the remain colon was of 5 cm in all groups. Colostomy was performed in GI, GII and GIII. Parameters evaluated: body weight, fecal composition concerning to water, lipids and proteins on preoperative time and on the 10th, 20th, 30th, 40th, 50th and 60th days of postoperative time. All statistical tests were conducted at a 5% two-sided risk level. The evaluation was made by analysis of variance techniques. CONCLUSIONS Concerning to body weight, there is no significant difference on the late postoperative time in any group or when comparing the three groups on this same period. On late postoperative time, GI and GII had a large amount of fecal water, lipids and proteins when compared to the preoperative time and to GIII. On the 60th day theres no significant difference on the quantity of fecal water when comparing the three groups and the same occurs on the 50th day when considering the quantity of fecal proteins.In order to study the effects of partial colectomy, 30 rats Wistar were divided into three groups: GI and GII had the cecun and the ileo cecal valve resected and proximal colectomy was performed in GI and distal in GII. GIII had distal colectomy without cecum resection. The length of the remain colon was of 5 cm in all groups. Colostomy was performed in GI , GII and GIII. Parameters evaluated: body weight, fecal composition concerning to water, lipids and proteins on preoperative time and on the 10th, 20th, 30th, 40th, 50th and 60thdays of postoperative time. All statistical tests were conducted at a 5% two-sided risk level. The evaluation was made by analysis of variance techniques. Conclusions: Concerning to body weight, there is no significant difference on the late postoperative time in any group or when comparing the three groups on this same period. On late postoperative time, GI and GII had a large amount of fecal water, lipids and proteins when compared to the preoperative time and to GIII. On the 60th day theres no significant difference on the quantity of fecal water when comparing the three groups and the same occurs on the 50th day when considering the quantity of fecal proteins.

INVESTIGAÇÃO DO USO DE HEPARINIZAÇÃO REGIONAL DURANTE ISQUEMIA ARTERIAL TEMPORÁRIA EM COELHOS

The purpose of this study is to evaluate the effect of the regional heparinization during temporary arterial ischemia performed in rabbits. The evaluated parameters served for measuring were activated partial thromboplastin times (aPTT) as well as the presence or absence of thrombi or endothelial edema in the analyzed vessels. Forty New Zealand rabbits were utilized. They were distributed into two groups of twenty animals. After dissection and repair of the left illiac artery, an arteriotomy was carried out and then a polyethylene catheter was fitted in so that heparin solution could be injected into the experimental group and physiological serum into the control group. The aPTT was measured at the beginning of the experiment and after ninety minutes of ischemia. There were no significant differences found from the initial values to the end of the experiment. Throughout microscopic evaluation the presence of thrombi in the vessels of the animals from both groups was not found. Intimal thickening as well as disruption of the middle layer regarded as endothelial edema ocurred in only one animal from the control group, however it was not considered a significant data. It was concluded that the injection of heparin (60 UI/kg weight) did not provoke significant alterations in the systemic coagulation of the rabbit. Furthermore, in the absence of endothelial lesion thrombosis of the analyzed vessels did not occur, neither with nor without the utilization of heparin.

ESTUDO COMPARATIVO ENTRE DIFERENTES TIPOS DE REVESTIMENTO INTERNO DE ANASTOMOSES ESOFÁGICAS EM CÃES

The aim of this work was to compare, in a prospective and randomized way, the use of two suture techniques: one using a PENROSE drain as an internal lining and the other using submucosal-mucosal invagination in the esophagus-esophagical cervical anastomosis. Thirty-two male dogs with an average weight between 8 and 10 Kg were distributed in two groups of 16 animals. Each group was divided into 2 subgroups based on post-operative time periods: 7 and 14 days. In each group, the morbidity and macroscopic and microscopic alterations caused by anastomosis were evaluated. No fistula and no death were observed in the pos-operative period. All animals from PENROSE drain as an internal lining group presented dysphagia by at least 7 days, while 4 animals presented transitory dysphagia for only 2 days in the invagination suture group. Optical microscopic analysis revealed deficiency of regeneration in the submucous and mucous tunics in the anastomosis with prosthesis. The results permitted to conclude that the esophagus-esophageal cervical anastomosis using a PENROSE drain as an internal lining is less efficient that executed by submucosal-mucosal invagination.

Anastomose esôfago-esofágica cervical por invaginaçäo versus a em plano único extramucoso, no cäo

O objetivo e verificar a eficacia da anastomose esofago-esofagica por invaginacao quando comparada a executada em plano unico extramucoso. Utilizou-se 60 caes, sadios, sem raca deficida, de ambos os sexos, com peso medio de 15kg. Foram separados em dois grupos iguais; no GRUPO I sera feita anastomose por invaginacao e, no GRUPO II, a em um plano unico extramucoso. Cada grupo foi subdividido em 3 subgrupos iguais, e para cada um deles foi estabelecido um determinado periodo de observacao pos-operatorio (PO) de 7, 14 e 30 dias, respectivamente. Retirou-se no GRUPO I as tunicas adventicia e muscular para formar um tubo de submucosa-mucosa de 2 centimetros de comprimento que foi mantido em continuidade com o coto cervical proximal e no GRUPO II foram extirpadas todas as tunicas do esofago cervical com a mesma extensao ressecada no grupo I. As suturas para aproximar os cotos esofagicos foram todas feitas com fio de polipropileno 4 zeros em um plano unico extramucoso, mas nos animais do GRUPO I foi invaginado, previamente, o tubo de submucosa-mucosa no interior do coto esofagico caudal. Avaliou-se a incidencia de complicacoes pos-operatorias imediatas e tardias e as caracteristicas histologicas da cicatrizacao de cada tipo de anastomose. A analise estatistica dos dados coletados permitiu verificar que na anastomose esofago-esofagica cervical por invaginacao nao ocorreu fistula ao nivel da sutura em nenhum cao da serie e verificou-se estenose, muito leve, em apenas um animal. Por outro lado, os caes submetidos a anastomose em plano unico extramucoso apresentaram 13% de fistulas e 30% de estenose. A cicatrizacao das estruturas da parede esofagica ao nivel da anastomose foi significantemente melhor na anastomose por invaginacao do que na executada em um plano unico extramucoso. Pode-se concluir que a anastomose esofago-esofagica cervical por invaginacao e mais eficaz do que a feita em plano unico extramucoso.