Ana Yasha Ferreira de La Salles

Obtenção de oócitos em cadelas e gatas submetidas a ovariosalpingohisterectomia

The use of modern techniques for application in the field of reproduction grows more and more in veterinary medicine and therefore studies are needed to evaluate the efficiency of these techniques. Due to the cortical location of the follicles within the ovary, Slicing is the most adequate technique to obtain canine oocytes, which presents better results in relation to the amount of oocytes collected when compared to other techniques. The aim of this study was to verify the quality of oocytes collected from ovaries of bitches and cats with different ages submitted to the Salpingo Ovary Hysterectomy (OSH) procedure at the Veterinary Hospital of the Federal University of Campina Grande. The oocytes were classified according to their quality in relation to the morphology of the cumulus oophorus complex. The age and estrus stage in which bitches and cats were at the time of collection did not influence the quality and quantity of oocytes collected.

Diagnóstico por tomografia computadorizada da extrusão de disco intervertebral em paciente geriatra: Relato de Caso

Intervertebral disc disease (IVDD) is the most common cause of spinal cord compression in dogs, resulting in neurological problems, which can be classified into two types: Hansen type I (disc extrusion) and Hansen type II (disc protrusion), pressing the nerves of the marrow causing pain, ataxia, paralysis and paraplegia. The indicated treatment should be based on the degree of injury, and may be the clinical treatment associated with physical therapy, for less severe cases, based on the successes in the recovery of the condition, reported in the literature. The objective of this study was to report the efficiency of computed tomography as a complementary tool for conclusive diagnosis of DDIV and the success of clinical and physiotherapeutic treatment for this disease. The animal in question was an 11-year-old poodle that presented ataxia and motor

Ovary and vaginal epithelium dynamics during the estrous cycle in Dasyprocta prymnolopha Wagler, 1831: ultrasound and cytological examinations

The objective of the present study was to monitor and characterize morphological alterations in ovaries of agouti (Dasyprocta prymnolopha), reared in captivity, by using abdominal ultrasonography. All animals underwent daily vaginal cytological examination to identify the current cycle phase. For each phase of the estrous cycle, ultrasound examinations were carried out to identify and describe the morphology of both ovaries. Topographic parameters in an ultrasound window were established to locate the ovaries. The agouti estrous cycle lasted an average of 29.94 ± 6.77 days. During vaginal cytology examinations, all cell types were identified, and each phase of the estrous cycle was established by cell counts. No significant alterations were observed in the assessed ovarian morphometry measurements. In 75% of the animals examined, ovarian follicle presence was observed in the proestrus phase.

Anatomic Bases for Brachial Plexus Block of Capuching (Sapajus libidinosus)

Background: In captivity, capuchin monkeys compete for space and rank. Fights can result in traumas, especially to the limbs, requiring interventions that are often outpatient. Local anesthesia as a tool in these procedures, as an aid to chemical restraint, is very relevant for small outpatient surgeries, or even for pain relief. Knowledge of peripheral nerve anatomy is essential to perform local anesthesia. Thus the objective of the present study was to determine, by anatomical studies of the brachial plexus region, the best access pathways for anesthetic blocking of the nerve. Materials, Methods & Results: Seven adult capuchin monkeys (Sapajus libidinosus) were used, weighing 2-3 kg, fixed and preserved in formaldehyde aqueous solution at 10%. In five of these animals the supraclavicular, infraclavicular and axillar regions were dissected to visualize the muscles, clavicle and bracchial plexus nerves. An analogical pachymeter was used to measure the depth of the plexus in relation to the cranial and caudal clavicle face and axillary fossa, comparing the length of two hypodermic needles (13x4.5 mm and 15x5 mm). Simulation of the anesthetic block was tested in two animals: before dissecting an acrylic varnish solution was injected using a syringe and 13x4.5 mm needle in the supraclavicular, infraclavicular regions and axillary fossa. To assess the positioning points of the syringe, dissection was performed and the varnish perfusion in the plexus was observed. For the anesthetic block in the supraclavicular region the dorsal median of the clavicle with a 95o deltoclavicular angle with the needle perpendicular to the skin was taken as point of reference. In the infraclavicular the reference point was the caudal face of the median clavicle with an 80o deltoclavicular angle. In the axillar region, with the limb at 90o, the syringe was positioned perpendicular to the axillar at the height of the mid portion of the thorax. The mean and standard deviation of the skin-brachial plexus distance for the supraclavicular, infraclavicular and axillar techniques were, respectively, 1.76 ± 0.1387 cm, 1.12 ± 0.239 cm and 1.59 ± 0.365 cm. These data showed the viability of executing the anesthesia technique by three access pathways. However, in the supraclavicular access pathway in the anesthetic simulation with the 13x4.5 mm hypodermic needle, the stain diffused to the plexus, showing, when compared with the 1.76 cm mean skin- plexus distance a safe distance to prevent the needle from perforating the nerve. Discussion: The techniques reported in the human literature for brachial plexus block presented a series of complications, with incomplete blocks and hemorrhages when the axillar access pathway was used and presented risk of pneumothorax in the supraclavicular. In the capuchin monkey the supraclavicular access pathway in anesthetic simulation with 13x4.5mm hypodermic needle showed a safe distance for the needle not to perforate the nerve, so that the use of this needle could be indicated in 2-3 kg animals. However, hypodermic needles are not recommended for use in this area because of the risk of perforating the subclavicular artery close to the plexus. As an alternative some anesthesiologists use the infraclavicular access pathway with atraumatic needles recommended for brachial plexus block, with relative success and fewer complications. Although the supraclavicular region showed the best depth in relation to the technique used here, anesthetic tests should be made to confirm the efficaciousness of executing the brachial plexus anesthetic technique in capuchin monkeys using atraumatic needles for nerve block.

Anatomia do cone medular do cachorro-do-mato (Cerdocyon thous) aplicada à via epidural de administração de fármacos

In order to determine the best location for performing the technique of epidural access either to epidural anesthesia, myelography scans, spinal tap, among others, this study aimed to describe the anatomy of the conus of the crab eating fox (Cerdocyon thous), particularly its esqueletopia. For the realization of this work six adult animals of both sexes were dissected, withdrawing from the muscles of the lumbar and sacral vertebrae column to identify, removing the muscles of the lumbar and sacral vertebrae column to identify which were sectioned in their vertebral arches to expose the spinal cord. Medullary cone was identified, measured with a pachymeter and identified its esqueletopia. In all

Anatomical description of the main vessels for venipuncture in the black-striped capuchin monkey (Sapajus libidinosus, Silva Junior, 2002)

The scarcity of data on the anatomy of Sapajus libidinosus has impeded the execution for appropriate veterinary treatment. The objective of this study was to describe the main peripheral veins of the capuchin monkey, used in venipuncture and indicate the best access route for venipuncture procedures.