Danilo José Ayres de Menezes

Análise qualitativa do estabelecimento da espermatogênese em cutias (Dasyprocta aguti) criadas em cativeiros

A determinacao do estabelecimento da puberdade e bastante estudada em animais domesticos e roedores, no entanto, sao escassas as pesquisas com a finalidade de estabelecer parâmetros para a biologia reprodutiva em cutias. Foram utilizadas 31 cutias machos da especie Dasyprocta agouti, oriundas da Universidade Federal do Piaui, Estado do Piaui, e da Escola Superior de Agricultura de Mossoro, Estado do Rio Grande do Norte. Imediatamente apos a orquiectomia foram retirados fragmentos e estes foram processados histologicamente, os tecidos foram corados com hematoxilina-eosina e analisou-se os parâmetros seguintes: aspectos de luminacao dos tubulos seminiferos; presenca de espermatocitos primarios; presenca de espermatides e formacao dos primeiros estagios do ciclo do epitelio seminifero (CES) segundo o metodo da morfologia tubular. O periodo desde o nascimento ate os cinco meses de idade correspondeu a fase impubere; dos seis aos oito meses de idade a fase de transicao da pre-puberdade a puberdade; dos nove aos dez meses de idade a fase da puberdade; e dos doze aos quartoze meses de idade a fase da pos-puberdade. A puberdade da cutia (Dasyprocta aguti), ocorreu em animais a partir dos sete meses de idade, e o estabelecimento da puberdade foi constatado em todos os animais estudados aos nove meses de idade.

Estudo morfológico e morfométrico do ovário de cutias (Dasyprocta aguti, Linnaeus, 1766)

Foram estudados 24 ovarios pertencentes a 13 cutias adultas (em dois exemplares pesquisou-se apenas o ovario esquerdo), sendo 7 gestantes e 6 nao gestantes, provenientes do Nucleo de Estudos e Preservacao de Animais Silvestres da Universidade Federal do Piaui. Os orgaos foram observados in loco para descricao de sua topografia, mensurados, submetidos a cortes histologicos e observados em Microscopio Optico. Os ovarios apresentam-se de forma elipsoide, achatados dorsoventralmente, situando-se na regiao sub-lombar, caudalmente aos rins, com pequenas areas transparentes em sua superficie externa. A margem mesovarica e a face lateral estao cobertas pelo mesossalpinge (bolsa ovarica). O ovario direito, apresenta em media: peso - 0,082g; comprimento - 0,83cm; largura - 0,49cm e espessura - 0,24cm; o ovario esquerdo: peso - 0,058g; comprimento - 0,74cm; largura - 0,45cm e espessura - 0,23cm. Histologicamente, constam de epitelio de revestimento cubico simples, cortex periferico e medula central, constituida basicamente por tecido conjuntivo frouxo entremeado por vasos sanguineos. Nas femeas gestantes foram observados de dois a tres grandes corpos luteos centrais e muitos outros menores perifericos; nas nao gestantes os corpos luteos sao menores e mais numerosos. O cortex e rico em tipos celulares de natureza conjuntiva e em foliculos em diferentes estagios de amadurecimento, os quais migram da margem mesovarica para a extremidade tubarica a medida que aumentam de tamanho. Conclui-se que os ovarios da cutia, macro e microscopicamente, seguem o padrao observado nos demais roedores sexualmente ativos.

Segmentos anátomo-cirúrgicos arteriais do rim de cutia (Dasyprocta prymnolopha)

Twenty pairs of agouti (Dasyprocta prymnolopha Wagler, 1831) kidneys were studied to describe the arterial anatomical-surgical segments. The renal arteries were injected with stained acetate vinyl, followed by procedures of acid corrosion in order to obtain vascular casts. It was found that the renal artery is always single and bifurcated into ventral and dorsal sectorial arteries. The sectorial arteries reached the kidneys (100% of the cases) through the hilus. These vessels gave origin to segmental branches responsible for kidney irrigation. At the right kidney, the ventral sectorial arteries gave origin to 3 (60% of the cases), 4 (35%) and 5 (5%) segmental branches; the dorsal sectorial arteries gave origin to 3 (30%), 4 (45%), 5 (20%) and 6 (5%) segmental arteries separated by a vascular sector. At the left kidney, the ventral sectorial arteries originated 2 (10%), 3 (55%) or 4 (35%) segmental branches; the dorsal sectorial arteries gave origin to 3 (25%), 4 (50%) and 5 (25%) segmental branches. Based on the arterial distribution of agouti kidneys, independent sections and arterial segments were found, so that it is possible to accomplish partial kidney resection surgery.

Influence of the bipartite scrotum on the testicular and scrotal temperatures in goats

The influence of the scrotal bipartition and of the year period on the scrotal-testicular thermal regulation was evaluated in male goats raised in Piaui State, Brazil. Eighteen male goats at mating age were accomplished in this study and arranged into three Groups (6 animals each) obeying the classification as goats presenting no scrotal bipartition (Group I), goats showing scrotal bipartition at 50% of the testicular length (Group II), and goats with more than 50% of scrotal bipartition (Group III). The scrotal, testicular and spermatic funiculi temperatures were evaluated invasively with the aid of a digital thermometer and non-invasive with a pyrometer in the proximal, medial and distal portion. The data were acquired during the dry (October-November) and rainy (February-March) period of the year, measured in two shifts: morning (6h00-7h00) and afternoon (14h00-15h00). The results were submitted to variance analysis (ANOVA) following the SNK test for average comparison (p 50%). It is possible to conclude that with the experimental conditions applied on this study, the level of scrotal bipartition and the climatic conditions interfere with the scrotal-testicular thermal regulation in goats.

Testicular arteries systematization based on different levels of scrotal configuration in caprines

The objective of this study was to describe the distribution of testicular arterial vessels in caprines with different degrees of scrotal division. Scrotal configurations were classified as follows; Group I: scrotum without bipartition; Group II: scrotum showing a ventral division until the middle of the testis; and, Group III: scrotal separation extended beyond the middle of the testis. A colored vinyl acetate solution was injected into 60 testicular arteries (30 pairs). After, the samples were submitted to corrosion cast method to obtain tubular vascular models for macroscopic analysis. We observed that the testicular arteries emerge from the abdominal aorta, cross the inguinal canal to become coiled and involved by the pampiniform plexus. Close to the caudal extremity of the testis, the arteries become divided in cranial and caudal branches that emit collateral vessels to originate emerging branches. Testicles with intermediate level of scrotal division (group II) showed a smaller amount of those branches than the others, being the most populated the ventrolateral and the dorsolateral quadrants. The origin, distribution and localization of the testicular arteries did not show any differences related to the degree of scrotal division in caprines.

Epidural anesthesia in capuchin monkeys (Sapajus libidinosus)

Many primates are kept in breeding for scientific or conservation purposes, but much of the clinical care is represented by trauma. To provide more effective interventions in some of these cases, the present study aimed to evaluate the technique of local anesthesia through the epidural space in capuchin monkeys (Sapajus libidinosus).

Anatomia do cone medular aplicada à via epidural de administração de fármacos em macacos-prego (Sapajus libidinosus)

Com este estudo objetivou-se descrever a topografia do cone medular do macaco-prego (Sapajus libidinosus) a fim de fornecer suporte para que a realizacao de procedimentos anestesicos, bem como exames de mielografia e coleta de liquor, dentre outros procedimentos que utilizam a via epidural. Para tanto foram dissecados oito animais, sendo seis machos e duas femeas, de diferentes faixas etarias. Rebateu-se a pele para retirada da musculatura da regiao dorsal, exposicao de toda a coluna vertebral e identificacao das vertebras lombares e sacrais. Para estabelecer o final da medula espinhal e medir o comprimento do cone medular, foi aberto todo o canal vertebral lombossacro, seccionando-se lateralmente os arcos vertebrais. Em seguida a duramater foi seccionada para visualizacao do cone medular e observacao da relacao topografica deste com as vertebras. Todos os animais apresentaram cinco vertebras lombares e tres vertebras sacrais. As vertebras se apresentaram, de forma geral, muito proximas e com os processos espinhosos bem desenvolvidos e direcionados em sentido cranial. O cone medular dos macacos-prego situou-se entre as vertebras L2 e L5, com a base localizando-se com maior frequencia na altura da vertebra L3, enquanto o apice em L4. O comprimento corporal (espaco interarcual occiptoatlântico ate o espaco interarcual sacrocaudal) variou de 22,9 a 31,8cm, com media de 27,44 ±3,1cm enquanto que comprimento do cone medular variou de 1,70 a 3,51cm, com media de 2,47 ±0,57cm. Nao houve correlacao entre o tamanho do corpo e o comprimento do cone medular (r = 0,212). Conclui-se que apesar das variacoes do comprimento e posicionamento do cone medular, o seu apice nao ultrapassa a articulacao lombossacral, tornando seguro o acesso ao espaco epidural por esta via.

Obtenção de oócitos em cadelas e gatas submetidas a ovariosalpingohisterectomia

The use of modern techniques for application in the field of reproduction grows more and more in veterinary medicine and therefore studies are needed to evaluate the efficiency of these techniques. Due to the cortical location of the follicles within the ovary, Slicing is the most adequate technique to obtain canine oocytes, which presents better results in relation to the amount of oocytes collected when compared to other techniques. The aim of this study was to verify the quality of oocytes collected from ovaries of bitches and cats with different ages submitted to the Salpingo Ovary Hysterectomy (OSH) procedure at the Veterinary Hospital of the Federal University of Campina Grande. The oocytes were classified according to their quality in relation to the morphology of the cumulus oophorus complex. The age and estrus stage in which bitches and cats were at the time of collection did not influence the quality and quantity of oocytes collected.

Infestação parasitária por Spirura sp. em Callithrix jacchus (Linnaeus, 1758): relato de caso

The knowledge of gastrointestinal parasites in primates is important for the management of the population of monkeys and for the maintenance of the health of the people, since they are common animals in captivity. The study reports a case of parasitism by Spirura sp. in an adult Common marmoset found in the Zona da Mata region of Paraíba state. Spirura sp. was identified in the final portion of the esophagus of a Sagittarian corpse that was donated for anatomical study by the Paraíba Wild Animal Triage Center.

Diagnóstico por tomografia computadorizada da extrusão de disco intervertebral em paciente geriatra: Relato de Caso

Intervertebral disc disease (IVDD) is the most common cause of spinal cord compression in dogs, resulting in neurological problems, which can be classified into two types: Hansen type I (disc extrusion) and Hansen type II (disc protrusion), pressing the nerves of the marrow causing pain, ataxia, paralysis and paraplegia. The indicated treatment should be based on the degree of injury, and may be the clinical treatment associated with physical therapy, for less severe cases, based on the successes in the recovery of the condition, reported in the literature. The objective of this study was to report the efficiency of computed tomography as a complementary tool for conclusive diagnosis of DDIV and the success of clinical and physiotherapeutic treatment for this disease. The animal in question was an 11-year-old poodle that presented ataxia and motor