Arief Rahman Hakim
Gadjah Mada University
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Publication
Featured researches published by Arief Rahman Hakim.
Asian Pacific Journal of Tropical Medicine | 2013
Arief Nurrochmad; Supardjan A. Margono; Sardjiman; Arief Rahman Hakim; Ernawati; Erna Kurniawati; Erva Fatmawati
OBJECTIVE To investigate the hepatoprotective and antioxidant activity of pentagamavunon-0(PGV-0) against CCl4-induced hepatic injury in rats. METHODS The groups of animals were administered with PGV-0 at the doses 2.5, 5, 10, and 20 mg/kg b.w., p.o. once in a day for 6 days and at day 7 the animals were administrated with carbon tetrachloride (CCl) (20%, 2 mL/kg b.w. in liquid paraffin (i.p.). The effect of PGV-0 on serum transaminase (SGPT), alkaline phosphates (ALP) and total bilirubin were determined in CCl4-induced hepatotoxicity in rats. Further, the effects of PGV-0 on glutathione (GSH) content, catalase (CAT) and NO free radical scavenging activity also were investigated. RESULTS The results demonstrated that PGV-0 significantly reduced the activity of SGPT, serum ALP and total bilirubin in CCl4 induced rat hepatotoxicity. PGV-0 has effect on the antioxidant and free radical defense system. It prevented the depletion level of GSH and decrease activity of CAT in CCl4-induced liver injury in rats. PGV-0 also demonstrated the free radical scavenger effects on NO free radical scavenging activity with ES value of 32.32 μM. CONCLUSION All of our findings suggests that PGV-0 could protect the liver cells from CCl4-induced liver damages and the mechanism may through the antioxidative effect of PGV-0 to prevent the accumulation of free radicals and protect the liver damage.
Indonesian Journal of Chemistry | 2017
Dion Notario; Sudibyo Martono; Zullies Ikawati; Arief Rahman Hakim; Fathul Jannah; Endang Lukitaningsih
To conduct a bioequivalence study for a copy product of levofloxacin (LEV), a simple and validated analytical method was needed, but the previous developed methods were still too complicated. For this reason, a simple and rapid high performance liquid chromatography method was developed and validated for LEV quantification in human plasma. Chromatographic separation was performed under isocratic elution on a Luna Phenomenex ® C18 (150 × 4.6 mm, 5 µm) column. The mobile phase was comprised of acetonitrile, methanol, and phosphate buffer 25 mM that adjusted at pH 3.0 (13:7:80 v/v/v) and pumped at a flow rate of 1.5 mL/min. Detection was performed under UV detector at wavelength of 280 nm. Samples were prepared by adding acetonitrile and followed by centrifugation to precipitate plasma protein. Then followed successively by evaporation and reconstitution step. The optimized method meets the requirements of validation parameters which included linearity (r = 0.995), sensitivity (LLOQ and LOD was 1.77 and 0.57 µg/mL respectively), accuracy (%error above LLOQ ≤ 12% and LLOQ ≤ 20%), precision (RSD ≤ 9%), and robustness in the ranges of 1.77-28.83 µg/mL. Therefore, the method can be used as a routine analysis of LEV in human plasma as well as in bioequivalence study of LEV.
INDONESIAN JOURNAL OF PHARMACY | 2005
Agung Endro Nugroho; Arief Rahman Hakim
Drug interaction phenomenon, between drugs on drug and ford (fruit, vegetables on others), can happen in drug therapy of a disease due to the existence of compounds other than the drug (fruits, vegetables or drink). This interaction can alter and affect the action and effect of the drug. The aim of the research is to study an interaction between betle leaf (a vegetable ) and propranolol, which is a beta-blocker with a high hepatic extraction ratio, in male white rat. The study was conducted by employing a completely randomized design in male wistar inbreed rat (aged 2-2.5 months, 150-250 g). The animals in group I were given propranolol with an oral single dose of 7.5 mg/kg BW (control group) and in group II were given pretreatment with betle leaf juice dose of 17 mL/kg BW at an hour prior propranolol administration. After collected at some certain times, the drug concentration on bloods were analyzed with a Spectropflurometer. The results shown that pretreatment of betle leaf juice did not affect the absorption rate constant (Ka) significantly (P>0.05), and increase the total clearence (ClT) and volume of distribution in steady state (Vdss) significantly (P<0.05). The increasing of both ClT and Vdss caused alteration of the secondary pharmacokinetics parameters of propranolol and these derivates significantly (P<0.05) except the mean residence time value (MRT). This it is concluded that the pretreatment of betle leaf juice with the dose of 17 mL/kg BW affect the pharmacokinetics of propranolol or decrease the propranolol concentration in blood. Key words : propranolol, betle leaf, pharmacokinetics and drug interaction
Archive | 2002
Mochammad Samhoedi Reksohadiprodjo; Henk Timmerman; Sardjiman Dummy; Supardjan A. Margono; Sudibyo Martono; Sugiyanto Dummy; Lukman Hakim; Nurlaila Dummy; Arief Rahman Hakim; Ika Puspitasari; Arief Nurrochmad; Purwantiningsih Dummy; Oetari Dummy; Tedjo Yuwono
INDONESIAN JOURNAL OF PHARMACY | 2012
Agung Endro Nugroho; Arief Rahman Hakim; Purwantiningsih; Lukman Hakim
Nuclear Engineering and Technology | 2018
Arief Rahman Hakim; Andang Widi Harto; Alexander Agung
World Academy of Science, Engineering and Technology, International Journal of Pharmacological and Pharmaceutical Sciences | 2017
Endang Lukitaningsih; Fathul Jannah; Arief Rahman Hakim; Ratna D. Puspita; Zullies Ikawati
JURNAL BIOLOGI INDONESIA | 2017
Ahmad Fudholi; Edy Meiyanto; Imono Argo Donatus; Arief Nurrochmad; Arief Rahman Hakim; Retno Murwanti
INDONESIAN JOURNAL OF PHARMACY | 2017
Akhmad Kharis Nugroho; Arief Rahman Hakim; Lukman Hakim
INDONESIAN JOURNAL OF PHARMACY | 2016
Pudjono Pudjono; Jessica Anindita; Arief Rahman Hakim; Hari Purnomo