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Dive into the research topics where Daniel J. Dickinson is active.

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Featured researches published by Daniel J. Dickinson.


Nature Methods | 2013

Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination

Daniel J. Dickinson; Jordan D. Ward; David J. Reiner; Bob Goldstein

Study of the nematode Caenorhabditis elegans has provided important insights in a wide range of fields in biology. The ability to precisely modify genomes is critical to fully realize the utility of model organisms. Here we report a method to edit the C. elegans genome using the clustered, regularly interspersed, short palindromic repeats (CRISPR) RNA-guided Cas9 nuclease and homologous recombination. We demonstrate that Cas9 is able to induce DNA double-strand breaks with specificity for targeted sites and that these breaks can be repaired efficiently by homologous recombination. By supplying engineered homologous repair templates, we generated gfp knock-ins and targeted mutations. Together our results outline a flexible methodology to produce essentially any desired modification in the C. elegans genome quickly and at low cost. This technology is an important addition to the array of genetic techniques already available in this experimentally tractable model organism.


Genetics | 2015

Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette

Daniel J. Dickinson; Ariel M. Pani; Jennifer K. Heppert; Christopher D. Higgins; Bob Goldstein

A central goal in the development of genome engineering technology is to reduce the time and labor required to produce custom genome modifications. Here we describe a new selection strategy for producing fluorescent protein (FP) knock-ins using CRISPR/Cas9-triggered homologous recombination. We have tested our approach in Caenorhabditis elegans. This approach has been designed to minimize hands-on labor at each step of the procedure. Central to our strategy is a newly developed self-excising cassette (SEC) for drug selection. SEC consists of three parts: a drug-resistance gene, a visible phenotypic marker, and an inducible Cre recombinase. SEC is flanked by LoxP sites and placed within a synthetic intron of a fluorescent protein tag, resulting in an FP–SEC module that can be inserted into any C. elegans gene. Upon heat shock, SEC excises itself from the genome, leaving no exogenous sequences outside the fluorescent protein tag. With our approach, one can generate knock-in alleles in any genetic background, with no PCR screening required and without the need for a second injection step to remove the selectable marker. Moreover, this strategy makes it possible to produce a fluorescent protein fusion, a transcriptional reporter and a strong loss-of-function allele for any gene of interest in a single injection step.


Genetics | 2016

CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering.

Daniel J. Dickinson; Bob Goldstein

The advent of genome editing techniques based on the clustered regularly interspersed short palindromic repeats (CRISPR)–Cas9 system has revolutionized research in the biological sciences. CRISPR is quickly becoming an indispensible experimental tool for researchers using genetic model organisms, including the nematode Caenorhabditis elegans. Here, we provide an overview of CRISPR-based strategies for genome editing in C. elegans. We focus on practical considerations for successful genome editing, including a discussion of which strategies are best suited to producing different kinds of targeted genome modifications.


PLOS ONE | 2015

Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation.

Hayretin Yumerefendi; Daniel J. Dickinson; Hui Wang; Seth P. Zimmerman; James E. Bear; Bob Goldstein; Klaus M. Hahn; Brian Kuhlman

Light-activatable proteins allow precise spatial and temporal control of biological processes in living cells and animals. Several approaches have been developed for controlling protein localization with light, including the conditional inhibition of a nuclear localization signal (NLS) with the Light Oxygen Voltage (AsLOV2) domain of phototropin 1 from Avena sativa. In the dark, the switch adopts a closed conformation that sterically blocks the NLS motif. Upon activation with blue light the C-terminus of the protein unfolds, freeing the NLS to direct the protein to the nucleus. A previous study showed that this approach can be used to control the localization and activity of proteins in mammalian tissue culture cells. Here, we extend this result by characterizing the binding properties of a LOV/NLS switch and demonstrating that it can be used to control gene transcription in yeast. Additionally, we show that the switch, referred to as LANS (light-activated nuclear shuttle), functions in the C. elegans embryo and allows for control of nuclear localization in individual cells. By inserting LANS into the C. elegans lin-1 locus using Cas9-triggered homologous recombination, we demonstrated control of cell fate via light-dependent manipulation of a native transcription factor. We conclude that LANS can be a valuable experimental method for spatial and temporal control of nuclear localization in vivo.


Molecular Biology of the Cell | 2016

Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system

Jennifer K. Heppert; Daniel J. Dickinson; Ariel M. Pani; Christopher D. Higgins; Annette Steward; Julie Ahringer; Jeffrey R. Kuhn; Bob Goldstein

Fluorescent protein tags are the primary tool for labeling gene products and analyzing their dynamics using live-cell imaging. A quantitative comparison is made of fluorescent protein brightness and photostability in an in vivo animal model system, and tools and recommendations are given for optimal fluorescent protein selection.


Molecular Biology of the Cell | 2015

Crescerin uses a TOG domain array to regulate microtubules in the primary cilium

Alakananda Das; Daniel J. Dickinson; Cameron C. Wood; Bob Goldstein; Kevin C. Slep

Primary cilia are critical organelles involved in development, sensation, and signaling. Crescerin, a conserved protein family in ciliated and flagellated eukaryotes, uses a TOG domain array with tubulin polymerization activity to regulate cilia microtubules and facilitate proper cilia length, ultrastructure, and function.


Genetics | 2016

Identifying Regulators of Morphogenesis Common to Vertebrate Neural Tube Closure and Caenorhabditis elegans Gastrulation.

Jessica Sullivan-Brown; Panna Tandon; Kim E. Bird; Daniel J. Dickinson; Sophia C. Tintori; Jennifer K. Heppert; Joy H. Meserve; Kathryn P. Trogden; Sara K. Orlowski; Frank L. Conlon; Bob Goldstein

Neural tube defects including spina bifida are common and severe congenital disorders. In mice, mutations in more than 200 genes can result in neural tube defects. We hypothesized that this large gene set might include genes whose homologs contribute to morphogenesis in diverse animals. To test this hypothesis, we screened a set of Caenorhabditis elegans homologs for roles in gastrulation, a topologically similar process to vertebrate neural tube closure. Both C. elegans gastrulation and vertebrate neural tube closure involve the internalization of surface cells, requiring tissue-specific gene regulation, actomyosin-driven apical constriction, and establishment and maintenance of adhesions between specific cells. Our screen identified several neural tube defect gene homologs that are required for gastrulation in C. elegans, including the transcription factor sptf-3. Disruption of sptf-3 in C. elegans reduced the expression of early endodermally expressed genes as well as genes expressed in other early cell lineages, establishing sptf-3 as a key contributor to multiple well-studied C. elegans cell fate specification pathways. We also identified members of the actin regulatory WAVE complex (wve-1, gex-2, gex-3, abi-1, and nuo-3a). Disruption of WAVE complex members reduced the narrowing of endodermal cells’ apical surfaces. Although WAVE complex members are expressed broadly in C. elegans, we found that expression of a vertebrate WAVE complex member, nckap1, is enriched in the developing neural tube of Xenopus. We show that nckap1 contributes to neural tube closure in Xenopus. This work identifies in vivo roles for homologs of mammalian neural tube defect genes in two manipulable genetic model systems.


ChemBioChem | 2018

Light‐Dependent Cytoplasmic Recruitment Enhances the Dynamic Range of a Nuclear Import Photoswitch

Hayretin Yumerefendi; Hui Wang; Daniel J. Dickinson; Andrew Michael Lerner; Per Malkus; Bob Goldstein; Klaus M. Hahn; Brian Kuhlman

Cellular signal transduction is often regulated at multiple steps to achieve more complex logic or precise control of a pathway. For instance, some signaling mechanisms couple allosteric activation with localization to achieve high signal to noise. Here, we create a system for light‐activated nuclear import that incorporates two levels of control. It consists of a nuclear import photoswitch, light‐activated nuclear shuttle (LANS), and a protein engineered to preferentially interact with LANS in the dark, Zdk2. First, Zdk2 is tethered to a location in the cytoplasm that sequesters LANS in the dark. Second, LANS incorporates a nuclear localization signal (NLS) that is sterically blocked from binding to the nuclear import machinery in the dark. If activated with light, LANS both dissociates from its tethered location and exposes its NLS, which leads to nuclear accumulation. We demonstrate that this coupled system improves the dynamic range of LANS in mammalian cells, yeast, and Caenorhabditis elegans and provides tighter control of transcription factors that have been fused to LANS.


eLife | 2018

Optogenetic dissection of mitotic spindle positioning in vivo

Lars-Eric Fielmich; Ruben Schmidt; Daniel J. Dickinson; Bob Goldstein; Anna Akhmanova; Sander van den Heuvel

The position of the mitotic spindle determines the plane of cell cleavage, and thereby daughter cell location, size, and content. Spindle positioning is driven by dynein-mediated pulling forces exerted on astral microtubules, which requires an evolutionarily conserved complex of Gα∙GDP, GPR-1/2Pins/LGN, and LIN-5Mud/NuMA proteins. To examine individual functions of the complex components, we developed a genetic strategy for light-controlled localization of endogenous proteins in C. elegans embryos. By replacing Gα and GPR-1/2 with a light-inducible membrane anchor, we demonstrate that Gα∙GDP, Gα∙GTP, and GPR-1/2 are not required for pulling-force generation. In the absence of Gα and GPR-1/2, cortical recruitment of LIN-5, but not dynein itself, induced high pulling forces. The light-controlled localization of LIN-5 overruled normal cell-cycle and polarity regulation and provided experimental control over the spindle and cell-cleavage plane. Our results define Gα∙GDP–GPR-1/2Pins/LGN as a regulatable membrane anchor, and LIN-5Mud/NuMA as a potent activator of dynein-dependent spindle-positioning forces.


bioRxiv | 2018

C. elegans RAP-1 reinforces LET-60/Ras induction of cell fate

Neal R. Rasmussen; Daniel J. Dickinson; David J. Reiner

The notoriety of the small GTPase Ras as the most mutated oncoprotein has led to a well-characterized signaling network largely conserved across metazoans. Yet the role of its close relative Rap1 (Ras Proximal), which shares 100% identity between their core effector binding sequences, remains unclear. A long-standing controversy in the field is whether Rap1 also functions to activate the canonical Ras effector, the S/T kinase Raf. We used the developmentally simpler Caenorhabditis elegans, which lacks the extensive paralog redundancy of vertebrates, to examine the role of RAP-1 in two distinct LET-60/Ras-dependent cell fate patterning events: induction of 1˚ vulval precursor cell (VPC) fate and of the excretory duct cell. Fluorescently tagged endogenous RAP-1 is localized to plasma membranes and is expressed ubiquitously, with even expression levels across the VPCs. RAP-1 and its activating GEF PXF-1 function cell autonomously and are necessary for maximal induction of 1˚ VPCs. Critically, mutationally activated endogenous RAP-1 is sufficient both to induce ectopic 1˚s and duplicate excretory duct cells. Like endogenous RAP-1, before induction GFP expression from the pxf-1 promoter is uniform across VPCs. However, unlike endogenous RAP-1, after induction GFP expression is increased in presumptive 1˚s and decreased in presumptive 2˚s. We conclude that RAP-1 is a positive regulator that promotes Ras-dependent inductive fate decisions. We hypothesize that PXF-1 activation of RAP-1 serves as a minor parallel input into the major LET-60/Ras signal through LIN-45/Raf.

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Bob Goldstein

University of North Carolina at Chapel Hill

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Ariel M. Pani

University of North Carolina at Chapel Hill

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Christopher D. Higgins

University of North Carolina at Chapel Hill

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David J. Reiner

University of North Carolina at Chapel Hill

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Jennifer K. Heppert

University of North Carolina at Chapel Hill

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Brian Kuhlman

University of North Carolina at Chapel Hill

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Hayretin Yumerefendi

University of North Carolina at Chapel Hill

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Hui Wang

University of North Carolina at Chapel Hill

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Klaus M. Hahn

University of North Carolina at Chapel Hill

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