Ella Salamah

EKSTRAKSI DAN KARAKTERISASI PARSIAL EKSTRAK KASAR ENZIM KATEPSIN DARI IKAN PATIN [Extraction and Partial Characterization of Crude Enzymes Cathepsin from Catfish]

EKSTRAKSI DAN KARAKTERISASI PARSIAL EKSTRAK KASAR ENZIM KATEPSIN DARI IKAN PATIN [Extraction and Partial Characterization of Crude Enzymes Cathepsin from Catfish] Muhammad Zakiyul Fikri* , Tati Nurhayati dan Ella Salamah Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Bogor Diterima 02 Juli 2013 / Disetujui 30 Mei 2014 ABSTRACT Decomposition of protein by enzymatic process will lead to changes in odor, texture, and appearance of fish. The enzymes that play a role in the enzymatic process is primarily proteolytic enzymes. Cathepsin is one of the proteolytic enzymes found in animal tissue that hydrolyzes peptide bonds of proteins. This study aims to extract the cathepsin, characterize the crude extract derived from catfish. The stages of this research consist of the extraction and characterization of the cathepsin from catfish . Result of the extraction was crude extract of c athepsin with activity of 0.278 U/mL. The enzyme had optimum temperature of 50°C, pH 6 and substrate concentration of 2%. The activity of the cathepsin was inhibited by metal ions of Fe 3+ , Cu 2+ , Ca 2+ , but increased by metal ions of Mg 2+ .

Extraction and partial characterization of crude enzymes cathepsin from catfish.

EKSTRAKSI DAN KARAKTERISASI PARSIAL EKSTRAK KASAR ENZIM KATEPSIN DARI IKAN PATIN [Extraction and Partial Characterization of Crude Enzymes Cathepsin from Catfish] Muhammad Zakiyul Fikri* , Tati Nurhayati dan Ella Salamah Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Bogor Diterima 02 Juli 2013 / Disetujui 30 Mei 2014 ABSTRACT Decomposition of protein by enzymatic process will lead to changes in odor, texture, and appearance of fish. The enzymes that play a role in the enzymatic process is primarily proteolytic enzymes. Cathepsin is one of the proteolytic enzymes found in animal tissue that hydrolyzes peptide bonds of proteins. This study aims to extract the cathepsin, characterize the crude extract derived from catfish. The stages of this research consist of the extraction and characterization of the cathepsin from catfish . Result of the extraction was crude extract of c athepsin with activity of 0.278 U/mL. The enzyme had optimum temperature of 50°C, pH 6 and substrate concentration of 2%. The activity of the cathepsin was inhibited by metal ions of Fe 3+ , Cu 2+ , Ca 2+ , but increased by metal ions of Mg 2+ .

EKSTRAKSI DAN KARAKTERISASI PARSIAL EKSTRAK KASAR ENZIM KATEPSIN DARI IKAN PATIN

EKSTRAKSI DAN KARAKTERISASI PARSIAL EKSTRAK KASAR ENZIM KATEPSIN DARI IKAN PATIN [Extraction and Partial Characterization of Crude Enzymes Cathepsin from Catfish] Muhammad Zakiyul Fikri* , Tati Nurhayati dan Ella Salamah Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Bogor Diterima 02 Juli 2013 / Disetujui 30 Mei 2014 ABSTRACT Decomposition of protein by enzymatic process will lead to changes in odor, texture, and appearance of fish. The enzymes that play a role in the enzymatic process is primarily proteolytic enzymes. Cathepsin is one of the proteolytic enzymes found in animal tissue that hydrolyzes peptide bonds of proteins. This study aims to extract the cathepsin, characterize the crude extract derived from catfish. The stages of this research consist of the extraction and characterization of the cathepsin from catfish . Result of the extraction was crude extract of c athepsin with activity of 0.278 U/mL. The enzyme had optimum temperature of 50°C, pH 6 and substrate concentration of 2%. The activity of the cathepsin was inhibited by metal ions of Fe 3+ , Cu 2+ , Ca 2+ , but increased by metal ions of Mg 2+ .

Chrome Tanning Leather of Giant Sea Perch Combined with Seed Extract Areca Nut on the Physical Characteristics

Tanning is the process of converting raw hide protein to leather, which are stable, not easily decompose, and is suitable for a variety of uses. The use of vegetable based tanning materials in the leather tanning process has not been carried out. Vegetable based materials that were used are betel nuts. This plant contains tannin which is the main agent in the process of leather tanning. The aim of this study was to determine the physical characteristics of snapper leather treated with betel nut extract. Soxhlet extracting method with methanol as a solvent were used to obtain tannin from betel nuts. Tanned Snapper Leather were analyzed for physical quality, elongation strength, tensile strength, tear strength, and sewing strength. The result showed that methanol extracted betel nut with 10% concentration gives the optimum physical characteristics.