Faruk Turgay

Effects of Anaerobic Training on Paraoxonase-1 Enzyme (PON1) Activities of High Density Lipoprotein Subgroups and Its Relationship with PON1-Q192R Phenotype

AIM Paraoxonase-1 (PON1) is an antiatherosclerotic enzyme located on high-density lipoprotein (HDL). The effects of anaerobic exercise on PON1 activity are unknown. Here we investigated the effects of anaerobic judo training on three different activities of same PON1 enzyme (TDPON1), including basal PON1, salt-stimulated PON1 (SPON1), and arylesterase (AE) activities, of serum, HDL, and HDL subgroups (HDLs; HDL and its subgroups) and its relationship with PON1-Q192R phenotype (PON1P). METHODS Our study included 18 Turkish national female judoists (mean age: 17.9 ± 0.8 years). Before and after 5 months of anaerobic training, critical speed (CS), TDPON1 activities, cholesterol levels in the serum and supernatants of HDLs obtained by polyethylene glycol, and other major blood lipids and lipoproteins (BLLPs) including triglycerides were determined using blood samples taken after overnight fasting. PON1P groups (PGs) were categorized as QQ (QG; persons with low activity) and R carriers (QR + RR) (RG; persons with high activity) according to SPON1/AE activity ratios. The results were considered statistically significant at P ≤ 0.05. RESULTS Anaerobic training resulted in significantly increased the cholesterol levels of HDLs (except HDL2-C) in all subjects, but not HDLs-C in PGs. Anaerobic training resulted in significant increases in most TDPON1 activities of serum and HDLs in all subjects and (except AE) in PGs, whereas SPON1 and HDL2 AE activities increased only in the RG, which was related to PON1P. However, PON1P was not related to other measured markers, including basal BLLP profiles. CONCLUSIONS Anaerobic training improved most TDPON1 activities of serum and HDLs and HDLs -C levels (except HDL2-C) in all subjects, but not HDLs-C in PGs. The beneficial effects of anaerobic training on SPON1 and HDL2 AE activities were depend on PON1P. The lack of response of HDL2-C to anaerobic exercise will require further research.

Effects of aerobic training on serum paraoxonase activity and its relationship with PON1-192 phenotypes in women

Background Paraoxonase 1 (PON1) is an antioxidant enzyme that protects high-density lipoprotein (HDL) and low-density lipoprotein against oxidation. Limited studies have addressed the influence of exercise on PON1 activity and its relationship with PON1 phenotypes. We investigated relationships between PON1-192 phenotypes, PON1 activity, aerobic exercise, and blood lipid and lipoprotein concentrations in middle-aged women. Methods An exercise group (n = 50) engaging in regular aerobic exercise and a control group (n = 41) were selected from a subset of 300 Caucasian women that met the inclusion criteria. Serum PON1, salt-stimulated PON1 (SSPON1), and arylesterase (ARE) activities; cholesterol levels and ARE activities of total HDL and HDL subgroups (HDLs) (supernatants obtained by polyethylene glycol); and blood lipid and lipoprotein concentrations were determined by standardized enzymatic methods. PON1-192 QQ (low activity), QR (moderate activity), and RR (high activity) phenotype groups were defined using serum SSPON1/ARE activity ratios. The R-carries (RC) phenotype group consisted of the QR and RR groups combined. Results All lipid and lipoprotein concentrations were greater in the exercise group than in the control group. Regardless of phenotype, no significant differences were observed between the exercise and control groups in terms of serum PON1, SSPON1, or ARE activity associated with HDLs (p > 0.05), whereas PON1 activities in QQ-phenotyped women in the exercise group were significantly higher than those in the control group (p < 0.01), but not the RC group. A statistically significant interaction between PON1 phenotypes (QQ and RC groups) and exercise (exercise and control groups) on PON1 activity was found. Conclusion These results showed that a regular aerobic exercise program can improve PON1 activity depending on PON1-192 phenotype, but not on lipid and lipoprotein levels, in middle-aged Turkish women.

The Physiological Responses to 5000 m Open Water Swimming Exercise in Children

Objective: Open water swimming (OWS) is an endurance sport. Nitric oxide (NO) is a vasodilator gas, therefore it is related to endurance. Physiological stress levels of OWS on children are unknown. The purposes of this study were to investigate the physiological responses to 5000 m OWS and its relation with serum NOx (nitrite plus nitrate) levels in children. Material and Methods: Trained OWS athletes (9 males and 5 females; age=13.8±1.12 years) participated in the study. Serum NOx, oxidative stress index (OSI): total antioxidant status (TAS)/total oxidant stress status (TOS) ratio, muscle damage markers (MDM): serum creatine kinase (CK), aspartate aminotransferase (AST) activity, leucocyte counts and serum glucose (GLU) levels were determined before and after the OWS. Lactate (La), whole blood glucose (WGLU) analyses were done from the fingertip blood samples and heart rate (HR) was measured after every 1000 m. Results: After 5000 m OWS, HR and La levels were recorded as 166 beat/min and 2.95 mM. The results indicated that MDM significantly increased (p<0.01), and OSI significantly decreased however there were no significantly changes in TAS, NOx and serum glucose (GLU) values. Basal NOx levels was only related to CK (r=-0.529, p=0.052), but not other parameters. Conclusion: 5000 m OWS exercise induced a moderate physiological stress without any oxidative stress, hypoglycemia and health risk for the investigated parameters risk. Although this exercise did not increase blood NOx levels, the negative relationship between basal NOx and CK parameters may show the anti-inflammatory role of NO during OWS exercises in the children.

Effects of ribose supplementation on anaerobic performance, plasma pH, lactate, ammonia and inorganic phosphate levels

The purpose of this study was to estimate the effects of ribose supplementation on interval anaerobic performance and fatiguing substance concentrations. The study cohort consisted of physically active, young male volunteers (n = 15). With a double-blind cross over study design, each participant completed two all-out anaerobic exercise sessions, one week apart, one by supplementing maltodextrine and the other by supplementing ribose (150 mg/kg in each test). Two repetitions of 30-s maximal performance with a 4-min interval were completed. Blood samples were taken at rest and five minutes after the second sprint repetition. Neither the biochemical parameters: pH (−0.32± 0.09 vs.−0.26± 0.11), lactate (La) (13.8± 1.9 vs. 13.3± 2.7 mM, ammonia (423 ± 190 vs. 404 ± 166 μmol/L), inorganic phosphate (Pi) (0.68 ± 0.53 vs. 0.72 ± 0.63 mM) nor the anaerobic output (746.2 ± 82.5 vs. 760.2 ± 86.8 watts) were affected by placebo and ribose supplementations significantly (p > 0.05). In conclusion, based on this protocol, anaerobic performance and/or biochemical status are not affected by ribose supplementation.