Francisco Cláudio Dantas Mota

Cryptococcus neoformans var. grubii-Induced Arthritis with Encephalitic Dissemination in a Dog and Review of Published Literature.

This article describes the clinical, pathological, and immunohistochemical findings associated with Cryptococcus neoformans var. grubii in a 4-year-old female Boxer dog from Uberlândia, Minas Gerais, Southeastern Brazil. Clinically, there was a swelling at the right metatarsal region and the hock joint with enlargement of regional lymph nodes. Radiographical evaluation revealed lysis of the tarsal bone; cytology demonstrated cryptococcal intralesional organisms at the swollen joint. Despite empirical antifungals therapeutic, the animal developed neurological cryptococcosis and died spontaneously. Significant pathological alterations included arthritis, lymphadenitis, and encephalitic cryptococcomas associated with numerous intralesional narrow-necked budding encapsulated yeasts. Immunohistochemistry utilising monoclonal antibodies that label C. neoformans sp. complex capsule, characterised the yeasts as C. neoformans var. grubii. Collectively, the pathological and immunohistochemical findings of this dog indicate that the intralesional organisms observed within the articular surface of the hock joint, lymph nodes, and the brain were C. neoformans var. grubii, confirming the participation of this fungal pathogen in the development of cryptococcal arthritis. In this case, the most likely pathogenesis was percutaneous inoculation with resultant abscess-like lesion, which resulted in the draining sinus, swelling of the right hind limb with progression to the articular disease. Thereafter, the fungal pathogen probably compromised the adjacent lymph nodes with subsequent haematogenous distribution to the brain, terminating with cryptococcal arthritis, lymphadenitis, and encephalitis.

Análise ultra-estrutural da túnica muscular do intestino delgado de cães preservado em diferentes meios

The present study evaluated twenty-five sarnples of the muscular layer of the small gut in dogs, which were preserved in a 300% sugar supersaturated. solution, 98% glycerin, 5% polyvinil-pirrolidona and tiomersal tincture at 1: 1000. The samples were maintained under conservation for 45 days and further submitted to ultrastructural assay by transmission electron microscopy. The cellular integrity was not entirely preserved when using the media, while the 300% sugar solution and 98% glycerin were the preservation conditions that better maintained the cellular ultrastructure.

Utilização do adesivo N-butil cianoacrilato e o do fio poliglactina na rafia de córnea em coelhos (Oryctolagus cunicullus)

The aim of this study was to evaluate the cicatricial repair of perforated cornea in rabbits, by using the N-butyl cyanoacrylate adhesive compared to the 910-polyglactine no 7-0 thread suture through macroscopic and histological assays. Corneas from 18 adult rabbits were perforated and soon after submitted the synthesis with N-butyl cyanoacrylate synthetic adhesive right ocular globe or by simple interrupted sutures using the 910-polyglactine thread left ocular globe. The animals were separate in three groups of the same number. Examination after 7, 15, and 30 days post-operative showed that both the synthetic adhesive and the suture were efficient in the synthesis of the surgical wounds, thus stabilizing the intra-ocular contents. The N-butyl cyanoacrylate adhesive was showed to be superior to the 910-polyglactine suture thread with regards to the evolution and the organization of the healing process.

Comportamento das artérias extramurais do útero de cães sem raça definida (Canis familiaris - Linnaeus, 1758)

The present study involved thirty femeale adult dogs coming from the kennel of the Veterinary Hospital of the Federal University of Uberlândia, Minas Gerais, Brazil. The thoracic cavity of the animal was opened and the aorta astery (thoracic part) was locared and canulated. A 450 Neoprene latex solution was used to injet the aorta astery in caudal direction. The specimens were then fixed in a 10 % formol aqueous solution. The results showed that the ovaric, uterine and vaginal arteries supply the uterus as follows : (1) the ovaric artery, in all the cases, arose from the aorta artery, either cranially to the caudal mesenteric artery (98,33%) or at same level of this artery (1,67%). In 83,33% of the cases, it gave off a branch to the uterine corns, which was distributed on the dorsal (8,00%), on the ventral (12,00%) and ond the both (80,00%) surfaces. In addition, two uterine branches distributed on the ventral (66,67%) and the dorsal (33,33%) surfaces were found in 10,00% of the cases; (2) the uterine artery, in all the cases, arose from the vaginal artery. In all the specimens the artery uterine supplied the uterine corns, with the ventral surfaces receiving greater mean number of branches than the dorsal surface. Concerning the uterine body the mean number of branches from the uterine artery showed to be greater on the dorsal surface. In 70, 00% of the cases, the uterine cervix received branches from directly from the uterine artery, with greater number of these arterial vessels being found on the ventral surface.

Reparo de esôfago cervical de cães com segmento intestinal livre autólogo desprovido de epitélio e de lâmina própria da túnica mucosa

Twelve dogs were allocated in two groups with same number of animals and submitted to removal of a 3.0 x 3.5cm piece of the cervical esophagus. The incision was occluded with a autograft of free intestinal segment without epithelium and lamina propria of the mucosa, with separate simples points using poliglactina 910 line. Animals of the group 1 were observed for 15 days post-surgery, and the animals from group 2, for 30 days. Epithelization and discreet stenosis in the place of the graft with invasion of dense conjunctive tissue, rich in collagen fibers was noticed. The autograft free intestinal segment was efficient in the repair of ruptures in the cervical segment of the canine esophagous.

Enterorrafias em coelhos com sutura invertida extramucosa versus continua simples contaminante e cushing: avaliação macroscópica

The aim of this study was to evaluate and compare macroscopically suture patterns with catgut thread on intestinal suture used in rabbits. Ten adult, male, New Zealand rabbits were used. In each animal two longitudinal incisions of 3,0cm were made in small intestine, equidistant one from the other 10,0cm. The cranial incision was repaired with extramucous Swift-like suture and the caudal incision was repaired with simple continuous pattern including mucosa and the second with Cushing, both protected by omentum. After ten days postoperative was observed in Swift-like suture lower stenosis and advanced cicatricial process. It was therefore concluded that the extramucous Swift-like suture can be safety recommended in intestinal surgeries.

Aspectos da irrigação do nó atrioventricular e tronco do fascículo atrioventricular em bovinos mestiços Girolando

Estudaram-se aspectos da irrigacao do no atrioventricular e tronco do fasciculo atrioventricular, componentes do sistema de conducao do coracao. Para tanto, utilizaram-se trinta coracoes de bovinos femeas, mesticas Girolando, com idade entre 36 e 48 meses. Os mesmos foram dissecados, apos previa injecao das arterias coronarias direita e esquerda, com solucao corada de Neoprene latex 450, seguida pela fixacao dos coracoes em solucao aquosa de formol a 15,00%, pelo metodo de imersao. O no atrioventricular foi irrigado, isoladamente ou em associacao, pelo primeiro ramo septal do ramo interventricular paraconal (3,33%), ramo distal do atrio esquerdo (6,66%), ramo septal da arteria coronaria direita (46,66%), ramo proximal do atrio direito (76,66%) e ramo ventricular direito (76,66%). O tronco do fasciculo atrioventricular foi irrigado, isoladamente ou em associacao, pelo primeiro ramo septal do ramo interventricular paraconal (3,33%), ramo ventricular direito (60,00%), ramo proximal do atrio direito (60,00%), e com maior frequencia pelo ramo septal da arteria coronaria direita (70,00%). As anastomoses arteriais estiveram presentes em 76,66% dos casos e geralmente formaram circuitos junto as margens do sistema de conducao cardiaco.