Patrícia Maria Coletto Freitas

Análise ultra-estrutural da túnica muscular do intestino delgado de cães preservado em diferentes meios

The present study evaluated twenty-five sarnples of the muscular layer of the small gut in dogs, which were preserved in a 300% sugar supersaturated. solution, 98% glycerin, 5% polyvinil-pirrolidona and tiomersal tincture at 1: 1000. The samples were maintained under conservation for 45 days and further submitted to ultrastructural assay by transmission electron microscopy. The cellular integrity was not entirely preserved when using the media, while the 300% sugar solution and 98% glycerin were the preservation conditions that better maintained the cellular ultrastructure.

Utilização do adesivo N-butil cianoacrilato e o do fio poliglactina na rafia de córnea em coelhos (Oryctolagus cunicullus)

The aim of this study was to evaluate the cicatricial repair of perforated cornea in rabbits, by using the N-butyl cyanoacrylate adhesive compared to the 910-polyglactine no 7-0 thread suture through macroscopic and histological assays. Corneas from 18 adult rabbits were perforated and soon after submitted the synthesis with N-butyl cyanoacrylate synthetic adhesive right ocular globe or by simple interrupted sutures using the 910-polyglactine thread left ocular globe. The animals were separate in three groups of the same number. Examination after 7, 15, and 30 days post-operative showed that both the synthetic adhesive and the suture were efficient in the synthesis of the surgical wounds, thus stabilizing the intra-ocular contents. The N-butyl cyanoacrylate adhesive was showed to be superior to the 910-polyglactine suture thread with regards to the evolution and the organization of the healing process.

Comportamento das artérias extramurais do útero de cães sem raça definida (Canis familiaris - Linnaeus, 1758)

The present study involved thirty femeale adult dogs coming from the kennel of the Veterinary Hospital of the Federal University of Uberlândia, Minas Gerais, Brazil. The thoracic cavity of the animal was opened and the aorta astery (thoracic part) was locared and canulated. A 450 Neoprene latex solution was used to injet the aorta astery in caudal direction. The specimens were then fixed in a 10 % formol aqueous solution. The results showed that the ovaric, uterine and vaginal arteries supply the uterus as follows : (1) the ovaric artery, in all the cases, arose from the aorta artery, either cranially to the caudal mesenteric artery (98,33%) or at same level of this artery (1,67%). In 83,33% of the cases, it gave off a branch to the uterine corns, which was distributed on the dorsal (8,00%), on the ventral (12,00%) and ond the both (80,00%) surfaces. In addition, two uterine branches distributed on the ventral (66,67%) and the dorsal (33,33%) surfaces were found in 10,00% of the cases; (2) the uterine artery, in all the cases, arose from the vaginal artery. In all the specimens the artery uterine supplied the uterine corns, with the ventral surfaces receiving greater mean number of branches than the dorsal surface. Concerning the uterine body the mean number of branches from the uterine artery showed to be greater on the dorsal surface. In 70, 00% of the cases, the uterine cervix received branches from directly from the uterine artery, with greater number of these arterial vessels being found on the ventral surface.

Reparo de esôfago cervical de cães com segmento intestinal livre autólogo desprovido de epitélio e de lâmina própria da túnica mucosa

Twelve dogs were allocated in two groups with same number of animals and submitted to removal of a 3.0 x 3.5cm piece of the cervical esophagus. The incision was occluded with a autograft of free intestinal segment without epithelium and lamina propria of the mucosa, with separate simples points using poliglactina 910 line. Animals of the group 1 were observed for 15 days post-surgery, and the animals from group 2, for 30 days. Epithelization and discreet stenosis in the place of the graft with invasion of dense conjunctive tissue, rich in collagen fibers was noticed. The autograft free intestinal segment was efficient in the repair of ruptures in the cervical segment of the canine esophagous.

Ruptura de ducto torácico idiopático em um gato: Relato de caso

The idiopathic thoracic duct rupture is rare accurrence in veterinary medicine. This report describes the female feline admission, 5 years old, in the emergency room and intensive care of the Veterinary Hospital of the Federal University of Minas Gerais studying with dyspnea, chest muffled sounds, and large volume of chylous aspect of liquid after thoracic puncture. The rapid collection of pleural fluid after puncture, along with the laboratory aspect of effusion, suspected to break the idiopathic thoracic duct, considering that the patient had no history of trauma, nor access to the street. After thoracotomy, there was a complete rupture of the thoracic duct, the height of the ninth thoracic vertebra, on which a ligature was performed. The patient developed satisfactorily, being discharged ten days after the surgical procedure. We conclude that the rupture of the thoracic duct has a major change in the patients physiology, requiring preparation of the veterinarian because it is an emergency situation by major systemic changes that cause.

Partial ablation of endometrial glands in dogs after exposure to progestin during the neonatal period

Bitches with uteri devoid of endometrial glands should be sterile, and consequently could contribute to the population control of dogs. Considering that an inadequate exposure of the female reproductive system to steroids can lead to the formation of the uterine gland knock-out (UGKO) phenotype in some species, the aim of this study was to evaluate the effect of serial applications of medroxyprogesterone acetate (MPA) from birth until the age of six months on the development of endometrial glands in bitches. For this purpose, 16 female mongrel dogs from different litters were distributed into either an MPA group (n = 8), animals treated with 10 mg kg sc (Promone-E®, Pfizer, Brasil) at 3-week intervals, from day one after birth until the age of six months, or a control group (n = 8), composed of animals that only received a 0.9% NaCl solution in place of MPA. At six months of age, ovariohysterectomy was performed and uterine horn samples were collected for histological and immunohistochemical examinations. The bitches from the MPA-treated group presented a 35% decrease in the number of endometrial glands, a larger diameter of the endometrial glands, a greater epithelial height, as well as a greater thickness of the uterine wall, endometrium, and myometrium. However, no significant differences were observed between the two groups in the expression of ER-α, ER-β, and PR on the surface epithelium and endometrial stroma. Therefore, the serial application of MPA from birth until the age of 6 months do not completely ablate the development of the endometrial glands in bitches, but impair it by 35%.

Enterorrafias em coelhos com sutura invertida extramucosa versus continua simples contaminante e cushing: avaliação macroscópica

The aim of this study was to evaluate and compare macroscopically suture patterns with catgut thread on intestinal suture used in rabbits. Ten adult, male, New Zealand rabbits were used. In each animal two longitudinal incisions of 3,0cm were made in small intestine, equidistant one from the other 10,0cm. The cranial incision was repaired with extramucous Swift-like suture and the caudal incision was repaired with simple continuous pattern including mucosa and the second with Cushing, both protected by omentum. After ten days postoperative was observed in Swift-like suture lower stenosis and advanced cicatricial process. It was therefore concluded that the extramucous Swift-like suture can be safety recommended in intestinal surgeries.