Gumersindo Feijoo

Sodium inhibition in the anaerobic digestion process: Antagonism and adaptation phenomena

Abstract The effect of sodium on the methanization of volatile fatty acid (VFA) mixtures was evaluated for three different sludges. Sodium concentrations causing 50% inhibition ranged from 3 to 16 g l −1 in the absence of nutrients or other salts, showing a higher tolerance to sodium in the sludges obtained from the digesters treating high saline wastewaters. This fact is considered to be a consequence of sludge adaptation to sodium. Furthermore, the adaptation of methanogenic bacteria to sodium was also found during the batch assays: After 40 days of digestion, two different sludges (subjected to 6.9 and 21.5 g Na + l −1 , respectively) increased the relative methanogenic activity from 0% to about 45% of the blank activity. The antagonism phenomena, due to the presence of cations and anions contained in the assayed media, greatly influenced the sodium effect on anaerobic sludges. Sodium concentrations causing 50% inhibition may be increased by 4 to 10 g l −1 or more when sea water is used instead of NaCl. Nutrients also influence the sodium toxicity. The effect of sodium in each step of the anaerobic digestion process appears to be different depending on the sludge. For an adapted sludge, sodium caused 50% inhibition of propionic, acetic and n -butyric utilizers at concentrations of 10.5, 17, and 19 g l −1 , respectively, an indication that propionic utilizers had less adaptation potential. These concentrations ranged between 5 and 6 g l −1 for an unadapted sludge. The methanization of a more complex substance, such as glucose, was less extensively affected than the methanization of VFA. Results from continuous assays agree with those obtained from batch assays.

Simplified life cycle assessment of galician milk production

Milk, an example of staple food, has an outstanding demand by consumers as it is considered a prescription for good health. Life cycle assessment (LCA) is an excellent tool of environmental management and it provides a widespread knowledge on the environmental burdens associated to a product or to a human activity. In this study, a simplified methodology of LCA has been applied to analyse a representative scheme of milk production in Galicia (Spain), where a reliable inventory is still lacking. High quality data for the inventory was obtained in different representative fodder factories, farms and dairies for time periods over two years. The analysis of these data by LCA has permitted to quantify the potential impact associated to milk production and also to determine the reductions attained by the application of different improvement actions, such as the most adequate formulation of cattle feed and the implementation of treatment systems for water and air emissions. The consideration of these actions can lead to a maximum reduction of almost 22% of the global normalised impact.

Environmental and economic profile of six typologies of wastewater treatment plants.

The objective of wastewater treatment plants (WWTPs) is to prevent pollution. However, it is necessary to assess their sustainability in order to ensure that pollution is being removed, not displaced. In this research, the performance of 24 WWTPs has been evaluated using a streamlined Life Cycle Assessment (LCA) with Eutrophication Potential (EP) and Global Warming Potential (GWP) as environmental indicators, and operational costs as economic indicators. WWTPs were further classified in six typologies by their quality requirements according to their final discharge point or water reuse. Moreover, two different functional units (FU), one based on volume (m(3)) and the other on eutrophication reduction (kg PO(4)(3-) removed) were used to further determine sustainability. A correlation between legal requirements and technologies used to achieve them was found: Organic matter removal plants were found to be less costly both in environmental and economic terms if volume was used as the functional unit, while more demanding typologies such as reuse plants showed a trade-off between lower EP and higher cost and GWP; however, this is overcome if the second FU is used instead, proving the sustainability of these options and that this FU better reflects the objectives of a WWTP.

Benchmarking environmental and operational parameters through eco-efficiency criteria for dairy farms

Life Cycle Assessment (LCA) is often used for the environmental evaluation of agri-food systems due to its holistic perspective. In particular, the assessment of milk production at farm level requires the evaluation of multiple dairy farms to guarantee the representativeness of the study when a regional perspective is adopted. This article shows the joint implementation of LCA and Data Envelopment Analysis (DEA) in order to avoid the formulation of an average farm, therefore preventing standard deviations associated with the use of average inventory data while attaining the characterization and benchmarking of the operational and environmental performance of dairy farms. Within this framework, 72 farms located in Galicia (NW Spain) were subject to an LCA+DEA study which led to identify those farms with an efficient operation. Furthermore, target input consumption levels were benchmarked for each inefficient farm, and the corresponding target environmental impacts were calculated so that eco-efficiency criteria were verified. Thus, average reductions of up to 38% were found for input consumption levels, leading to impact reductions above 20% for every environmental impact category. Finally, the economic savings arising from efficient farming practices were also estimated. Economic savings of up to 0.13€ per liter of raw milk were calculated, which means extra profits of up to 40% of the final raw milk price.

The link between operational efficiency and environmental impacts: A joint application of Life Cycle Assessment and Data Envelopment Analysis

Life Cycle Assessment (LCA) allows the estimation of the environmental impacts of a process or product. Those environmental impacts depend on the efficiency with which operations are carried out. In the case that LCA data are available for multiple similar installations, their respective operational performances can be benchmarked and links between operational efficiency and environmental impacts can be established. In this paper, this possibility is illustrated with a case study on LCA of mussel cultivation in rafts. For each site (raft) both its inputs consumption and mussel production are known. A separate LCA of each site has been performed and its corresponding environmental impacts have been estimated. Using Data Envelopment Analysis (DEA) on the input/output data allows computing the relative efficiency of each mussel raft and setting appropriate efficiency targets. The DEA targets represent virtual cultivation sites, which consume less input and/or produce more output. The performance of an LCA study for each of these virtual cultivation sites and the comparison between their environmental impacts are used to estimate the environmental impacts consequences of operational inefficiencies. This direct link can help to convince the managers and operators of the cultivation sites of the double dividend of reducing inputs consumption and achieve operational efficiency: lower costs and lower environmental impacts.

Evaluation of different fungal strains in the decolourisation of synthetic dyes

Of seven fungal strains tested for their ability to decolourise three structurally diverse synthetic dyes, Phanerochaete sordida, Bjerkandera sp. BOS55, Phlebia radiata, and Phanerochaete chrysosporium had average values of maximum decolourisation rates higher than 0.2 Δ[Absorbance] d−1. All seven fungi produced manganese peroxidase (MnP) but laccase activity was detected only in Phlebia radiata. No lignin peroxidase (LiP) activity was observed.

Biobleaching of oxygen delignified kraft pulp by several white rot fungal strains

Twenty-five white rot fungal strains were tested for their ability to bleach Eucalyptus globulus oxygen delignified kraft pulp (OKP). Under nitrogen-limited culture conditions, eight outstanding biobleaching strains were identified that increased the brightness of OKP by more than 10 ISO units compared to pulp incubated in sterile control medium. The highest brightness gain of approximately 13 ISO units was obtained with Bjerkandera sp. strain BOS55, providing a high final brightness of 82% ISO. This strain also caused the greatest level of delignification, decreasing the kappa number of OKP by 29%. When the white rot fungal strains were tested in nitrogen-sufficient medium, the extracellular activities of laccase and peroxidases increased in many strains; nonetheless, the pulp handsheets were either destroyed or brightness gains were lower than those obtained under nitrogen-limitation. The titer of ligninolytic enzymes was not found to be indicative of biobleaching potential. However, the best biobleaching strains were generally characterized by a predominance of manganese dependent peroxidase (MnP) activity compared to other ligninolytic enzymes and by a high decolorizing activity towards the polyanthraquinone ligninolytic indicator dye, Poly R-478.

Environmental assessment of anaerobically digested sludge reuse in agriculture: potential impacts of emerging micropollutants.

Agricultural application of sewage sludge has been emotionally discussed in the last decades, because the latter contains organic micropollutants with unknown fate and risk potential. In this work, the reuse of anaerobically digested sludge in agriculture is evaluated from an environmental point of view by using Life Cycle Assessment methodology. More specifically, the potential impacts of emerging micropollutants, such as pharmaceuticals and personal care products, present in the sludge have been quantified. Four scenarios were considered according to the temperature of the anaerobic digestion (mesophilic or thermophilic) and the sludge retention time (20 or 10d), and they have been compared with the non-treated sludge. From an environmental point of view, the disposal of undigested sludge is not the most suitable alternative, except for global warming due to the dominance (65-85%) of the indirect emissions associated to the electricity use. Nutrient-related direct emissions dominate the eutrophication category impact in all the scenarios (>71.4%), although a beneficial impact related to the avoidance of industrial fertilisers production is also quantified (up to 6.7%). In terms of human and terrestrial toxicity, the direct emissions of heavy metals to soil dominate these two impact categories (>70%), and the contribution of other micropollutants is minimal. Moreover, only six (Galaxolide, Tonalide, Diazepam, Ibuprofen, Sulfamethoxazole and 17alpha-ethinyloestradiol) out of the 13 substances considered are really significant since they account for more than 95% of the overall micropollutants impact.

Environmental Performance of a Municipal Wastewater Treatment Plant

Goal, Scope and BackgroundNowadays, every strategy must be developed taking into account the global impact on the environment; if this aspect is forgotten, a change of environmental loads or their effect will be caused and no reduction will be attained. For instance, a wastewater treatment plant (WWIP), which is considereda priori as an ecological treatment system, gives rise to an environmental impact due to its energy consumption, use of chemical compounds, emissions to the atmosphere and sludge production, the post-treatment of which will also have diverse environmental effects. The goal of this study is to evaluate the potential environmental impact corresponding to a municipal WW1P and to identify the hot spots associated with the process.MethodsIn this study, the Centre of Environmental Science (CML) of Leiden University methodology has been considered to quantify the potential environmental impact associated with the system under study. A comprehensive analysis of the WWTP was evaluated for the physico-chemical characterisation of the wastewaters as well as the inventory of all the inputs (energy, chemical compounds, ...) and outputs (emissions to air, water, soil and solid waste generation) associated with the global process. Regarding Life Cycle Inventory Assessment, SimaPro 5.0 was used and in particular CML factors (updated in 2002) were chosen for characterisation and normalisation stages.Results and DiscussionA comprehensive inventory of empirical data from water, sludge and gas flows during 2000 and 2001 was obtained. Two impact categories arise due to their significance: eutrophication and terrestrial ecotoxicity. Consequently, the aspects to be minimised in order to reduce the environmental impact of the system are the pollutant load at the watercourse discharge (mainly NH3, PO4[3- and COD, even when all of them are below legal limits) and the emissions to soil (mainly Cr, Hg and Zn, even when they are present in low concentrations) when the sludge is used for agricultural application.ConclusionsAs far as the environmental impact is concerned, differentiation between humid and dry season is not required as results are practically equal for both situations. Water discharge and sludge application to land have turned out to be the main contributors in the environmental performance of a WWTP. Regarding the former, the removal of nitrogen by means of a nitrification-denitrification system coupled to conventional biological aerobic treatment implies a high environmental impact reduction and, as for the latter, bearing in mind the proposed legislation, heavy metals as well as pathogens are supposed to be the key parameters to define the most adequate treatment strategies for the generated sludge.Recommendations and OutlookThis study can serve as a basis for future studies that can apply a similar policy to a great number of wastewater facilities. Besides, features such as different treatment systems and capacities can provide additional information with the final aim of including the environmental vector in the decision-making process when the operation of a WWTP is intended to be optimised. Moreover, sludge must also be a focus of attention due to the expected increase and its major contribution to the global environmental impact of a WWTP, which can determine other treatment alternatives.

Oxidative Degradation of Azo Dyes by Manganese Peroxidase under Optimized Conditions

The application of enzyme‐based systems in waste treatment is unusual, given that many drawbacks are derived from their use, including low efficiency, high costs and easy deactivation of the enzyme. The goal of this study is the development of a degradation system based on the use of the ligninolytic enzyme manganese peroxidase (MnP) for the degradation of azo dyes. The experimental work also includes the optimization of the process, with the objective of determining the influence of specific physicochemical factors, such as organic acids, H2O2 addition, Mn2+ concentration, pH, temperature, enzyme activity and dye concentration. A nearly total decolorization was possible at very low reaction times (10 min) and at high dye concentration (up to 1500 mg L−1). A specific oxidation capacity as high as 10 mg dye degraded per unit of MnP consumed was attained for a decolorization higher than 90%. Among all, the main factor affecting process efficiency was the strategy of H2O2 addition. The continuous addition at a controlled flow permitted the progressive participation of H2O2 in the catalytic cycle through a suitable regeneration of the oxidized form of the enzyme, which enhanced both the extent and the rate of decolorization. It was also found that, in this particular case, the presence of a chelating organic acid (e.g., malonic) was not required for an effective operation. Probably, Mn3+ was chelated by the dye itself. The simplicity and high efficiency of the process open an interesting possibility of using of MnP for solving other environmental problems.