Hiromi Okabe

FeLV-related sequences in DNA from a FeLV-free cat colony

Abstract A tritiated DNA transcript of the Gardner-Arnstein strain of feline sarcoma virus was utilized in hybridization assays with cat tissues from a specific pathogen-free cat colony. This colony has been free of feline type-C viruses (FeLV) and FeLV-related disease. The SPF tissue DNA contained FeLV-related sequences to the same extent as tissues from conventional cats. Ocelot and lion DNAs were essentially negative for these sequences.

Reiteration frequency of feline type C viral genomes in homologous and heterologous host cell DNA.

Differences in the multiplicity of proviral sequences of two feline endogenous viruses, RD-114 and FeLV, in homologous and heterologous host DNA were examined by viral cDNA hybridization using cellular DNA fractionated with respect to reiteration frequency. The endogenous proviral DNAs in cat cells were fractionated predominantly with intermediate-repeated sequences, while RD-114 proviral DNA in virus-infected human cells was fractionated together with unique-sequence DNA. These differences were consistent with the results of kinetic analysis using unfractionated DNA.