J. Fuska

Antitrichomonadal and other biological effects of sugar hydrazones and phenylosazones

When investigating the possible antitrichomodal effect of 41 phenylsazones, nitro-and dinitrophenylhydrazones a significant inhibitory effect on multiplication ofTrichomonas foetus could be detected in 14 derivatives; eight derivatives were ineffective, other derivatives exhibited only a alight effect. The inhibitory effect of most compounds increased after acetylation. Toxicity of seven effective compounds was determinedin vivo. Mutagenicity of these compounds was followed with microorganisms and their cytotoxicity with tumor EAC cells. Two of the effective compounds exhibited also a significant mutagenic effect, three of eight compounds had a pronounced cytotoxic effect, three of eight compounds had a pronounced cytotoxic effectin vitro on the EAC cells, in which they inhibited mainly the incorporation of adenine.

The effect of palitantin, a metabolite ofPenicillium frequentans onLeishmania brasiliensis

A compound with an antiprotozoal effect againstLeishmania brasiliensis was isolated from the submerged culture ofPenicillium frequentans 60A/7 which was collected in this country. The active compound was characterised as palitantin, a metabolite of several strains ofPenicillium the antibiotic activity of which has not yet been reported. The investigated strain produced 200–500mg of palitantin per liter of medium during submerged cultivation and also a small quantity of frequentin: 10–20mg per liter. Frequentin has no activity againstLeishmania brasiliensis and several other investigated protozoa.