Kindi Farabi

Activity of Cytotoxic Flavanoids against a P-388 Murine Leukemia Cell Line from the Stem Bark of Aglaia elliptica (Meliaceae)

800x600 Two mixture s of flavanoid compounds ( 1 and 2 ), a mixture of catechin ( 1a ) and epicatechin ( 1b ) , and a mixture of gallocatechin ( 2a ) and epigallocatechin ( 2b ) , were isolated from the active fraction of the stem bark of Aglaia elliptica methanol extract. The chemical structure of the compounds w as identified with spectroscopic data , including UV, IR, NMR ( 1 H, 13 C, DEPT 135°, HMQC, HMBC, 1 H- 1 H COSY) , and MS, and additionally compared with previously reported spectral data. All compounds were evaluated for their cytotoxic effects against P-388 murine leukemia cells. Compound 2 showed cytotoxicity against the P-388 murine leukemia cell , with an IC 50 value of 7.79 µg/mL, but compound 1 wa s found not to be active ( more than 100 µg/mL ) . Normal 0 false false false EN-ID X-NONE X-NONE

Cytototoxic constituents from the bark of Chisocheton cumingianus (Meliaceae).

Abstract A new lanostane-type triterpenoid, 3β-hydroxy-25-ethyl-lanost-9(11),24(24′)-diene (1), along with 3β-hydroxy-lanost-7-ene (2) and β-sitosterol-3-O-acetate (3) was isolated from the stem bark of C. cumingianus. The chemical structure of the new compound was elucidated on the basis of spectroscopic data. All of the compounds were evaluated for their cytotoxic effects against P-388 murine leukemia cells. Compounds 1-3 showed cytotoxicity against P-388 murine leukemia cells with IC50 values of 28.8 ± 0.10, 4.29 ± 0.03, and 100.18 ± 0.16 μg/ml, respectively.

Cytotoxic Steroids from the Bark of Aglaia argentea (Meliaceae)

The study aimed to find a potential anticancer agent by isolating and identifying the chemical structure of compounds from Aglaia argentea and testing their cytotoxic effects against P-388 murine leukimia cells. Five steroids – stigmast-5-en-3β-ol (β-sitosterol) (1), stigmast-5-en-3β-ol-3β-oleate (β-sitosterol oleate) (2), stigmast-5-en-3β-ol-3-O-(6′-O-oleoyl)-β-D-glucopyranoside (sitoindoside II) (3), stigmast-5-en-3β-ol-3-O-β-D-glucopyranoside (β-sitosterol glucoside) (4), stigmast-5,22-dien-3β-ol-3-O-β-D-glucopyranoside (stigmasterol glucoside) (5) – were isolated from the bark of Aglaia argentea. The chemical structures of 1-5 were identified with spectroscopic data, including IR, NMR (1H, 13C, DEPT 135°, HMQC, HMBC, 1H-1H COSY) and HRTOFMS, as well as by comparing with previously reported spectral data. All compounds were evaluated for their cytotoxic effects against P-388 murine leukemia cells. Compounds 1-5 showed cytotoxicity against P-388 murine leukemia cell with IC50 values of 12.45 ± 0.050, 85.25 ± 0.050, >100, 52.27 ± 0.031 and 62.52 ± 0.076 μg/mL, respectively.