Luiz Juliano Valério Geron

Recuperação Fecal de Indicadores Internos Avaliados em Ruminantes

This experiment was carried out to assess the fecal recuperation of the following internal markers: acid insoluble ash (AIA), acid detergent insoluble ash (ADIA), indigestible acid detergent fiber (IADF), indigestible neutral detergent fiber (INDF), and the apparent digestibility of dry matter (DM) and organic matter (OM). Four castrated sheep were used, fed with four levels of substitution of corn by cassava by-product meal. The feed samples, orts and feces were incubated in situ for 192 hours in three Holstein cows with ruminal cannula to determine the indigestible neutral detergent fiber and indigestible acid detergent fiber. The fecal recuperation of AIA and INDF were not different from 100% and had values for the digestibility coefficient of DM and OM similar to those obtained by the total feces collection method. The fecal recuperation of ADIA and IADF differed from 100%.

Substituição do milho pela farinha de varredura de mandioca (Manihot esculenta Crantz) em rações de ovinos: consumo, digestibilidade, balanços de nitrogênio e energia e parâmetros ruminais

This work was carried-out to study the effect of substitution levels of corn (COR) by cassava by-product flour (CPF) (25, 50, 75 and 100%) in the ration for sheep on voluntary intake, nutrients apparent digestibility and nitrogen (NB) and energy (EB) balance, pH and ammonia concentration (N-NH3) in ruminal liquid. Four male, castrated sheep, without defined race, with medium weight of 41.4 AW, in trial of digestibility were used, using the method of total collect feces and urine. The animals were kept in cages of metabolism in 4x4 latin square design. The ruminal liquid was obtained by means of probe esofagic in the times 0, 2, 4, 6 and 8 hours (after feeding). There was no effect of substitution levels on intake of dry matter (DM), organic matter (OM), neutral detergent fiber (NDF), energy and nitrogen (N) and for apparent digestibility of DM, OM, CP, NDF, starch and gross energy (GE) and for NB and TDN levels. The intake of starch and GE presented quadratic increase until the level of 75% CPM in the ration. The concentration of the ammonia nitrogen and the pH of ruminal liquid did not differ in function of the substitution levels of the COR to CPM. It was concluded that CPM can totally substitute COR in the sheep ration.

Digestibilidade e características ruminais de dietas com diferentes níveis de concentrado para bubalinos e bovinos

The objective of this trial was to evaluate three different concentrate levels (CL) (23, 43 and 63%) on intake, nutrient digestibility, ruminal metabolism, and microbial efficiency in Holstein bovines and Murrah buffaloes (Bubalus bubalis) fed diets containing ground corn and dry cassava by-product as energy sources. Three Holstein bovines and three Murrah buffaloes averaging, respectively, 473±54 kg and 400±33 kg of body weight (BW), fitted with ruminal and duodenal cannulas were blocked by species and randomly assigned to two replicated 3 x 3 Latin squares with a factorial arrangement of treatments. Chromium oxide was used as duodenal and fecal flow marker. Intake of dry matter (DM) was lower on bubalines (1.40% of BW) than on bovines (1.63% of BW). The greatest CP intake was of 10.09 g/kg BW0.75 for bovines and 8.63 g/kg BW0.75 for bubalines with 44.4% of concentrate in the diet while the highest starch intake was of 32.83 g/kg BW 0.75 for bovines and 28.38 g/kg BW 0.75 for bubalines with 61.69% of concentrate in the diet. There were no effects of CL and species on total tract digestibility, ruminal digestibility, and intestinal digestibility of nutrients with the exception of starch and CP. Apparent total tract digestibility of starch was greatest on bovines (95.7 vs. 85.8%); ruminal starch digestibility was also greatest on bovines (87.4% vs. 81.9%) and that increased linearly when the CL was increased in the diet. It was observed lower ruminal digestibility (13.2 vs. 27.5%) and higher intestinal digestibility (86.8 vs. 72.5%) of CP on bovines compared to bubalines in this trial. Ruminal pH averaged 6.7 on bubalines and was greater that than on bovines (6.3). Ruminal ammonia concentration differed across treatments on bubalines but not on bovines whereas microbial efficiency was not affected either by dietary CL or by species.

Coeficiente de digestibilidade e características ruminais de bovinos alimentados com rações contendo resíduo de cervejaria fermentado

The objective of the present study was to evaluate the partial and total digestibility coefficient (DC) of the nutrients, ruminal pH, ammonia nitrogen (N-NH3) concentration in the ruminal fluid and microbial efficiency synthesis in bovines fed rations containing 0, 8, 16 and 24% of brewers grains. The brewer grains were ensiled in surface silo (5,000 kg). Four Holstein cattle (480 ± 100 kg of body weight) fitted with ruminal and duodenal cannulas, were distributed to 4 × 4 Latin square design. The mean DM intake of animals was 1.7% BW. Total and ruminal DC of the nutrients did not differ among the levels of BG used in the rations. The levels of BG in the rations showed a linearly increased on the intestinal DC dry matter and TC, as % of that reached the compartment. There was no effect of levels of BG in rations on pH and N-NH3 concentration in the ruminal fluid. The levels of RCF in diets did not affect the microbial efficiency synthesis in g N-Mic/kg of rumen apparent degradable organic matter and rumen truly degradable organic matter. The inclusion of up to 24% of BG (%DM) cattle feed does not alter the processes of rumen fermentation and digestion of nutrients.

Digestibilidade e balanço de nitrogênio de rações com diferentes teores de proteína degradável no rúmen e milho moído como fonte de amido em ovinos

Four castrated sheep averaging 38.5 kg of body weight were randomly assigned to a 4 x 4 Latin square to evaluate the apparent digestibility of nutrients and nitrogen balance (NB) on diets containing different levels of rumen degradable protein (RDP): 46, 50, 54, and 58% and ground corn. The four experimental diets were composed by 75% of forage (oat hay) and 25% of concentrate containing ground corn (GC), soybean meal (SM), cottonseed meal (CM), fish meal (FM), urea, and mineral salt. No significant differences were observed on the intakes of DM, OM, CP, starch, NDF, and ADF by increasing the dietary RDP levels. Nitrogen intake (NI), urinary N excretion, fecal N excretion, and NB expressed as % NI averaged, respectively, 31.68 g/d, 6.34 g/d, 11.31 g/d, and 47.27% and did not differ across diets. Similarly, apparent total tract digestibilities of DM, OM CP, NDF, and ADF averaged, respectively, 71.90, 73.00, 79.70, 61.10, and 52.00% and did not differ when the RDP level was increased in the diet. It can be concluded that both digestibility of nutrients and NB were not affected by different dietary RDP levels under the conditions of this trial.

Avaliação de cinco híbridos de milho (Zea mays, L.) em diferentes estádios de maturação: 3. Composição químico-bromatológica

The objective of this study was evaluate, of the chemical composition of five corn (Zea mays L.) hybrids Pioneer: P32R21, P30R07, P3041, P30F33 and P30F80, at different maturity stages. The treatments were used in plots of 7 x 8 m in a randomized block design, with four replicates. The samples collected of corn hybrid were adopted trying to estimate phases of maturity of the plant: 30, 34, 38, 42 and 46% of dry matter (DM)). For stages of maturity, proceed separation stem + sheath, lamina leaf, and grain at analysis chemicals. In fraction grain, was determinated levels total nitrogen and starch in the different maturation stages. In the fractions of leaf lamina and stem + sheath, levels the following analysis chemicals was determinated: acid detergent fiber (ADF), neuter detergent fiber (NDF), lignin, acid detergent insoluble nitrogen (ADIN) and total nitrogen. All the variables presented differences between the hybrids and days after seeding (DAS). With the advance of the maturity, observed a decrease of crude protein (CP) and of crude starch practicably not varied. For fraction lamina leaf, verification that, increased levels the dry matter at plant, and decreased the levels starch, CP and ADID, contrary the fraction stem + sheath, increased levels. The levels NDF, ADF and lignin the lamina leaf increased, but this nutrition at fraction stem + sheath the corn hybrid, practicably, not varied with advance the maturity of stages.

Substituição do Milho pela Farinha de Varredura (Manihot esculenta, Crantz) na Ração de Bezerros Holandeses.: 2. Digestibilidade e Valor Energético

Twenty Holstein intact calves, averaging 160 days old and initial live weight of 163 kg, were assigned to a completely randomized design to evaluate the effect of five replacement levels (0, 25, 50, 75 and 100% in dry matter basis) of corn for cassava meal on the intake and apparent digestibility of dry matter, crude protein, neutral detergent fiber, starch and gross energy of the experimental diets. There was no effect of replacement levels of corn for cassava meal on the dry matter intake. The increase of the dietary cassava meal levels linearly increased the apparent digestibility coefficient of dry matter, crude protein, starch, and gross energy, but it did not affect the neutral detergent fiber. The replacement of corn for cassava meal did not affected the dietary energy levels.

Substituição do Milho pela Farinha de Varredura (Manihot esculenta, Crantz) na Ração de Bezerros Holandeses.: 1. Desempenho e Parâmetros Sangüíneos

The objective of this project was to evaluate the effects of different replacement levels of corn for cassava meal (0, 25, 50, 75, and 100%, dry matter basis) on the dry matter intake and feed:gain ratio, daily weight gain and levels of blood hematocrit, glucose and urea. Thirty five intact Holstein calves, averaging 80 days old and initial live weight of 80 kg, were allotted to a randomized block design and fed concentrate, ad libitum, to reach the intake of 4 kg/day. Tifton 85 bermudagrass was fed as roughage. The final average weight was of 164 kg. The dry matter intake showed a linear behavior as the replacement levels increased. The feed:gain ratio was not affected by the replacement levels. The daily weight gain for the two first periods of 28 days (period 1 and 2, respectively) reduced as the replacement levels increased, but they were not affected in the last 28 days period (period 3), and the respective values ranged from 0.93 to 0.68, 1.10 to 0.89, and 1.09 kg/day. Levels of hematocrit, glucose and urea were not affected by the replacement of corn for cassava meal.

Proteína degradável no rúmen associada a fontes de amido de alta ou baixa degradabilidade: digestibilidade in vitro e desempenho de novilhos em crescimento

The objective of this study was to evaluate the effects of increasing levels of rumen-degradable protein (RDP = 47, 52, 57 and 62%) associated to starch sources of low (corn) and high (cassava by-product (CBP) ruminal degradability on in vitro DM digestibility (IVDMD) and average daily gain of growing steers. In vitro DM digestibility was determined by using the Tilley and Terry (1963) technique. The in vitro data was submitted to analysis of variance and regression analysis in function of dietary RDP levels. The in vivo trial was conducted using 32 crossbred steers (Nellore x Red Angus) averaging 275 kg of body weight and fed diets with 50:50 forage:concentrate ratio. Animals were housed in pairs and assigned to treatments according to a completely randomized design. No effect of increasing RDP levels was observed on IVDMD on diets supplemented with corn indicating no lack of nitrogen. Thus, corn digestibility appeared to be the limiting factor on IVDMD. A significant quadratic effect was found for IVDMD by increasing the RDP levels on diets supplemented with CBP suggesting that a better synchronization between energy availability and ammonia release improved microbial growth, which increased the efficiency of energy and RDP utilization on diets with high RDP. It was not observed effect of different RDP levels associated to a starch source rapidly degraded in the rumen on DM intake, average daily gain, and feed conversion on feedlot steers.

Chemical composition, fermentation, in vitro digestibility and in situ degradability of sugar cane silages with Lactobacillus, urea and agricultural byproduct

The objective of this study was to evaluate the sugarcane silage in relation to chemical composition, pH, total losses, aerobic stability, in vitro digestibility of dry matter (IVDDM) and effective degradability (ED) of bovine and buffaloes under the following treatments (on a dry matter (DM) basis): sugarcane silage control (control), with addition of 3.3% urea; with addition of Lactobacillus buchneri (inoculate); with inoculate + 3.3% urea; with inoculate + 25.0% soybean hull, and with inoculate + 25.0% cassava byproduct meal. The experiment was analyzed through a completely randomized design with three replications, and for DM ED, a double 4 × 4 Latin square with a 4 × 2 factorial arrangement was used. The sugarcane variety was the RB-855156 (precocious). The urea increased the protein level and reduced neutral and acid detergent fiber and lignin fractions. Treatments with inoculate + cassava byproduct and inoculate + soybean hull increased DM levels. Bovines presented higher IVDDM than buffaloes. pH values varied from 3.1 to 4.0. Mean total losses observed were 7.8%. Treatments with inoculate + cassava and inoculate + soybean hulls resulted in larger aerobic stability with 61 and 81 hours, respectively, in relation to silages control (24 h) and control with 3.3% urea (33 h). A higher DM ED was verified for silages with inoculate + control and inoculate + soybean hulls at 2%/h passage rates and higher neutral detergent fiber ED for the treatment of control + inoculate + soybean hulls at 2 and 5%/h. Silages treated with urea, L. buchneri or agricultural byproduct plus inoculate presented better quality pattern.