Maija Vuoriluoto
Aalto University
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Featured researches published by Maija Vuoriluoto.
ACS Applied Materials & Interfaces | 2016
Maija Vuoriluoto; Hannes Orelma; Baolei Zhu; Leena-Sisko Johansson; Orlando J. Rojas
We passivated TEMPO-oxidized cellulose nanofibrils (TOCNF) toward human immunoglobulin G (hIgG) by modification with block and random copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA). The block copolymers reversibly adsorbed on TOCNF and were highly effective in preventing nonspecific interactions with hIgG, especially if short PDMAEMA blocks were used. In such cases, total protein rejection was achieved. This is in contrast to typical blocking agents, which performed poorly. When an anti-human IgG biointerface was installed onto the passivated TOCNF, remarkably high affinity antibody-antigen interactions were observed (0.90 ± 0.09 mg/m(2)). This is in contrast to the nonpassivated biointerface, which resulted in a significant false response. In addition, regeneration of the biointerface was possible by low pH aqueous wash. Protein A from Staphylococcus aureus was also utilized to successfully increase the sensitivity for human IgG recognition (1.28 ± 0.11 mg/m(2)). Overall, the developed system based on TOCNF modified with multifunctional polymers can be easily deployed as bioactive material with minimum fouling and excellent selectivity.
Journal of Physical Chemistry B | 2015
Maija Vuoriluoto; Hannes Orelma; Leena-Sisko Johansson; Baolei Zhu; Mikko Poutanen; Andreas Walther; Janne Laine; Orlando J. Rojas
Block copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA) with varying block sizes were synthesized by consecutive reversible addition-fragmentation chain transfer (RAFT) polymerization and then exposed to cellulose substrates with different anionic charge density. The extent and dynamics of quaternized PDMAEMA-b-POEGMA adsorption on regenerated cellulose, cellulose nanofibrils (CNF), and (2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO)-oxidized cellulose nanofibrils (TOCNF) was determined by using electromechanical and optical techniques, namely, quartz crystal microbalance (QCM-D) and surface plasmon resonance (SPR), respectively. PDMAEMA-b-POEGMA equilibrium adsorption increased with the anionic charge of cellulose, an indication of electrostatic interactions. Such an observation was further confirmed by atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). Depending on their architecture, adsorption on TOCNF of some of the PDMAEMA-b-POEGMA copolymers produced a significant reduction in QCM frequency, as expected from large mass uptake, while surprisingly, other copolymers induced the opposite effect. This latter, remarkable behavior was ascribed to coupled water expulsion from the interface upon charge neutralization of anionic surface sites with adsorbing cationic polymer segments. These observations were further investigated with SPR and QCM-D measurements using deuterium oxide solvent exchange to determine the amount of coupled water at the TOCNF-block copolymer interface. Finally, random copolymers with similar composition adsorbed to a larger extent compared to the respective block copolymers, revealing the effect of adsorbed loops and tails as well as hydration.
Carbohydrate Polymers | 2014
Anni Sorvari; Tapio Saarinen; Sanna Haavisto; Juha Salmela; Maija Vuoriluoto; Jukka Seppälä
Carboxymethylcellulose (CMC) and xanthan gum were studied as dispersants for microfibrillated cellulose (MFC) suspension using a rotational rheometer and imaging methods. The imaging was a combination of photography and optical coherence tomography (OCT). Both polymers dispersed MFC fibers, although CMC was more effective than xanthan gum. The negatively charged polymer chains increased the viscosity of the suspending medium and acted as buffers in between the negatively charged fibers. This behavior decreased the number and strength of contacts between the fibers and subsequently dispersed the flocs. The stronger separation of the fibers was reflected in the frequency sweep where the MFC/polymer suspensions had lower gel strength than pure MFC suspension. Dispersing effect was also observed in the flow measurements, where the floc size was more uniform with polymers in the decelerating flow and after long, slow constant shear, which normally induces a heterogeneous structure with large flocs into the MFC suspension.
Biomacromolecules | 2017
Maija Vuoriluoto; Hannes Orelma; Meri J. Lundahl; Maryam Borghei; Orlando J. Rojas
We demonstrate benzophenone (BP) conjugation via amine-reactive esters onto oxidized cellulosic fibers that were used as precursors, after microfluidization, of photoactive cellulose nanofibrils (CNF). From these fibrils, cellulose I filaments were synthesized by hydrogel spinning in an antisolvent followed by fast biradical UV cross-linking. As a result, the wet BP-CNF filaments retained extensively the original dry strength (a remarkable ∼80% retention). Thus, the principal limitation of these emerging materials was overcome (the wet tensile strength is typically <0.5% of the value measured in dry conditions). Subsequently, antihuman hemoglobin (anti-Hb) antibodies were conjugated onto residual surface carboxyl groups, making the filaments bifunctional for their active groups and properties (wet strength and bioactivity). Optical (surface plasmon resonance) and electroacoustic (quartz crystal microgravimetry) measurements conducted with the bifunctional CNF indicated effective anti-Hb conjugation (2.4 mg m-2), endowing an excellent sensitivity toward Hb targets (1.7 ± 0.12 mg m-2) and negligible nonspecific binding. Thus, the anti-Hb biointerface was deployed on filaments that captured Hb efficiently from aqueous matrices (confocal laser microscopy of FITC-labeled antibodies). Significantly, the anti-Hb biointerface was suitable for regeneration, while its sensitivity and selectivity in affinity binding can be tailored by application of blocking copolymers. The developed bifunctional filaments based on nanocellulose offer great promise in detection and affinity binding built upon 1D systems, which can be engineered into other structures for rational use of material and space.
RSC Advances | 2016
Hannes Orelma; Maija Vuoriluoto; Leena-Sisko Johansson; J.M. Campbell; Ilari Filpponen; Markus Biesalski; Orlando J. Rojas
A method for preparing photo-crosslinkable cellulose nanofibrils (CNF) was investigated. Benzophenone (BP), a UV-radical crosslinker, was chemically grafted to TEMPO-oxidized wood fibers in water/DMSO medium. This resulted in a reduction of carboxyl group content together with an increase of the number density of amide linkages. The BP-functionalized fibres were then microfluidized into TEMPO-oxidized CNF (TOCNF). As evidence of the crosslinking performance, the films of BP-activated TOCNF displayed improved water resistance upon UV curing (even under high energy sonication). In addition to improving wet strength the method is suitable for further modification of CNF, either by utilizing the remaining free carboxyl groups or via photochemical grafting of other substances onto the CNF structure.
ACS Applied Materials & Interfaces | 2016
Satu Rajala; Tuomo Siponkoski; Essi Sarlin; Marja Mettänen; Maija Vuoriluoto; Arno Pammo; Jari Juuti; Orlando J. Rojas; Sami Franssila; Sampo Tuukkanen
Archive | 2017
Maija Vuoriluoto
international conference on nanotechnology | 2016
Maija Vuoriluoto; Meri J. Lundahl; Mariko Ago; Maryam Borghei; Gisela Cunha; Hannes Orelma; Ilari Filpponen; Orlando J. Rojas
Archive | 2015
Satu Rajala; Maija Vuoriluoto; Orlando J. Rojas; Sami Franssila; Sampo Tuukkanen
American Chemical Society National Meeting & Exposition | 2015
Maija Vuoriluoto; Hannes Orelma; Mikko Poutanen; Andreas Walther; Janne Laine; Orlando J. Rojas