Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Natasha M. Agramonte is active.

Publication


Featured researches published by Natasha M. Agramonte.


Journal of Medical Entomology | 2012

Aedes aegypti (Diptera: Culicidae) Biting Deterrence: Structure-Activity Relationship of Saturated and Unsaturated Fatty Acids

Abbas Ali; Charles L. Cantrell; Ulrich R. Bernier; Stephen O. Duke; John C. Schneider; Natasha M. Agramonte; Ikhlas A. Khan

ABSTRACT In this study we evaluated the biting deterrent effects of a series of saturated and unsaturated fatty acids against Aedes aegypti (L), yellow fever mosquito (Diptera: Culicidae) using the K &D bioassay module system. Saturated (C6:0 to C16:0 and C18:0) and unsaturated fatty acids (C11:1 to C14:1, C16:1, C18:1, and C18:2) showed biting deterrence index (BDI) values significantly greater than ethanol, the negative control. Among the saturated fatty acids, mid chain length acids (C10:0 to C13:0) showed higher biting deterrence than short (C6:0 to C9:0) and long chain length acids (C14:0 to C18:0), except for C8:0 and C16:0 that were more active than the other short and long chain acids. The BDI values of mid chain length acids (C10:0 to C13:0) were not significantly less than N, N-diethyl-meta-toluamide (DEET), the positive control. Among the unsaturated fatty acids, C11:1 showed the highest activity (BDI = 1.05) and C18:2 had the lowest activity (BDI = 0.7). In C11:1, C12:1, and C14:1 BDI values were not significantly less than DEET. After the preliminary observations, residual activity bioassays were performed on C11:0, C12:0, C11:1, and C12:1 over a 24-h period. All the fatty acids (C11:0, C12:0, C11:1, and C12:1) and DEET showed significantly higher activity at all test intervals than the solvent control. At treatment and 1-h posttreatment, all fatty acids showed proportion not biting (PNB) values not significantly less than DEET. At 3-, 6-, and 12-h posttreatment, all fatty acids showed PNB values significantly greater than DEET. At 24-h posttreatment, only the PNB value for C12:0 was significantly higher than DEET. The dose-responses of C12:0 and DEET were determined at concentrations of 5–25 nmol/cm2. As in the residual activity bioassays, the PNB values for C12:0 and DEET at 25 nmol/cm2 were not significantly different. However, at lower concentrations, the PNB values for C12:0 were significantly greater than DEET. These results clearly indicate that mid chain length fatty acids not only have levels of biting deterrence similar to DEET at 25 nmol/cm2 in our test system, but also appeared to be more persistent than DEET. In contrast, in vivo cloth patch assay system showed that the mid-chain length fatty acids, C11:0, C11:1 C12:0, and C12:1 had minimum effective dose (MED) values greater than DEET against Ae. aegypti and their relative repellency varied according to species tested. The MED values of 120 (C11:0), 145 (C12:0) and 116 (C11:1) nmol/cm2 against Anopheles quadrimaculatus Say, indicated that these acids were not as potent as DEET with a MED of 54 nmol/cm2. The MED ratio of the C11:0 and C11:1 for all three mosquito species indicated the C11 saturated and unsaturated acids as more repellent than their corresponding C12:0 and C12:1 homologues.


Molecules | 2013

Chemical Composition, Antifungal and Insecticidal Activities of Hedychium Essential Oils

Hamidou F. Sakhanokho; Blair J. Sampson; Nurhayat Tabanca; David E. Wedge; Betül Demirci; K. H.C. Baser; Ulrich R. Bernier; Maia Tsikolia; Natasha M. Agramonte; James J. Becnel; Jian Chen; Kanniah Rajasekaran; James M. Spiers

The antimicrobial properties of essential oils have been documented, and their use as “biocides” is gaining popularity. The aims of this study were to analyze the chemical composition and assess the biological activities of Hedychium essential oils. Oils from 19 Hedychium species and cultivars were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) techniques. The antifungal and insecticidal activities of these oils were tested against Colletotrichum acutatum, C. fragariae, and C. gloeosporioides, and three insects, the azalea lace bug (Stephanitis pyrioides), the yellow fever mosquito (Aedes aegypti), and the red imported fire ant (Solenopsis invicta). Hedychium oils were rich in monoterpenes and sesquiterpenes, especially 1,8-cineole (0.1%–42%), linalool (<0.1%–56%), α-pinene (3%–17%), β-pinene (4%–31%), and (E)-nerolidol (0.1%–20%). Hedychium oils had no antifungal effect on C. gloeosporioides, C. fragariae, and C. acutatum, but most Hedychium oils effectively killed azalea lace bugs. The oils also show promise as an adult mosquito repellent, but they would make rather poor larvicides or adulticides for mosquito control. Hedychium oils acted either as a fire ant repellent or attractant, depending on plant genotype and oil concentration.


Journal of Medical Entomology | 2015

Essential Oils of Echinophora lamondiana (Apiales: Umbelliferae): A Relationship Between Chemical Profile and Biting Deterrence and Larvicidal Activity Against Mosquitoes (Diptera: Culicidae)

Abbas Ali; Nurhayat Tabanca; Gülmira Özek; T. Özek; Zeki Aytaç; Ulrich R. Bernier; Natasha M. Agramonte; K. Hüsnü Can Başer; Ikhlas A. Khan

ABSTRACT The essential oils from the flower, leaf, and stem of Echinophora lamondiana B.Yildiz et Z.Bahcecioglu were analyzed by gas chromatography—flame ionization detection and gas chromatography—mass spectrometry. In total, 41, 37, and 44 compounds were identified, which accounted for 98.0, 99.1, and 97.0% of flower, leaf, and stem essential oils, respectively. The monoterpenic hydrocarbons were found to be high in all samples of the essential oils. The major components of essential oils from flower, leaf, and stem of E. lamondiana were &dgr;-3-carene (61.9, 75.0, and 65.9%, respectively), &agr;-phellandrene (20.3, 14.1, and 12.8%, respectively), and terpinolene (2.7, 3.3, and 2.9%, respectively). Flower and leaf essential oils and terpinolene produced biting deterrence similar to 25 nmol/cm2 N, Ndiethyl-meta-toluamide (DEET; 97%) against Aedes aegypti (L.) and Anopheles quadrimaculatus Say. Compounds (+)-&dgr;-3-carene, (R)-(-)-&agr;-phellandrene, and water-distilled essential oils were significantly less repellent than DEET. Among essential oils, leaf oil was the least toxic of the oils, with an LC50 value of 138.3 ppm, whereas flower essential oil killed only 32% larvae, and no mortality of stem oil at highest tested dosages against Ae aegypti was observed. Terpinolene and &agr;-phellandrene showed higher toxicity than &dgr;-3-carene in both the species. In contrast to Ae. aegypti, all the essential oils showed toxicity in An. quadrimaculatus, and toxicity was higher in leaf oil than the other two oils. These results could be useful in finding new, safe, and more effective natural biopesticides and biting deterrent or repellents against Ae. aegypti.


Pesticide Biochemistry and Physiology | 2013

Insecticidal, Repellent and Fungicidal Properties of Novel Trifluoromethylphenyl Amides

Maia Tsikolia; Ulrich R. Bernier; Monique R. Coy; Katelyn C. Chalaire; James J. Becnel; Natasha M. Agramonte; Nurhayat Tabanca; David E. Wedge; Gary G. Clark; Kenneth J. Linthicum; Daniel R. Swale; Jeffrey R. Bloomquist

Twenty trifluoromethylphenyl amides were synthesized and evaluated as fungicides and as mosquito toxicants and repellents. Against Aedes aegypti larvae, N-(2,6-dichloro-4-(trifluoromethyl)phenyl)-3,5-dinitrobenzamide (1e) was the most toxic compound (24 h LC50 1940 nM), while against adults N-(2,6-dichloro-4-(trifluoromethyl)phenyl)-2,2,2-trifluoroacetamide (1c) was most active (24 h LD50 19.182 nM, 0.5 μL/insect). However, the 24 h LC50 and LD50 values of fipronil against Ae. aegypti larvae and adults were significantly lower: 13.55 nM and 0.787 × 10(-4) nM, respectively. Compound 1c was also active against Drosophila melanogaster adults with 24 h LC50 values of 5.6 and 4.9 μg/cm(2) for the Oregon-R and 1675 strains, respectively. Fipronil had LC50 values of 0.004 and 0.017 μg/cm(2) against the two strains of D. melanogaster, respectively. In repellency bioassays against female Ae. aegypti, 2,2,2-trifluoro-N-(2-(trifluoromethyl)phenyl)acetamide (4c) had the highest repellent potency with a minimum effective dosage (MED) of 0.039 μmol/cm(2) compared to DEET (MED of 0.091 μmol/cm(2)). Compound N-(2-(trifluoromethyl)phenyl)hexanamide (4a) had an MED of 0.091 μmol/cm(2) which was comparable to DEET. Compound 4c was the most potent fungicide against Phomopsis obscurans. Several trends were discerned between the structural configuration of these molecules and the effect of structural changes on toxicity and repellency. Para- or meta- trifluoromethylphenyl amides with an aromatic ring attached to the carbonyl carbon showed higher toxicity against Ae. aegypti larvae, than ortho- trifluoromethylphenyl amides. Ortho- trifluoromethylphenyl amides with trifluoromethyl or alkyl group attached to the carbonyl carbon produced higher repellent activity against female Ae. aegypti and Anopheles albimanus than meta- or para- trifluoromethylphenyl amides. The presence of 2,6-dichloro- substitution on the phenyl ring of the amide had an influence on larvicidal and repellent activity of para- trifluoromethylphenyl amides.


PLOS Neglected Tropical Diseases | 2017

Pyrethroid resistance alters the blood-feeding behavior in Puerto Rican Aedes aegypti mosquitoes exposed to treated fabric

Natasha M. Agramonte; Jeffrey R. Bloomquist; Ulrich R. Bernier

Emerging insecticide resistance is a major issue for vector control. It decreases the effectiveness of insecticides, thereby requiring greater quantities for comparable control with a net increase in risk of disease resurgence, product cost, and damage risk to the ecosystem. Pyrethroid resistance has been documented in Puerto Rican populations of Aedes aegypti (L.) mosquitoes. In this study, topical toxicity of five insecticides (permethrin, etofenprox, deltamethrin, DDT, transfluthrin) was determined for susceptible (Orlando—ORL) and resistant (Puerto Rico—PR) strains of Ae. aegypti. Resistance ratios were calculated using LD50 values, and high resistance ratios for permethrin (112) and etofenprox (228) were observed for the Puerto Rico strain. Behavioral differences in blood-feeding activity for pyrethroid-resistant and pyrethroid-susceptible strains of Ae. aegypti when exposed to pyrethroid-treated cloth were also explored. Strains were exposed for 15 min to a range of concentrations of pyrethroid-treated uniform fabric in a cage that contained 60 female Ae. aegypti mosquitoes. Interestingly, the resistance ratios for blood-feeding were similar for permethrin (61) and etofenprox (70), but were lower than their respective resistance ratios for topical toxicity, suggesting that knockdown resistance was the primary mechanism of resistance in the blood feeding assays. Results showed a rightward shift in the dose-response curves for blood-feeding that indicated higher concentrations of pyrethroids were necessary to deter blood-feeding behavior in the pyrethroid-resistant Puerto Rican strain of Ae. aegypti.


Natural Product Research | 2017

Repellency of the Origanum onites L. essential oil and constituents to the lone star tick and yellow fever mosquito

J. F. Carroll; Betül Demirci; Matthew Kramer; Ulrich R. Bernier; Natasha M. Agramonte; K. Husnu Can Baser; Nurhayat Tabanca

Abstract The oregano, Origanum onites L., essential oil (EO) was tested in laboratory behavioural bioassays for repellent activity against Amblyomma americanum (L.) and Aedes aegypti (L.). The O. onites EO was characterised using GC-FID and GC-MS. Carvacrol (75.70%), linalool (9.0%), p-cymene (4.33%) and thymol (1.9%) were the most abundant compounds. At a concentration of 0.413 mg oil/cm2 of filter paper, O. onites EO repelled 100% of the ticks tested and at 0.103 mg oil/cm2 of filter paper, 66.7% of the ticks were repelled. At 0.075 mg oil/cm2 filter paper, thymol repelled 66.7% of the ticks compared to 28.7% by carvacrol at that same concentration. Against Ae. aegypti, O. onites EO was repellent at the minimum effective dosage (MED) of 0.011 (±0.00) mg/cm2 in the cloth patch assay compared to the reference control, N,N-dimethyl-3-methylbenzamide (DEET) with a MED = 0.007 ± (0.003) mg/cm2.


Journal of The Saudi Pharmaceutical Society | 2017

Rhanterium epapposum Oliv. essential oil: Chemical composition and antimicrobial, insect-repellent and anticholinesterase activities

Betül Demirci; Hasan Soliman Yusufoglu; Nurhayat Tabanca; Halide Edip Temel; Ulrich R. Bernier; Natasha M. Agramonte; Saleh I. Alqasoumi; Adnan J. Al-Rehaily; K. H.C. Baser; Fatih Demirci

The essential oil (EO) of the aerial parts of Rhanterium epapposum Oliv. (Asteraceae), was obtained by hydrodistillation. The oil was subsequently analyzed by both GC-FID and GC-MS, simultaneously. Forty-five components representing 99.2% of the oil composition were identified. The most abundant compounds were camphene (38.5%), myrcene (17.5%), limonene (10.1%) and α-pinene (8.7%). Referring to the ethnobotanical utilization, an insecticidal assay was performed, where the oil repelled the yellow fever mosquito Aedes aegypti L. at a minimum effective dose (MED of 0.035 ± 0.010 mg/cm2) compared to the positive control DEET (MED of 0.015 ± 0.004 mg/cm2). Additionally, the in vitro antimicrobial activity against a panel of pathogens was determined using a microdilution method. The acetyl- and butyrylcholine esterase inhibitory activities were measured using the colorimetric Ellman method. The bioassay results showed that the oil was rather moderate in antimicrobial and cholinesterase inhibitions when compared to the standard compounds.


Journal of Medical Entomology | 2016

Comparative Evaluation of a Silicone Membrane as an Alternative to Skin for Testing Mosquito Repellents.

Natasha M. Agramonte; Salvador Gezan; Ulrich R. Bernier

Abstract Repellents prevent mosquito bites and help reduce mosquito-borne disease, a global public health issue. Laboratory-based repellent bioassays predict the ability of compounds to deter mosquito feeding, but the variety of repellent bioassays and statistical analysis methods makes it difficult to compare results across methodologies. The most realistic data are collected when repellents are applied on the skin; however, this method exposes volunteers to chemicals and mosquito bites. Silicone membranes were investigated as an alternative to human skin in assays of repellent efficacy. Results from module system bioassays conducted in vitro with a silicone membrane were compared with in vivo bioassays conducted with N,N-diethyl-3-methylbenzamide (referred to as DEET), 1-piperidinecarboxylic acid 2-(2-hydroxyethyl)-1-methylpropylester (referred to as Picaridin), ethyl 3-[acetyl(butyl)amino]propanoate (referred to as IR3535), and para-menthane-3,8-diol (referred to as PMD) applied directly on the skin of the leg. No significant difference in mosquito feeding was found when comparing skin and volunteer-worn membrane controls using blood; however, feeding was significantly lower in unworn membrane controls using either 10% sucrose or blood, indicating that worn membranes are a possible surrogate for untreated human skin. Pooled data from six volunteers were used to generate dose–response curves of blood-feeding activity. Results from skin-applied repellents were modeled to determine if membranes could provide a predictive correlate for skin. Goodness-of-fit comparisons indicated that the nonlinear dose–response curves for the skin and membrane differed significantly for DEET and Picaridin, but did not differ significantly for IR3535 and PMD. With knowledge of the dose–response relationships and further modifications to this system, the membrane-based tests could be used for standardized repellent testing with infected vectors


Scientific Reports | 2018

Better than DEET Repellent Compounds Derived from Coconut Oil

J. J. Zhu; Steven C. Cermak; James A. Kenar; Gary J. Brewer; Kenneth F. Haynes; Dave Boxler; Paul D. Baker; Desen Wang; Changlu Wang; Andrew Y. Li; Rui-De Xue; Yuan Shen; Fei Wang; Natasha M. Agramonte; Ulrich R. Bernier; Jaires Gomes de Oliveira Filho; Lígia Miranda Ferreira Borges; Kristina Friesen; David B. Taylor

Hematophagous arthropods are capable of transmitting human and animal pathogens worldwide. Vector-borne diseases account for 17% of all infectious diseases resulting in 700,000 human deaths annually. Repellents are a primary tool for reducing the impact of biting arthropods on humans and animals. N,N-Diethyl-meta-toluamide (DEET), the most effective and long-lasting repellent currently available commercially, has long been considered the gold standard in insect repellents, but with reported human health issues, particularly for infants and pregnant women. In the present study, we report fatty acids derived from coconut oil which are novel, inexpensive and highly efficacious repellant compounds. These coconut fatty acids are active against a broad array of blood-sucking arthropods including biting flies, ticks, bed bugs and mosquitoes. The medium-chain length fatty acids from C8:0 to C12:0 were found to exhibit the predominant repellent activity. In laboratory bioassays, these fatty acids repelled biting flies and bed bugs for two weeks after application, and ticks for one week. Repellency was stronger and with longer residual activity than that of DEET. In addition, repellency was also found against mosquitoes. An aqueous starch-based formulation containing natural coconut fatty acids was also prepared and shown to protect pastured cattle from biting flies up to 96-hours in the hot summer, which, to our knowledge, is the longest protection provided by a natural repellent product studied to date.


Pesticide Biochemistry and Physiology | 2018

Insecticidal and repellent properties of novel trifluoromethylphenyl amides II

Maia Tsikolia; Ulrich R. Bernier; Natasha M. Agramonte; Alden S. Estep; James J. Becnel; Nurhayat Tabanca; Kenneth J. Linthicum; Aaron D. Gross; Patrick M. Guerin; Thomas Kröber; Jeffrey R. Bloomquist

This project focused on the design, synthesis, and testing of trifluoromethylphenyl amides (TFMPAs) as potential mosquitocides and repellents. Fourteen compounds were evaluated for toxicity against larvae and adults of Aedes aegypti. Several compounds were toxic against Aedes aegypti (larval, adult and feeding bioassays) and Drosophila melanogaster (glass-surface contact assay), but were much less toxic than fipronil, with toxicity ratios ranging from 100-fold in the larval assay to 100,000-fold for topical application to adult insects. In repellency bioassays to determine minimum effective dosage (MED), compound N-(2,6-dichloro-4-(trifluoromethyl)phenyl)-2,2,3,3,3-pentafluoropropanamide (7b) repelled Ae. aegypti females at lower concentration, 0.017 (±0.006) μmol/cm2, than N, N-diethyl-meta-toluamide (DEET) 0.026 (±0.005) μmol/cm2. 2-Chloro-N-(3-(trifluoromethyl)phenyl)acetamide (6a) performed better than DEET against two species of mosquitoes: it repelled Ae. aegypti females at 0.013 (±0.006) μmol/cm2 and Anopheles gambiae females (in a warm body repellent assay), at a standard exposure of 2 nmol/cm2. These studies revealed novel active structures that could further lead to compounds with better repellent activity.

Collaboration


Dive into the Natasha M. Agramonte's collaboration.

Top Co-Authors

Avatar

Ulrich R. Bernier

Agricultural Research Service

View shared research outputs
Top Co-Authors

Avatar

Nurhayat Tabanca

Agricultural Research Service

View shared research outputs
Top Co-Authors

Avatar

James J. Becnel

Agricultural Research Service

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar

David E. Wedge

United States Department of Agriculture

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Abbas Ali

University of Mississippi

View shared research outputs
Top Co-Authors

Avatar

Ikhlas A. Khan

University of Mississippi

View shared research outputs
Top Co-Authors

Avatar

Kenneth J. Linthicum

United States Department of Agriculture

View shared research outputs
Researchain Logo
Decentralizing Knowledge