Nejla Yardimci

Identification of Beet necrotic yellow vein virus in Lakes District: A Major Beet Growing Area in Turkey.

This study was conducted to determine the prevalence of Beet necrotic yellow vein virus (BNYVV) causal agent of rhizomania, in sugar beet cultivation areas in the Lakes District of Turkey, in 2006−2007. During surveys BNYVV suspected leaf, root, and soil samples were collected. Total of 203 soil samples were collected from different locations in the region. Sugar beet cultivar Kasandra plants were grown in these soil samples using bait plant techniques. In order to detect the structures of the vector protozoa Polymyxa betae Keskin in the root tissue of the bait plants, roots were stained with lactofuchsin and examined for the fungal cystosori under a light microscope. Presence of BNYVV was investigated using Double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA). ELISA test results showed that 85 samples were infected with BNYVV (41.87%). ELISA-positive samples for BNYVV were used in the mechanical inoculation studies. Test plants produced typical BNYVV symptoms. Fifty ELISA negative samples when tested by reverse transcription polymerase chain reaction (RT-PCR), BNYVV could be detected in 50% of these samples. The present study provides evidence for occurrence of BNYVV in a major beet growing area in Turkey based on biological, serological and molecular detection of the virus. RT-PCR method was found more suitable and sensitive than DAS-ELISA for the detection of BNYVV.

Investigations on pests, diseases and present early warning system of apple orchards in Isparta, Turkey

As a result of three year surveys performed in the apple orchards in Isparta region, 19 pest species belonging to 4 orders were determined and it was found that the main pest was codling moth. Most of the predators and parasitoids were effective against aphids and they were mostly found in the orchards where selective pesticides were used. Apple scab and powdery mildew were the most common and important diseases. Biology of codling moth and apple scab in the region were investigated. By using the obtained data, proper application times were given and some recommendations regarding integrated pest management program in apple orchards, were summarized.

Isparta ve Burdur İlleri Üretim Alanlarında Yetiştirilen Domateslerde Domates Lekeli Solgunluk Virüsü’nün Tanılanması

Isparta ve Burdur illeri domates uretim alanlarinda 2014 yilinda sorvey calismalari gerceklestirilmistir. Bu surveyler sirasinda domates uretim alanlarindan 78 yaprak ornegi toplanmis ve bu ornekler ticari poliklonal antiserumlar kullanilarak DAS-ELISA yontemi ile Domates Lekeli Solgunluk Virusu ( Tomato Spotted Wilt Virus, TSWV) icin test edilmistir. DAS-ELISA testi sonucunda orneklerin hepsi TSWV bakimindan negatif bulunmustur. Daha sonra bu orneklerden RT-PCR (Test Transkripsiyon polimeraz zincir reaksiyonu) yontemi kullanilarak TSWV’e ait RdRp genleri cogaltilarak 78 ornekten 15 tanesinin enfekteli oldugu tespit edilmistir. Yapilan bu calisma TSWV’nun teshisinde RT-PCR yonteminin DAS-ELISA yonteminden daha hassas oldugunu gostermistir.