S. Barbieri

Results of testing the prototype of the AWIN welfare assessment protocol for dairy goats in 30 intensive farms in Northern Italy

Abstract The AWIN project aimed at developing an on-farm welfare assessment protocol for adult dairy goats. A prototype protocol was tested in 30 intensive dairy goat farms to evaluate its feasibility in farms of different size. Time for applying the prototype was recorded and any other constraint was taken into account. Moreover, data collected during the prototype testing provided information on the prevalence of welfare issues in intensive dairy goat farms in Northern Italy. The prototype included 25 animal-based indicators (14 group- and 11 individual-level indicators). The prototype showed a good on-farm feasibility and it was highly accepted among stakeholders, as its application did not interfere with the daily routine. Approximately 2 h were required for the application of the prototype. When feeding racks were available, using them for locking the animals during the individual assessment resulted advantageous to speed the data collection and to reduce handling stress to the goats and disturbance to the farmers. Farm size and different management systems influenced the prevalence of some indicators, with small farms in general better welfare conditions compared to larger farms. The results of the present study represent an important starting point to set up an epidemiological database that may lead to improve the welfare status of goats.

Welfare assessment of horses: the AWIN approach

The EU-funded Animal Welfare Indicators (AWIN) research project (2011–2015) aimed to improve animal welfare through the development of practical on-farm animal welfare assessment protocols. The present study describes the application of the AWIN approach to the development of a welfare assessment protocol for horses (Equus caballus). Its development required the following steps: (i) selection of potential welfare indicators; (ii) bridging gaps in knowledge; (iii) consulting stakeholders; and (iv) testing a prototype protocol on-farm. Compared to existing welfare assessment protocols for other species, the AWIN welfare assessment protocol for horses introduces a number of innovative aspects, such as implementation of a two-level strategy focused on improving on-farm feasibility and the use of electronic tools to achieve standardised data collection and so promote rapid outcomes. Further refinement to the AWIN welfare assessment protocol for horses is needed in order to firstly gather data from a larger reference population and, secondly, enhance the welfare assessment protocol with reference to different horse housing and husbandry conditions.

An innovative tool for on-farm data collection and information sharing

The CoreOrganic2 research project ProPIG is carried out in 75 organic pig farms in 8 European countries (AT; CH; CZ; DE; DK; FR; IT; UK) to improve animal health, welfare and nutrition using farm customised strategies. For future on-farm application (e.g. advisory/certification activities, which are mostly carried out during one day visits), a practical, automatic recording and feedback tool was developed. This should allow on-farm data collection, import of data into a database and the possibility for benchmarking, including a printed output for the farmer to facilitate immediate discussion of results and improvement strategies. To document not only animal health and welfare, but also integrate diet composition and productivity data, it was important to choose key indicators from all areas, which would be available across all eight countries. Based on existing on-farm welfare assessment protocols (e.g. WelfareQuality®, CorePIG) indicators were selected by the consortium, which were then transferred into a Software programme (“PigSurfer”). This Software is available as Desktop- or Android version to be used on Tablet PCs, so that on-farm data (interview with farmer; direct observations on weaners, finishers, sows; productivity and treatment records; feed) can be entered directly. During two visits a database was built and a “Farm report” was printed for each farm, with benchmarking of results, feedback and discussion with the farmer. After a year, “PigSurfer” was used to carry out the following complete process during one day visits across Europe: Surveillance of health and welfare, feedback of data in comparison with results from the previous year as well as benchmarking with 75 other pig farms and printing a report. “PigSurfer” is a promising tool for communicating health and welfare, as it provides not only a database, which can be continuously extended, but is an important step to move from research to on-farm application across Europe.

Recognised-by-law versus other identification systems in pigs: piglets discomfort evaluation and performance testing

The aim of the study was to evaluate the performance of recognised by Italian law (tattoos) and other (ear tags and injectable transponders) identification systems and to investigate if they caused different levels of short-term discomfort in piglets. Ninety-six ten-day-old piglets — equally assigned to four experimental groups — were identified with electronic ear tags (EET), transponders injected in the auricle base (TAB), in intraperitoneal position (TIP), or ear tattoo (T). Losses, breakages and readability of the identification devices were recorded to evaluate their efficiency. Fortyeight piglets were observed continuously for 5 min after identification. Behavioural indicators of pain, discomfort or ease were recorded in terms of duration of occurrence or frequency. Non-parametric analyses of variance were used to compare time required for the application of the devices and behavioural reactions of piglets. No healing problems or breakages of the electronic devices were observed during the trial. The readability after nine months was 93.8% for EET and TIP, 86.7% for TAB and 0% for T. The time required for the application differed significantly (P<0.001) among the four identification devices. EET and TIP showed better results than T considering losses, breakages, readability and time required for application. Behaviours most frequently recorded were head shaking and ear scratching, observed more frequently in piglets after the application of EET (P<0.001). The application of these identification devices caused a mild discomfort in piglets; further studies should investigate in detail the intensity and duration of reactions to the identification procedure.

Deep intrauterine insemination in sow: results of a field trial

Riassunto Traditional insemination techniques in pigs depose a high number of spermatozoa (2 to 3x109 spermatozoa) in a large volume of liquid (80-100 ml) into the cervix channel. The dose can be reduced markedly deposing it directly into the uterine horn. Previous studies showed that fertility rate and litter size were not significantly different with 5 or 15x107 spermatozoa in 10 ml into the uterus. The goal of this study was to determine the on-farm application and the reproductive performance of the deep intrauterine insemination (Firflex® probe, MAGAPOR, Spain) in sows. Experiments were conducted under field conditions in 4 commercial pig farms in the North of Italy (September 2003 and March 2004). A total of 166 crossbred multiparous sows were randomly selected after weaning and assigned to one of the following groups: Group 1 – traditional insemination with 3x109 sperm./dose, two insemination per oestrus (n=94) and Group 2 – deep intrauterine insemination with 15x107 sperm./dose, one insemination per oestrus (n=72). Different technicians in each farm performed the inseminations. Fertility rate was verified by transcutaneous ultrasonography; the total number of born and the number of alive born were counted at farrowing. Analysis of variance using the GLM procedure of SAS (SAS/STAT, 2000) was used to determine the effect of insemination technique, parity, period, farm and their interaction on the main effects of fertility rate and litters size. Fertility rate of Group 2 (50.5%) was significantly lower (P≤0.01) than those achieved after a traditional insemination (77.8%). The interaction between the insemination group and the period of trial was significant (P≤0.01), pointing out increasing results during the second period for deep intrauterine insemination. Prolificacy was significant lower (total born, P≤0.05, and born alive, P≤0.01) for Group 2. In conclusion, the results indicate that deep intrauterine insemination might realize suitable results with respect of fertility, even if the adverse effects on prolificacy will need to be further tested. The potential advantage for the pig industry occurs if technicians acquire the specific practice.

Electronic identification in rabbits: two body sites of injectable transponder

Abstract Identification and monitoring of farm animals are effective tools for traceability. Actual livestock identification systems (ear-tags, tattoos, etc.) are not completely efficient and offer some inconveniences. The use of injectable transponders might be a reliable method for individual identification, allowing the improvement of the data recording and the farm management. The aim of this experiment was to evaluate the applicability of injectable transponder in live rabbits. A total of 40 fattening rabbits of 55 days old were injected with 23 mm passive FDX-B transponders (Datamars, Switzerland), preloaded into a needle in a sterilised packaged and applied by using a transponder injector (Planet ID, Germany). Two body locations for transponder’s injection were studied: laterally to the neck (n=20) and armpit (n=20). Readings of the transponders were performed using portable ISO transceiver, before injection, after injection, the day after, after one week, after one month, before the slaughtering process. Rabbits were weighted at the injection, one week and one month after injection, and at slaughtering; a control group of not treated animals were weight to compare the effect of manipulation. Growth performance were studied by analysis of variance, using of GLM procedure. A χ2 test was used to analyse difference in migration rate. Readability of transponders in the fatteners until slaughterhouse was 100%. Recovery of transponder during the slaughtering process was easy with 100% of transponders recovered. Body site of injection didn’t affect the migration rate, nevertheless it was low. In one animal, the transponder was recovered into the scapular muscle. No harmful effects of the transponder injection on the animals were observed. Weight difference of animals was not significant for the two body site of injection, compare to the control group. Preliminary results showed that transponders can be used as a method for traceability and monitoring of live rabbits, allowing the improvement of the management of a farm.

Enrichment devices for undocked heavy pigs: effects on animal welfare, blood parameters and production traits

Abstract Two independent trials were carried out to test the effectiveness of different environmental enrichments in improving Italian heavy pigs’ welfare. Eighty undocked Landrace × Large White barrows (body weight range: 27.1–158.4 kg) were used. In Trial 1, group C1 received a metal chain and group WL wooden logs (placed inside a specifically designed metal rack). In trial two, the enrichments proposed were a hanging chain (C2), and a vegetal edible block (EB) inside the metal rack. General health, animal behaviour, hair cortisol, blood parameters and growth performance were recorded. In Trial 1, WL pigs interacted with the enrichment at a lower extent than C1 (p < .01) and walked less (p < .05). In Trial 2, EB pigs spent less time in lateral recumbency and rooting/exploring the pen floor (p < .05 and p < .01, respectively), and interacted more with the enrichment (p < .001) than C2. Motivation to explore both WL and EB was maintained over time, conversely to what happened to C1 and C2. No differences were observed in hair cortisol concentration, neutrophil/lymphocyte ratio, skin lesions and growth parameters. Tail lesions were higher in WL than in C1 (p < .05) Minor, transient differences were found in plasma biomarkers. Overall, WL had limited effect on behaviour, whereas EB might have reduced floor over-exploration when compared to the chains. It is concluded that, although from an animal behaviour standpoint EB might be promising, regardless of the device used (C, WL or EB), all experimental groups showed similar welfare parameters levels, stimulating the necessity for wider research on enrichment design and function.

Feeding ecology of alpine chamois living in sympatry with other ruminant species

The populations of Alpine chamois Rupicapra rupicapra rupicapra are currently not at risk of extinction, but local population declines have been observed in some areas. Competition with other herbivore species may be one of the causes for this decline. The present research aims at describing the autumnal diet and preferences of Alpine chamois living in sympatry with red deer Cervus elaphus and roe deer Capreolus capreolus in central Italian Alps and to verify the presence of spatial and diet overlap among these herbivore species. We analyzed the rumen content of 35 samples collected during the hunting season from animals culled in Val Fontana. The results were integrated by data previously collected on rumen content of red deer and roe deer and on space use of the three herbivore species in the same study area. We identified 26 species and 15 genera belonging to 21 plant families of the 51 available in the study area. Monocotyledon families were the most frequently represented in chamois diet composition (78.11%), followed by Ericaceae (5.30%), Betulaceae (4.32%) and Cupressaceae (3.37%). All results showed similar diet between genders. Chamois diet was more similar to red deer than to roe deer diet. The diet selection index (W) showed that on the east-facing side of the valley some species (Calluna vulgaris: Wm = 5.27; Juniperus communis: Wm = 4.24; Loiseleuria procumbens: Wm = 4.82) were selected above their availability, perhaps as a consequence of the higher spatial overlap on this side between chamois and red deer, which make similar use of vegetal resources. Although competition among species is unlikely, due to the low population densities, over-exploitation of food resources and food niche overlap may be future limiting factors for local chamois population, and they should be taken into account for the proper management and control of wild and domestic ruminants.

Welfare assessment on farm: what’s new?

1 Animal welfare, Applied ethology and Sustainable production Lab, Department of Veterinary Medicine, University of Milan, Milan, Italy E-mail: monica.battini@unimi.it; elisabetta.canali@unimi.it; michela.minero@unimi.it; silvana.mattiello@unimi.it; sara.barbieri@unimi.it; valentina.ferrante@unimi.it; emanuela.dallacosta@unimi.it. francesca.dai@unimi.it 2 Neiker-Tecnalia, Arkaute Agrifood Campus, Animal Production, Vitoria-Gasteiz, Spain E-mail: iestevez@neiker.eus, rruiz@neiker.eus, ibeltran@neiker.eus 3 SRUC, Scotland’s Rural College, Edinburgh, United Kingdom E-mail: cathy.dwyer@sruc.ac.uk 4 Department of Preventive Veterinary Medicine and Animal Health School of Veterinary Medicine and Animal Science, University of São Paulo E-mail: Pirassununga, Brasil.adroaldo.zanella@usp.br

Qualitative behavioural assessment of intensive and extensive goat farms

Chronic stress is a long-lasting negative emotional state which induces negative consequences on animals’ behavior. This study aimed at assessing whether unpredictable and repeated negative stimuli (URNS) influence feeding behavior in quail, and whether this can be modulated by their emotionality. Two lines of quail divergently selected on their inherent emotionality (low emotionality, STI; high emotionality, LTI) were either daily exposed to URNS or undisturbed from 17 to 40 days of age (n=32 for each line, in each group). During this time, quail were submitted twice to a sequential feeding procedure: they were offered a hypocaloric diet (7% less caloric than the normocaloric diet) on odd days and a hypercaloric diet (7% more caloric) on even days, for 8 days; then, they received a normocaloric diet (metabolizable energy=12.56 MJ) for 3 days. This sequential feeding procedure was used to assess anhedonia and diet preferences thanks to choice tests (hypo vs. hypercaloric diets) performed at the end of each period. Short-term (30 min) and daily intake were also measured each day. Behavioral tests were performed to assess quail’s emotional reactivity. Results showed that URNS enhanced quails’ emotional reactivity, e.g. in the reactivity to human test, disturbed quail came later (P=0.011) and spent less time (P 0.1), but URNS reduced their daily intake during the 2nd period (P<0.05). Motivation for each diet (assessed by their short-term intake) was differently affected by URNS during the 2nd period: STI quail decreased their motivation to eat the hypercaloric diet (P<0.01) whereas LTI increased their motivation to eat the hypocaloric diet (P<0.01). In conclusion, both lines of quail experienced a chronic stress as URNS induced an increase of their emotional reactivity. Interestingly, URNS induced opposite changes in quail’s feeding behavior: LTI disturbed quail seemed to express a short-term compensatory behavior because of their high motivation to eat, whereas STI disturbed quail seemed to be in a devaluation process as shown by their anhedonia and their decrease of daily intake.Farm animals have to adapt to human presence from birth and being handled may lead to fear and stress reactions. It is known that the mother can be used as a postnatal model in the development of young-human relationship. Through her, some information like auditory ones may even be learnt prenatally. We tested this idea in pigs because they communicate a lot by acoustic signals. The hypotheses were that prenatal experience with human voice could modify behavioural reactions to the experienced voice and to an unfamiliar voice expressing different emotions. We worked with 30 pregnant sows from the last month of gestation. Ten sows (treatment A) were submitted to recordings of human voices during handling: vA during positive interactions and vB during negative interactions, twice a day, 5 days a week, for 10 minutes. Ten other sows (treatment B) received the contrary, i.e. vB during positive interactions and vA during negative interactions. Ten last sows (treatment C) received no vocal stimulations during handling sessions. Two days old piglets (36 A, 39 B, 35 C) were submitted to a 5 min choice test between voices vA and vB in a testing pen (2×1 m). Each voice was played back through loudspeakers positioned at each end of the pen. At 15-18 days of age, 20 other piglets from each treatment were tested in the same conditions except that we played back the voice of an unknown person, reading the same text with a joyful or angry intention. In both tests we recorded vocalisations and locomotion. Data were analysed using non parametric statistics (Statview). In both tests, A and B piglets started to move sooner (P 0.05) to be and the time spent (178 s (46s)) close to the loudspeakers did not depend on the treatment (P>0.05). We also found no difference between the time spent close to one loudspeaker or the other, neither for vA versus vB, nor for joyful versus angry intention (P>0.05). The results show that the prenatal experience of human voice reduces postnatal behavioural reactions of stress (vocalisations, latency to move) during the playback human voices. However, it does not seem to induce specific attraction toward human voice, or human emotional intention. Therefore prenatal experience with human voice may be a good way of reducing fear reactions to human voice after birth.