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Featured researches published by Shao Zy.


International Journal of Nanomedicine | 2012

Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells

Lei Wang; Haijun Zhang; Baoan Chen; Guohua Xia; Shuai Wang; Jian Cheng; Shao Zy; Chong Gao; Wen Bao; Liang Tian; Yanyan Ren; Peipei Xu; Xiaohui Cai; Ran Liu; Xuemei Wang

Traditional Chinese medicine is gradually becoming a new source of anticancer drugs. One such example is wogonin, which is cytotoxic to various cancer cell lines in vitro. However, due to its low water solubility, wogonin is restricted to clinical administration. Recently, the application of drug-coated magnetic nanoparticles (MNPs) to increase water solubility of the drug and to enhance its chemotherapeutic efficiency has attracted much attention. In this study, wogonin was conjugated with the drug delivery system of MNPs by mechanical absorption polymerization to fabricate wogonin-loaded MNPs. It was demonstrated that MNPs could strengthen wogonin-induced cell inhibition, apoptosis, and cell cycle arrest in Raji cells by methylthiazol tetrazolium assay, flow cytometer assay, and nuclear 4′,6-diamidino-2-phenylindole staining. Furthermore, the molecular mechanisms of these phenomena were explored by western blot, in which the protein levels of caspase 8 and caspase 3 were increased significantly while those of survivin and cyclin E were decreased significantly in wogonin-MNPs group. These findings suggest that the combination of wogonin and MNPs provides a promising strategy for lymphoma therapy.


International Journal of Nanomedicine | 2009

Synergistic effect of magnetic nanoparticles of Fe 3 O 4 with gambogic acid on apoptosis of K562 leukemia cells

Baoan Chen; Yiqiong Liang; Weiwei Wu; Jian Cheng; Guohua Xia; Feng Gao; Ding Jh; Chong Gao; Shao Zy; Guo-hong Li; Wen-ji Chen; Xu Wl; Xin-chen Sun; Li-jie Liu; Xiaomao Li; Xuemei Wang

Gambogic acid (GA) has a significant anticancer effect on a wide variety of solid tumors. Recently, many nanoparticles have been introduced as drug-delivery systems to enhance the efficiency of anticancer drug delivery. The aim of this study was to investigate the potential benefit of combination therapy with GA and magnetic nanoparticles of Fe3O4 (MNPs-Fe3O4). The proliferation of K562 cells and their cytotoxicity were evaluated by MTT assay. Cell apoptosis was observed and analyzed by microscope and flow cytometry, respectively. Furthermore, real-time polymerase chain reaction and Western blotting analyses were performed to examine gene transcription and protein expression, respectively. The results showed that MNPs-Fe3O4 dramatically enhanced GA-induced cytotoxicity and apoptosis in K562 cells. The typical morphological features of apoptosis treated with GA and MNPs-Fe3O4 were observed under an optical microscope and a fluorescence microscope, respectively. The transcription of caspase-3 and bax gene in the group treated with GA and MNPs-Fe3O4 was higher than that in the GA-alone group or MNPs-Fe3O4-alone group, but the transcription of bcl-2, nuclear factor-κB, and survivin degraded as did the expression of corresponding proteins in K562 cells. Our data suggests a potential clinical application of a combination of GA and MNPs-Fe3O4 in leukemia therapy.


International Journal of Nanomedicine | 2011

Effect of magnetic Fe3O4 nanoparticles with 2-methoxyestradiol on the cell-cycle progression and apoptosis of myelodysplastic syndrome cells

Guohua Xia; Baoan Chen; Ding Jh; Chong Gao; Huixia Lu; Shao Zy; Feng Gao; Xuemei Wang

This study aims to evaluate the potential benefit of combination therapy of 2-methoxyestradiol (2ME) and magnetic nanoparticles of Fe3O4 (MNPs-Fe3O4) on myelodysplastic syndrome (MDS) SKM-1 cells and its underlying mechanisms. The effect of the unique properties of tetraheptylammonium-capped MNPs-Fe3O4 with 2ME on cytotoxicity was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell-cycle distribution and apoptosis were assessed by flow cytometry. The expression of cell-cycle marker protein was measured by Western blotting. Growth inhibition rate of SKM-1 cells treated with the 2ME-loaded MNPs-Fe3O4 was enhanced when compared with 2ME alone. 2ME led to an increase of caspase-3 expression, followed by apoptosis, which was significantly increased when combined with an MNPs-Fe3O4 carrier. Moreover, the copolymer of 2ME with MNPs- Fe3O4 blocked a nearly two-fold increase in SKM-1 cells located in G2/M phase than in 2ME alone, which may be associated with an accompanying increase of p21 as well as a decrease in cyclin B1 and cdc2 expression, but there was no obvious difference between the MNPs-Fe3O4 and control group. These findings suggest that the unique properties of MNPs-Fe3O4 as a carrier for 2ME, a new anticancer agent currently in clinical trials, may be a logical strategy to enhance the therapeutic activity of MDS.


Molecular Medicine Reports | 2015

Wogonin inhibits the proliferation of myelodysplastic syndrome cells through the induction of cell cycle arrest and apoptosis

Huixia Lu; Feng Gao; Guofang Shu; Guohua Xia; Shao Zy; Hangqin Lu; Keping Cheng

The present study aimed to assess the effects of the flavonoid, wogonin, and its underlying mechanism on myelodysplastic syndrome (MDS) in SKM-1 cells. In the present study, wogonin inhibited the cell proliferation of SKM-1 cells in a dose- and time-dependent manner, with the concentration required to yield a half maximal inhibitory concentration (IC50) of 212.1 µmol/l at 24 h, and 43.4 µmol/l at 72 h. Furthermore, wogonin induced cell cycle arrest at the G0/G1 phase and induced the apoptosis of the SKM-1 cells, which possibly accounted for the antiproliferative effects of wogonin. Notably, the data in the present study revealed that wogonin upregulated the expression of p21Cip1 and p27Kip1, and downregulated the expression of cyclin D1 and cyclin-dependent kinase 4, causing a G0/G1 phase arrest, halting cell cycle progression, and inducing apoptosis in the MDS cells, which was mediated by the mitochondrial pathway through a modulation of the ratio of Bcl-2 to Bax. Therefore, the present study suggests that wogonin may be a logical therapeutic target in the treatment of MDS.


Journal of Experimental Hematology | 2007

Mechanism of 2-methoxyestradiol-induced apoptosis in myelodysplastic syndrome MUTZ-1 cell line.

Xia Gh; Chen Ba; Shao Zy; Lu Hx; Konstanze D; Hartmut D


Journal of Experimental Hematology | 2010

Effects of magnetic nanoparticle of Fe3O4 on apoptosis induced by Gambogic acid in U937 leukemia cells.

Liang Yq; Chen Ba; Wu Ww; Feng Gao; Guohua Xia; Shao Zy; Jian Cheng; Ding Jh; Gao C; Li Gh; Wen-ji Chen; Chen Nn; Xu Wl; Sun Xc; Liu Lj; Li Xm; Wang Xm


Journal of Experimental Hematology | 2012

Reversal Effect of Gambogic Acid on Multidrug Resistance of K562/A02 Cell Line

Tian L; Jun Liu; Chen Ba; Jian Cheng; Ding Jh; Wang S; Guohua Xia; Gao F; Shao Zy; Zhang Hj; Guo Ql; Zhang Hw; Wang L; Ren Yy; Cai Xh; Liu R


Journal of Experimental Hematology | 2008

Sodium valproate synergizes adriamycin to inhibit proliferation and induce apoptosis in myelodysplastic syndrome cell line

Yu C; Chen Ba; Gao C; Ding Jh; Guohua Xia; Shao Zy; Feng Gao; Sun Yy; Jian Cheng; Zhao G; Jun Wang; Song Hh; Ma Y; Bao W


Journal of Experimental Hematology | 2001

[Effect of Autologous or Allogeneic Anti-CD3 Monoclonal Antibody Activated Killer Cells on Normal Hematopoietic Cells]

Chen Ba; Chen Sn; Li Cp; Xia S; Jin Bc; Jun Wang; Shao Zy; Gao C; Ding Jh; Sun Yy


Journal of Experimental Hematology | 2010

[Effect of sodium valproate on human myelodysplastic syndrome cell line SKM-1 and its mechanism].

Chen Ba; Zhang B; Li Cr; Zhou Jf; Wu Xq; Gao C; Feng Gao; Guohua Xia; Shao Zy; Ding Jh; Zhao G; Chen J; Jun Wang; Song Hh; Bao W

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Ding Jh

Southeast University

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Feng Gao

Southeast University

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Chen Ba

Southeast University

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Song Hh

Southeast University

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Jun Wang

Chinese Academy of Sciences

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Jun Wang

Chinese Academy of Sciences

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