Søren E. Larsen

Climate change effects on nitrogen loading from cultivated catchments in Europe: implications for nitrogen retention, ecological state of lakes and adaptation

Climate change might have profound effects on the nitrogen (N) dynamics in the cultivated landscape as well as on N transport in streams and the eutrophication of lakes. N loading from land to streams is expected to increase in North European temperate lakes due to higher winter rainfall and changes in cropping patterns. Scenario (IPCC, A2) analyses using a number of models of various complexity for Danish streams and lakes suggest an increase in runoff and N transport on an annual basis (higher during winter and typically lower during summer) in streams, a slight increase in N concentrations in streams despite higher losses in riparian wetlands, higher absolute retention of N in lakes (but not as percentage of loading), but only minor changes in lake water concentrations. However, when taking into account also a predicted higher temperature there is a risk of higher frequency and abundance of potentially toxic cyanobacteria in lakes and they may stay longer during the season. Somewhat higher risk of loss of submerged macrophytes at increased N and phosphorus (P) loading and a shift to dominance of small-sized fish preying upon the key grazers on phytoplankton may also enhance the risk of lake shifts from clear to turbid in a warmer North European temperate climate. However, it must be emphasised that the prediction of N transport and thus effects is uncertain as the prediction of regional precipitation and changes in land-use is uncertain. By contrast, N loading is expected to decline in warm temperate and arid climates. However, in warm arid lakes much higher N concentrations are currently observed despite reduced external loading. This is due to increased evapotranspiration leading to higher nutrient concentrations in the remaining water, but may also reflect a low-oxygen induced reduction of nitrification. Therefore, the critical N as well as P loading for good ecological state in lakes likely has to be lower in a future warmer climate in both north temperate and Mediterranean lakes. To obtain this objective, adaptation measures are required. In both climate zones the obvious methods are to change agricultural practices for reducing the loss of nutrients to surface waters, to improve sewage treatment and to reduce the storm-water nutrient runoff. In north temperate zones adaptations may also include re-establishment of artificial and natural wetlands, introduction of riparian buffer zones and re-meandering of channelised streams, which may all have a large impact on, not least, the N loading of lakes. In the arid zone, also restrictions on human use of water are urgently needed, not least on the quantity of water used for irrigation purposes.

Biomanipulation as a Restoration Tool to Combat Eutrophication: Recent Advances and Future Challenges

Eutrophication resulting from high nutrient loading has been the paramount environmental problem for lakes world-wide for the past four decades. Efforts are being made in many parts of the world to reduce external nutrient loading via improved wastewater treatment or diversion of nutrient-rich inflows. However, even after a reduction of the external phosphorus loading, the effects obtained may be unsatisfactory. This may reflect an insufficient reduction in the external nutrient loading to effectively limit phytoplankton growth. However, the lack of success may also be due to chemical or biological within-lake inertia preventing or delaying improvements. To overcome the resilience and thereby reinforce recovery, a number of physico-chemical and biological restoration methods have been developed. In this chapter, we describe recent developments of biological restoration methods related to eutrophication, their short-term and long-term effects, and discuss the possibility of using combined physico-chemical and biological methods to improve the long-term stability of restoration and to reduce restoration costs. As comprehensive reviews of the effect of fish manipulation in cold temperate lakes are numerous, for these waterbodies, we highlight recent results, including effects on biodiversity and metabolism, and present new approaches of biomanipulation. Our particular focus is, however, directed at biomanipulation in warm lakes and on combined treatments which are far less well described in the literature.

Ensemble modelling of nutrient loads and nutrient load partitioning in 17 European catchments

An ensemble of nutrient models was applied in 17 European catchments to analyse the variation that appears after simulation of net nutrient loads and partitioning of nutrient loads at catchment scale. Eight models for N and five models for P were applied in three core catchments covering European-wide gradients in climate, topography, soil types and land use (Vansjø-Hobøl (Norway), Ouse (Yorkshire, UK) and Enza (Italy)). Moreover, each of the models was applied in 3-14 other EUROHARP catchments in order to inter-compare the outcome of the nutrient load partitioning at a wider European scale. The results of the nutrient load partitioning show a variation in the computed average annual nitrogen and phosphorus loss from agricultural land within the 17 catchments between 19.1-34.6 kg N ha(-1) and 0.12-1.67 kg P ha(-1). All the applied nutrient models show that the catchment specific variation (range and standard deviation) in the model results is lowest when simulating the net nutrient load and becomes increasingly higher for simulation of the gross nutrient loss from agricultural land and highest for the simulations of the gross nutrient loss from other diffuse sources in the core catchments. The average coefficient of variation for the model simulations of gross P loss from agricultural land is nearly twice as high (67%) as for the model simulations of gross N loss from agricultural land (40%). The variation involved in model simulations of net nutrient load and gross nutrient losses in European catchments was due to regional factors and the presence or absence of large lakes within the catchment.

Subjective reactions to volatile organic compounds as air pollutants

Abstract Human subjective reactions to indoor air pollution in the form of volatile organic compounds in five concentrations ⩽ mg m −3 were examined in a climate chamber under controlled conditions in a balanced experimental design. The reactions of 25 subjects were registered in two questionnaires containing 25 and six questions and on a linear analogue rating scale. Each subject was tested for one day including four runs in each of the five treatments of 50 min duration. Dose effects were found for perceived odour intensity at 3 mgm −3 . Air quality, need for ventilation, irritation of eye and nose showed significant effect at 8 mg m −3 . Significant reduced well being was reported at 25 mgm −3 . The analyses indicated that lower threshold for some of these effects would have been found if more subjects or longer exposure-times had been used. Gender, age, occupational education and smoking habits were co-factors for many of the symptoms reported.

Plant distribution and abundance in relation to physical conditions and location within Danish stream systems

The distribution of obligate submerged plants, amphibious plants and terrestrial plants in streams was examined in relation to water depth, substrate and distance to the banks using univariate and mulitivariate analyses. The analyses were based on recordings in more than 40 000 quadrats (25×25 cm) in 208 unshaded sites in the predominantly small and shallow (<1.6 m) Danish streams. Also, the distribution of plant abundance and richness from the source to the outlet of the stream system was determined.The submerged plants in Danish streams include 87 terrestrial, 22 amphibious and 30 obligate submerged taxa. The distribution of plant types was mainly related to water depth and distance to the bank among the physical conditions, while the type of bottom substrate had no significant influence. Terrestrial plants and amphibious plants (excluding Sparganium emersum) dominated in shallow water near the bank, but declined rapidly with increasing depth and distance to the bank, reflecting the importance of dispersal by ingrowth from populations on the banks to the water among these plants. Accordingly, these two plant groups constituted a higher proportion of total plant abundance in small streams than large streams. S. emersum dominated on great depth and distance to the banks, probably reflecting the lengthwise dispersal of this species from upstream to downstream parts of the stream system, the tolerance of the species to weed cutting and the adaptation to grow at low light intensities. Obligate submerged plants dominated at intermediate depths and at all distances from the bank except from 0 to 50 cm. This distribution reflects the ability of these species to disperse lengthwise in streams and live permanently submerged.The species number of all species and obligate submerged plants was lower in the smallest stream sites compared to larger downstream sites, while there was no difference for terrestrial and amphibious plants. The downstream increase of submerged species can be explained by the increase of habitat area and the dispersal of plants with the current, implying that the species pool accumulates with distance from the source. This result is at variance with a maximum richness of submerged plant species predicted for intermediate-sized streams according to the River Continuum Concept developed for large North American streams having forested shallow upstream sections and unshaded, deep downstream sections both unsuitable to submerged plant growth. The results for Danish streams imply that both the longitudinal connection through the flowing water and the transversal connection to the local species on the banks are important for plant distribution in the streams.

Phosphorus Load to Surface Water from Bank Erosion in a Danish Lowland River Basin

Phosphorus loss from bank erosion was studied in the catchment of River Odense, a lowland Danish river basin, with the aim of testing the hypothesis of whether stream banks act as major diffuse phosphorus (P) sources at catchment scale. Furthermore, the study aimed at analyzing the impact of different factors influencing bank erosion and P loss such as stream order, anthropogenic disturbances, width of uncultivated buffer strips, and the vegetation of buffer strips. A random stratified procedure in geographical information system (GIS) was used to select two replicate stream reaches covering different stream orders, channelized vs. naturally meandering channels, width of uncultivated buffer strips (≤ 2 m and ≥ 10 m), and buffer strips with different vegetation types. Thirty-six 100-m stream reaches with 180 bank plots and a total of 3000 erosion pins were established in autumn 2006, and readings were conducted during a 3-yr period (2006-2009). The results show that neither stream size nor stream disturbance measured as channelization of channel or the width of uncultivated buffer strip had any significant ( < 0.05) influence on bank erosion and P losses during each of the 3 yr studied. In buffer strips with natural trees bank erosion was significantly ( < 0.05) lower than in buffer strips dominated by grass and herbs. Gross and net P input from bank erosion amounted to 13.8 to 16.5 and 2.4 to 6.3 t P, respectively, in the River Odense catchment during the three study years. The net P input from bank erosion equaled 17 to 29% of the annual total P export and 21 to 62% of the annual export of P from diffuse sources from the River Odense catchment. Most of the exported total P was found to be bioavailable (71.7%) based on a P speciation of monthly suspended sediment samples collected at the outlet of the river basin. The results found in this study have a great importance for managers working with P mitigation and modeling at catchment scale.

Macroinvertebrate/sediment relationships along a pesticide gradient in Danish streams

Twenty-nine Danish streams were investigated in September 1998. The streams drained catchments of varying hydrology, topography, soil type and land use. In each stream, the newly accumulated streambed sediment was sampled and subsequently analysed for pesticides. In each stream, five replicate macroinvertebrate samples were taken in the same sediments as the pesticide samples. In addition, five samples were taken in riffles to provide a complete picture of macroinvertebrate community composition. The number of detected pesticides reflected soil type and land use: in agricultural catchments on clay soils the average number of pesticides were 4.3±2.2; on sandy soils 2.6±1.0, while only 1.5±0.6 pesticides were found in streams without agricultural activities. The macroinvertebrate composition showed clear changes along this gradient of sediment pesticide concentrations. The number of the amphipod Gammarus pulex was negatively correlated with sediment pesticide concentration, while the number of Oligochaeta and Hirudinae was positively correlated. The findings indicate that pesticides have a potential to structure macroinvertebrate communities in Danish streams. However, agricultural impact is more than pesticides, and several other factors, such as channelisation, affect the macroinvertebrate community and these are not easily separated.

The effect of brown trout (Salmo Trutta L.) on stream invertebrate drift, with special reference to Gammarus pulex L.

The effect of brown trout (Salmo trutta L.) on invertebrate drift density was examined in three previously fish-free springbrooks. Drift density was studied during the day following the sequential introduction of: 1) empty cages, 2) cages containing trout, and 3) empty cages. Each period lasted three whole days, and drift density was measured at daytime and night-time every day. Control drift density was determined using empty cages in two similar springbrooks.Night-time drift density of large Gammarus pulex L. (>3.9 mg DW ind−1) decreased compared to that of smaller size-groups following trout introduction, and was significantly lower (p<0.05) than the pre-introductory level after the trout had been removed again.The daytime drift density of large G. pulex remained unchanged, as did both daytime and night-time drift density of smaller size-groups of G. pulex, and the other taxa investigated (Leuctra hippopus (Kempny), Leuctra nigra (Olivier), Amphimura sp., Nemoura flexuosa Aubert, and Baetis rhodani (Pict.)). No significant changes in drift density were observed in two control springbrooks.

The search for reference conditions for stream vegetation in northern Europe

Summary 1. The European Water Framework Directive provides a framework for improving the ecological quality of stream ecosystems, with deviation from reference used as a measure of ecological status. 2. Here we examine the possibility of using less impacted stream sites from Latvia, Lithuania and Poland to establish a Danish reference network for macrophyte assemblages, and as a guiding image for identification of possible references sites within Denmark. Both approaches were evaluated using historical Danish records. 3. Four different macrophyte assemblages were identified for mid‐sized streams in the Central and Eastern Lowland ecoregions. Macrophyte assemblages could not be delineated using physical stream site characteristics; however a gradual change in assemblage composition was attributed to differences in alkalinity and human impact. 4. Assemblages of contemporary vegetation in Denmark were quite similar to those found in Polish, Latvian and Lithuanian streams (26–35%). However, more importantly, from species‐based predictions we noted higher similarity, particularly with Latvian and Lithuanian streams, before intensive land use commenced in Denmark (c. 1900). These results show that stream sites from these three countries can be considered in a Danish reference network. 5. Two of the four macrophyte assemblages comprised species such as Fontinalis antipyretica, Myriophyllum spicatum, Nuphar lutea, Potamogeton alpinus and P. perfoliatus that have a very scattered occurrence in the contemporary vegetation in Denmark. These groups were closely associated with the predictions from historic records, thereby lending support the conjecture that these assemblages could be part of the guiding image for the identification of potential reference sites within Denmark.

Long-term effects of stream management on plant communities in two Danish lowland streams

Submerged macrophytes grow abundantly in most shallow streams common in the cultivated lowlands of northwestern Europe. Weed-cutting has been practised for years in many of these streams to reduce the risk of flooding of adjacent land. Our objective was to quantify long-term impacts of weed-cutting on macrophyte communities in two Danish rivers. We found that the total macrophyte coverage was similar in the weed-cut and uncut reaches in the two rivers, but species richness, diversity and patch complexity were higher in the uncut reaches. The spatial distribution of macrophytes on the stream bottom was also more heterogeneous in the uncut stream reaches. We also found evidence of a strong effect of weed-cutting on macrophyte species composition. P. natans was abundant in the uncut reaches in both streams but practically eliminated in the cut reaches, despite the fact that its basic habitat requirements were met. Also, the established phase strategy of the macrophyte community was affected by weed-cutting. Species displaying characteristically ruderal traits were more abundant in the cut reaches and species with competitive abilities were only abundant in the uncut stream reaches. We suggest that important species traits in streams, where the weed is cut regularly, are associated with rapid growth and high dispersal-capacity. Our results indicate that weed-cutting can contribute significantly to a decline in species diversity in streams. To provide optimal conditions for diverse stream macrophyte communities, we therefore suggest that weed-cutting should be minimised.