Takemitsu Matsuo

Identification of a Novel DNA-Binding Protein from Mycobacterium bovis Bacillus Calmette-Guérin

A novel DNA‐binding protein expressed (8–10% in total protein) in Mycobacterium bovis bacillus Calmette‐Guérin was observed. This protein was designated mycobacterial DNA‐binding protein 1 (MDP1). MDP1 recognized bases, sugar moieties, phosphate‐backbone on DNA and preferentially bound to DNA guanine and cytosine. In the gel retardation assay, MDP1 preferentially bound to closed circular plasmid DNA than open circular and linear form plasmid DNA and also bound to RNA. MDP1 formed a highly polymerized structure and localized not only in the nucleoid but also at the 50S ribosomal subunits and cell surface. MDP1 was conserved in Mycobacterium thus far examined and the expression was enhanced in stationary growth phases. These results will provide a reasonable basis for further study of the function of MDP1 in living mycobacteria.

Severe Dental Open Bite Malocclusion With Tongue Reduction After Orthodontic Treatment

We treated a 21-year-old woman with a severe open bite and macroglossia with a standard edgewise appliance and without partial glossectomy. This was followed by retention using a Begg-type plate retainer for the upper dental arch and a fixed canine-to-canine for the lower arch. A crib was added to the upper plate retainer for suppression of a tongue thrust. The lower arch relapsed during the retention period, with a widening of the intermolar distance, flaring of the anterior teeth, and increased mobility of the teeth. We chose tongue reduction to resolve these problems and one-third of the middle dorsal part of the tongue was excised. After the tongue reduction, the patient experienced no functional problem in mastication, swallowing, and gustation, but she complained of mild speech difficulty and slight pain on the dorsal portion of her tongue. These symptoms disappeared 6 months after surgery. At this time, the mandibular dental arch was markedly improved. The flared lower dental arch had returned to an upright position and the tooth mobility reduced to normal. No appliance was used after surgery. Most of the recovery changes occurred within 4 months. This case highlights the importance of the teeth tending to move toward a balance between the tongue pressure from the inside and labio-buccal pressure from the outside.

Mucoepidermoid carcinoma arising in Warthin's tumor of the parotid gland

Malignant transformation of Warthins tumor is extremely rare, although it is the second most common benign tumor of the parotid gland. We describe our experience of mucoepidermoid carcinoma arising in Warthins tumor of the parotid gland in a 64‐year‐old man. He had a swelling in the left parotid‐masseteric region. The removed tumor was well encapsulated and histopathologically comprised Warthins tumor and low‐grade mucoepidermoid carcinoma. The mucoepidermoid carcinoma infiltrated lymphoid stroma of the Warthins tumor, but capsular invasion is not found. Considering the clinical course and physical examination, the lesion was diagnosed as mucoepidermoid carcinoma arising in Warthins tumor because its features filled the criteria of malignant transformation of Warthins tumor. There has been no recurrence or distant metastasis for 19 months. Histological change from Warthins tumor to mucoepidermoid carcinoma may be implicated in squamous or goblet cell metaplasia of epithelial cells. It is considered that the neoplastic cells of Warthins tumor acquire malignant genotypes simultaneously with this dual differentiation.

Differential transcription of the MPB70 genes in two major groups of Mycobacterium bovis BCG substrains

Substrains of Mycobacterium bovis BCG (BCG) have been divided into two major groups, high and low producers, on the basis of the amount of secretion of the MPB70 protein. The antigen is produced in high concentration by BCG Tokyo, Moreau, Russia and Sweden (high-producer substrains), whereas in BCG Pasteur, Copenhagen and Tice (low-producer substrains) it is detected at 1% (w/w) or less of the concentration of BCG Tokyo. To investigate why this protein is secreted differently, the MPB70 genes of BCG Tokyo and Pasteur were cloned, sequenced and compared. The MPB70 genes in two substrains showed exactly the same sequence. Even the upstream and downstream regions of the MPB70 gene were identical. MPB70 gene expression was assessed by means of Northern hybridization analysis and reverse transcriptase polymerase chain reaction. The mRNA was clearly detected in BCG Tokyo, but a very low level in BCG Pasteur. On the basis of these results, the difference in the secretion of the MPB70 protein between BCG Tokyo and Pasteur was attributed to differential transcription efficiencies.

An animal model for subperiosteal tissue expansion

An animal model for subperiosteal tissue expansion is described. Small tissue expanders were placed bilaterally and inflated in the buccal subperiosteal pockets of the mandible. Additionally, subcutaneous expansion was performed in the breast region. Twenty-one dogs were used in three experimental groups to evaluate the optimal size of an expander for this model, the optimal inflation regimen, and the histologic changes following subperiosteal expansion. Hemicylindrical expanders, 20 mm in width, 10 mm in height, and 30 mm in length, inflated with 1 mL saline every four days, were used for this model. It was found that the periosteum was replaced by fibrous connective tissue during expansion. Capsule formation following subperiosteal expansion was much more rapid than that following subcutaneous expansion. A greater increase in vascularity was noted in the subperiosteal expansion group and the subperiosteal control group than in the subcutaneous expansion group. In the subperiosteal expansion group, a thick fibrous capsule, with minimal inflammatory response, was observed 1 week after full inflation. Leaving the fully inflated subperiosteal expanders in place more than 1 month accelerated the resorption of the underlying bone. These results support the previously reported empirical regimens of subperiosteal tissue expansion. Furthermore, the excellence of the subperiosteal expanded bed as a recipient site for onlay grafting was confirmed.

Acute suppurative parotitis caused by a fish bone : a case report

A 57-year-old woman was referred to our clinic because of swelling, redness, and severe pain of the right parotid region. Examination revealed swelling with redness of the papilla of Stensens duct and some pus discharge from it. Intravenous Cefotiam 1 g twice a day for 3 days improved the symptoms. After 3 days, a foreign body which appeared to be a fish bone was visible at the opening of the parotid duct (Fig. 1) and was removed with a forceps. Cefotiam was continued for another 2 days for a total of 5 days. Removal of the fish bone resulted in quick resolution of the patients symptoms. Histologic examination revealed needleshaped calcified material consistent with a fish bone. Attached bacterial colonies and cellular components were found around the calcified tissue.

An extensive case of cherubism: A long term follow-up case

The present paper describes the radiological features, including CT, of a case of cherubism followed for a relatively long period. The case demonstrated the active phase of the lesion, which progressed from grade 1 to grade 3 during a 6 year period. The radiographic signs, such as increased radiolucency, inflammatory signs and root resorption as well as laboratory data seemed to be important to diagnose the stage of cherubism and to predict the clinical course of the disease.

Advanced osteosarcoma of maxilla for which palliative treatment was usefull. Report of a case.

高度に進展した上顎骨肉腫の1例を経験した。患者は32歳女性で, 7年前に左上顎良性骨芽細胞腫の摘出術を受けていた。初診時, 巨大な腫瘤は左顔面全体に及んでいた。生検の結果は骨肉腫であり, MTX大量療法ならびに放射線療法を行ったところ, 腫瘍は縮小し, 姑息療法として減量手術 (腫瘍切除術) を施行した。術後, 腫瘍は静止的であったが, その後右上肺野, 右脳に転移が認められるようになった。右脳転移巣に対して定位的脳放射線療法を行ったが, 呼吸不全により死の転帰をとった。剖検の結果, 右上肺野の他に, 右脳, 心臓, 舌, その他に転移巣が確認された。